Vitek 2 gn

Non-duplicate P. aeruginosa isolates were collected from clinics and hospitals from the four district municipalities. Specimens included throat swabs, wound swabs, swabs from abscesses, sputum, urine, blood culture and catheter tips. Demographic characteristics of patients and medical histories were collected from medical records including date of specimen collection, gender and age. All samples were routinely cultured on MacConkey and Blood agar plates. Blood and sputum were also cultured on chocolate agar. Suspected colonies were plated on Cetrimide agar and identified by gram staining, colony characteristics, motility, pyocyanin production and characteristics grape-like odour^{41 (link)}. Strains were identified to the species level with Vitek 2 GN (bioMérieux, Inc. USA) ID cards and confirmed by Microscan NID 2 panels (Beckman Coulter, Inc. USA). Specific primers and probes targeting gyrB were amplified by singleplex rPCR and were also used to confirm identity of the isolates.

Our study employed the VITEK^® 2 (BioMerieux, Marcy L’Etoile, France) microbial identification system to identify paracetamol-degrading bacteria in spinach roots and shoots.
Spinach shoot and root samples were weighed under aseptic conditions under a Class II Biosafety Cabinet, surface sterilized with 70% ethanol for 7 min, and washed three times with sterile distilled water. Then, 2.5% bleach was added to the samples for 10 min and they were washed thrice with sterile distilled water. After sterilization, samples were crushed in 10 mL of sterile distilled water. One milliliter of the extract was taken from each sample and serially diluted to 1 × 10⁻⁵. Then, from each diluted sample extract, 100 µL was spread on Luria Bertani (LB) agar plates, wrapped with parafilm, and incubated at 28 °C, and bacterial growth was observed for 72 h. Gram’s staining was used for preliminary identification of the isolated bacterial strains. Bacterial suspensions were prepared in sterile saline, and the density was adjusted using VITEK 2 DensiCheck (BioMerieux, Marcy L’Etoile, France) to a McFarland standard of 0.5–0.63. Gram-positive and Gram-negative bacteria were identified using G.P. and G.N. cards, respectively, via VITEK 2 G.N. (21341 BioMerieux, Marcy L’Etoile, France) and G.P. (21342 BioMerieux, Marcy L’Etoile, France) Identification Kits.

Expectorate sputum was selected as the sampling technique and routine microbiological investigations were conducted at the medical microbiology laboratory using standard bacteriology. All isolates were first identified using the VITEK^® 2 GN and VITEK^® 2 GP ID cards (BioMérieux, Marcy, l’Etoile, France). Antimicrobial susceptibility tests were performed using the VITEK 2 GN AST-N222 and VITEK 2 AST GP 67 cards (BioMérieux, Marcy, l’Etoile, France).