Absolute counts of CD4+ and CD8+ T-cells were determined in fresh whole blood by using an Epic XL-MCL flow cytometer (Beckman-Coulter, Brea, CA) according to the manufacturer's instructions. The plasma HIV-1 RNA concentration was measured by using quantitative PCR (Cobas Ampliprep/Cobas TaqMan HIV-1 test; Roche Molecular Systems, Basel, Switzerland) according to the manufacturer's protocol. The detection limit for this assay was 50 HIV-1 RNA copies/mL. Hepatitis C virus (HCV) RNA was determined using an available PCR procedure kit (Cobas Amplicor; Roche Diagnostics, Barcelona, Spain) with a detection limit of 10 HCV-RNA copies/ml. Plasma samples were tested for anti-HCV antibodies using HCV-ELISA (Siemens Healthcare Diagnosis, Deerfield, IL, USA). HCV genotype was determined using a reverse-hybridization assay (InnoLIPA HCV II; Innogenetics, Barcelona, Spain).
Epic xl mcl flow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The Epic XL-MCL is a flow cytometer designed for high-throughput analysis of cells. It features a compact design and can process multiple samples simultaneously. The instrument utilizes advanced optics and detection technology to provide accurate and reliable cell analysis data.
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Lab products found in correlation
3 protocols using epic xl mcl flow cytometer
1
Comprehensive T-cell and Viral Load Monitoring
2
Immunological and Viral Biomarker Measurements
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CD4 T-cell counts were determined in fresh whole blood using an Epic XL-MCL flow cytometer (Beckman-Coulter, Brea, CA, USA) according to the manufacturer’s instructions. Plasma HIV-1 RNA concentration was measured using quantitative polymerase chain reaction (COBAS Ampliprep/COBAS Taqman HIV-1 test, Roche Molecular Systems, Basel, Switzerland) according to the manufacturer’s protocol. The detection limit for this assay was 20 HIV RNA copies per milliliter.
High-sensitive C-reactive protein (hsCRP) and β2-microglobulin were determined with an immunoturbidimetric assay using COBAS 701 (Roche Diagnostics, GmbH, Mannheim, Germany).d -dimer levels were determined using an automated latex enhanced immunoassay (HemosIL, d -Dimer HS 500, Instrumentation Laboratory) in plasma samples stored at −20°C.
High-sensitive C-reactive protein (hsCRP) and β2-microglobulin were determined with an immunoturbidimetric assay using COBAS 701 (Roche Diagnostics, GmbH, Mannheim, Germany).
3
Immunological and Virological Profiling of HIV/HCV Coinfection
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Absolute counts of CD4+ and CD8+ T-cells were determined in fresh whole blood by using an Epic XL-MCL flow cytometer (Beckman-Coulter, Brea, California) according to the manufacturers' instructions. Plasma HIV-1 RNA concentration was measured by quantitative polymerase chain reaction (COBAS Ampliprep/COBAS Taqman HIV-1 test, Roche Molecular Systems, Basel, Switzerland) according to the manufacturer's protocol. The detection limit for this assay was 50 HIV-1-RNA copies/mL. Hepatitis C virus (HCV) RNA was determined using an available PCR procedure Kit (COBAS Amplicor, Roche Diagnosis, Barcelona, Spain) with a detection limit of 10 IU/mL.
Total DNA was extracted from peripheral blood mononuclear cells (PBMCs) using the MagNa Pure LC DNA isolation kit. HLA-B group alleles were genotyped using a reverse sequence-specific oligonucleotide bound to a fluorescently coded microsphere system (LABType SSO, RSSO1B, One Lambda, Canoga Park, CA), following manufacturer's instructions.
Total DNA was extracted from peripheral blood mononuclear cells (PBMCs) using the MagNa Pure LC DNA isolation kit. HLA-B group alleles were genotyped using a reverse sequence-specific oligonucleotide bound to a fluorescently coded microsphere system (LABType SSO, RSSO1B, One Lambda, Canoga Park, CA), following manufacturer's instructions.
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