Fluorescein isothiocyanate fitc conjugated anti mouse immunoglobulin

Manufactured by MP Biomedicals

Sourced in United States

Fluorescein isothiocyanate (FITC)-conjugated anti-mouse immunoglobulin is a laboratory reagent used for the detection and analysis of mouse immunoglobulins in various applications such as flow cytometry, immunohistochemistry, and Western blotting. It contains FITC, a fluorescent dye, conjugated to antibodies that bind specifically to mouse immunoglobulins.

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Lab products found in correlation

Remdesivir gs 5734, by Selleck Chemicals (2 mentions) Mab9013, by Merck Group (2 mentions) Methylcellulose, by Merck Group (2 mentions) Digitoxin, by Merck Group (1 mentions) Digoxin, by Merck Group (1 mentions) Oleandrin, by Merck Group (1 mentions) Digitoxigenin, by Merck Group (1 mentions) Crystal violet, by Merck Group (1 mentions) Gs 441524, by Selleck Chemicals (1 mentions) Ouabain, by Merck Group (1 mentions) Alexa fluor 488 goat anti human igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Dapi d1306, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

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FITC (10 citations)

2 protocols using fluorescein isothiocyanate fitc conjugated anti mouse immunoglobulin

Antiviral Compound Characterization Protocol

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DMSO (≧99.5%), digoxin (D6003, ≧95%, HPLC), digitoxin (D5878, ≧92%, HPLC), digitoxigenin (D9404, 99%, TLC), ouabain (O3125, ≧95%, HPLC), oleandrin (O9640, ≧98%, HPLC), crystal violet (C0775, dye content ≥90%), and methylcellulose (#M0387) were purchased from Sigma-Aldrich (St. Louis, MO, United States); bufalin (15725, ≧ 98%, HPLC) from Cayman Chemical (Ann Arbor, MI, United States); digoxin-BSA (80-ID10, HPLC) from Fitzgerald Industries (Acton, MA, United States); rostafuroxin (T2621, ≧99%, HPLC) from Target Molecule Corp. (Boston, MA, United States); istaroxime hydrochloride (HY-15718A, ≧99%, HPLC) from MedChem Express (Monmouth Junction, NJ, United States); and Remdesivir (GS-5734) (S8932, 99.3%, HPLC) and GS-441524 (S6814, 99.3%, HPLC) were from Selleckchem (Houston, TX, United States). The antibody against nucleocapsid proteins of HCoV-OC43 (Mab9013) was purchased from Merck Millipore (Burlington, MA, United States), and fluorescein isothiocyanate (FITC)-conjugated anti-mouse immunoglobulin (#55499) from MP Biomedicals (Irvine, CA, United States). Anti-SARS-CoV-2 N protein antibodies were provided by Dr. An-Suei Yang of the Genomics Research Center, Academia Sinica. Goat anti-human IgG-Alexa Fluor 488 (A11013) and DAPI (D1306) were purchased from Invitrogen. 10% formaldehyde solution was purchased from Marcon™ Chemicals (#H121-08).

Yang C.W., Lee Y.Z., Hsu H.Y., Jan J.T., Lin Y.L., Chang S.Y., Peng T.T., Yang R.B., Liang J.J., Liao C.C., Chao T.L., Pang Y.H., Kao H.C., Huang W.Z., Lin J.H., Chang C.P., Niu G.H., Wu S.H., Sytwu H.K., Chen C.T, & Lee S.J. (2020). Inhibition of SARS-CoV-2 by Highly Potent Broad-Spectrum Anti-Coronaviral Tylophorine-Based Derivatives. Frontiers in Pharmacology, 11, 606097.

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Antibody Staining and Microscopy Protocol

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DMSO (D1435, ≥99.5%), crystal violet (C0775, dye content ≥90%), and methylcellulose (#M0387) were purchased from Sigma-Aldrich (St. Louis, MO, United States); remdesivir (GS-5734) (S8932, 99.3%, HPLC) and cyclosporine A (cyclosporine) (S2286, 99.6%, HPLC) from Selleckchem (Houston, TX, United States); Goat anti-human IgG-Alexa Fluor 488 (A11013), Hochest 333258 (H3569), and DAPI (D1306) from Invitrogen (Thermo Fisher Scientific, Waltham, MA, United States); and 10% formaldehyde solution from Marcon™ Chemicals (#H121-08). The antibody against nucleocapsid (N) protein of HCoV-OC43 (Mab9013) was purchased from Merck Millipore (Burlington, MA, United States), and fluorescein isothiocyanate (FITC)-conjugated anti-mouse immunoglobulin (#55499) from MP Biomedicals (Irvine, CA, United States). Anti-SARS-CoV-2 N protein antibodies were provided by Dr An-Suei Yang of the Genomics Research Center, Academia Sinica.

Hsu H.Y., Yang C.W., Lee Y.Z., Lin Y.L., Chang S.Y., Yang R.B., Liang J.J., Chao T.L., Liao C.C., Kao H.C., Wu S.H., Chang J.Y., Sytwu H.K., Chen C.T, & Lee S.J. (2021). Remdesivir and Cyclosporine Synergistically Inhibit the Human Coronaviruses OC43 and SARS-CoV-2. Frontiers in Pharmacology, 12, 706901.

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