Anti cdk2 phospho thr160

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-CDK2 phospho-Thr160 is a laboratory reagent that detects the phosphorylation of threonine 160 on the cyclin-dependent kinase 2 (CDK2) protein. This phosphorylation event is important for the activation of CDK2, which plays a critical role in cell cycle regulation.

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Lab products found in correlation

Anti γ tubulin, by Merck Group (4 mentions) Anti lc3a b, by Cell Signaling Technology (3 mentions) Anti cyclin a, by Merck Group (3 mentions) Anti α tubulin t9026, by Merck Group (3 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (2 mentions) Anti cdk2, by Merck Group (2 mentions) Anti h2ax phospho ser139, by Abcam (2 mentions) Anti cyclin e, by Cell Signaling Technology (2 mentions) Anti phospho erk1 2 on thr202 tyr204, by Cell Signaling Technology (2 mentions) Anti erk1 2, by Cell Signaling Technology (2 mentions) Anti phospho chk2 thr68, by Cell Signaling Technology (2 mentions) Anti gapdh gtx100118, by GeneTex (1 mentions) Anti acetylated α tubulin, by Merck Group (1 mentions) Polyclonal anti flag, by Merck Group (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Monoclonal anti flag m2, by Merck Group (1 mentions) Anti dna pkcs, by Santa Cruz Biotechnology (1 mentions) Anti centrin 20h5, by Merck Group (1 mentions) Anti ku70, by GeneTex (1 mentions) Anti cyclin a2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-CDK2 (52 citations) Anti-phospho-CDK2 (4 citations) Phospho-CDK2 (4 citations) Phospho-CDK2 (Thr160) (3 citations)

5 protocols using anti cdk2 phospho thr160

Antibody Panel for Cell Signaling

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The following antibodies were obtained commercially: anti-γ-tubulin (T6557), anti-cyclin A (C4710), and anti-α-tubulin (T9026) (Sigma, St. Louis, MO), anti-CDK2 (#2546), anti-CDK2 phospho-Thr160 (#2561), anti-Cleaved Caspase-3 (Asp175) (#9661), anti-CYP11A1 (D8F4F) (#14217), anti-Ad4BP/SF-1 (STF-1, D1Z2A) (#12800), and anti-LC3A/B (#12741) (Cell Signaling, Beverly, MA), anti-H2AX phospho-Ser139 (ab2893, Abcam, Cambridge, UK), anti- Beclin 1/ATG6 (NB500-249) (Novus, Littleton, CO), anti-cyclin E1 (HE-12, GTX23927), anti-actin (GTX109639) and anti-GAPDH (GTX100118) (Genetex, Irvine, CA).

Syu J.S., Baba T., Huang J.Y., Ogawa H., Hsieh C.H., Hu J.X., Chen T.Y., Lin T.C., Tsuchiya M., Morohashi K.I., Huang B.M., Lu F.L, & Wang C.Y. (2017). Lysosomal activity maintains glycolysis and cyclin E1 expression by mediating Ad4BP/SF-1 stability for proper steroidogenic cell growth. Scientific Reports, 7, 240.

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Characterization of Cellular Proteins

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The following antibodies were obtained commercially: anti-γ-tubulin, polyclonal anti-FLAG, anti-Cyclin A, monoclonal anti-FLAG M2, anti-α-tubulin and anti-acetylated-α-tubulin (all from Sigma, St. Louis, MO), anti-Cyclin E, anti-CDK2 phospho-Thr160, anti-Akt and anti-Akt phospho-Thr308 (Cell Signaling, Beverly, MA), anti-centrin 20H5 (Millipore, Billerica, MA), polyclonal anti-β-catenin and anti-GSK3β (Abcam, Cambridge, UK), anti-Ku70 (Genetex, Trvine, CA), anti-DNA-PKcs, and anti-DNA-PKcs phospho-Thr 2609 (Santa Cruz Biotech, Santa Cruz, CA). The immune sera against SF-1 have been described previously [19 (link)].

Wang C.Y., Lai P.Y., Chen T.Y, & Chung B.C. (2014). NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and β-catenin accumulation. Cell Communication and Signaling : CCS, 12, 55.

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Antibody Immunodetection in Cell Research

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The following antibodies were obtained commercially: anti-acetylated-tubulin (T7451), anti-γ-tubulin (T5326), and anti-α-tubulin (T9026) (Sigma, St. Louis, MO, USA); anti-LC3A/B (D3U4C) XP (#12741), anti-cyclin A2 (#4656), anti-cyclin E1 (HE12; #4129), anti-CDK2 (#2546), and anti-CDK2 phospho-Thr160 (#2561) were purchased from Cell Signaling (Beverly, MA, USA); anti-fetuin-A (ab137125), and anti-Beclin 1 antibody [EPR19662] (ab207612) were purchased from Abcam (Cambridge, UK); anti-actin (AC-15) (GTX26276) was purchased from Genetex (Irvine, CA, USA); anti-IFT88 (13967-1-AP) was purchased from Proteintech (Chicago, IL, USA); and anti-CEP164 (NBP1-81445) was purchased from Novus (Littleton, CO, USA).

Wang C.Y., Su M.T., Cheng H.L., Kuo P.L, & Tsai P.Y. (2019). Fetuin-A Inhibits Placental Cell Growth and Ciliogenesis in Gestational Diabetes Mellitus. International Journal of Molecular Sciences, 20(20), 5207.

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Immunoblotting for Cell Cycle Markers

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The following antibodies were obtained commercially: anti-γ-tubulin (T6557), anti-Cyclin A (C4710) and anti-α-tubulin (T9026) (Sigma), anti-CDK2 (#2546), anti-Chk2 (#6334), anti-Chk2 phospho-Thr68 (#2661), anti-ERK1/2 (#4695), anti-phospho-ERK1/2 on Thr202/Tyr204 (#4370), anti-Cyclin E (#4132), anti-CDK2 phospho-Thr160 (#2561) and anti-LC3A/B (#12741) (Cell Signaling, Beverly, MA, USA), anti-H2AX phospho-Ser139 (ab2893, Abcam, Cambridge, UK), anti-actin (GTX109639) and anti-GAPDH (GTX100118) (Genetex, Irvine, CA, USA).

Chen T.Y., Syu J.S., Lin T.C., Cheng H.L., Lu F.L, & Wang C.Y. (2015). Chloroquine alleviates etoposide-induced centrosome amplification by inhibiting CDK2 in adrenocortical tumor cells. Oncogenesis, 4(12), e180-.

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Antibody-Based Protein Analysis in Cell Models

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The following antibodies were obtained commercially: anti‐γ‐tubulin (T6557), anti‐cyclin A (C4710), and anti‐α‐tubulin (T9026) (Sigma, St. Louis, MO), anti‐ATR, anti‐ATR phospho‐Ser428, anti‐CDK2 (#2546), anti‐CDK2 phospho‐Thr160 (#2561), anti‐cleaved caspase‐3 (Asp175) (#9661), anti‐Chk2, anti‐Chk2 phospho‐Thr68, anti‐AKT, anti‐phospho‐AKT (Ser473), anti‐ERK1/2 (#4695), anti‐phospho‐ERK1/2 on Thr202/Tyr204 (#4370) and anti‐LC3A/B (#12,741) (Cell Signaling, Beverly, MA), anti‐Chk1 phospho‐Ser345 and anti‐H2AX phospho‐Ser139 (ab2893, Abcam, Cambridge, UK), anti‐Ku70 and anti‐ATM (Genetex, Trvine, CA), anti‐ATM phospho‐Ser1981 (Epitomics, Burlingame, CA), anti‐cyclin E (HE‐12, GTX23927), anti‐actin (GTX109639) (GeneTex, Irvine, CA), anti-FAK (EP695Y, ab40794), anti-E cadherin (HECD-1, ab1416), and anti-cyclin F antibody (ab203117) (Abcam, Cambridge, UK).
For drug treatment, the following chemicals are treated for 24 h before analysis. Ku55933 (ATM inhibitor, SML1109, 10 μM), cytochalasin D (C2618, 5 μg/mL), vanillin (V1104, 1 mM), U0126 (U120, 10 μM), and chloroquine (CQ, 50–63-5, 50 μM) were purchased from Sigma, St. Louis, MO. Bafilomycin-A1 (Baf.A1, BML-CM110, 10 nM) was purchased from Enzo, NY, USA.

Wang C.Y., Tsai S.W., Chien H.H., Chen T.Y., Sheu S.Y., So E.C, & Huang B.M. (2020). Cordycepin Inhibits Human Gestational Choriocarcinoma Cell Growth by Disrupting Centrosome Homeostasis. Drug Design, Development and Therapy, 14, 2987-3000.

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