The following antibodies were used in the study: anti-BAF155 (Abcam, Cambridge, UK, ab172638, IP and Western blotting), anti-BAF170 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA sc166537, Western blotting), anti-BRD9 (Active Motif, Carlsbad, CA, USA 61537, IP and Western blotting), anti-BRD9 (Bethyl Laboratories, Inc., Montgomery, TX, USA, A303-781A, immunocytochemistry), anti-BRD9 (ThermoFisher, customized using porcine BRD9 peptide sequence, IP and Western blotting), anti-BRG1 (Abcam, ab110641, IP and Western blotting), anti-GLTSCR1 (Santa Cruz, sc51508, IP, Western blotting, and immunocytochemistry), anti-SNF5 (Abcam, ab88589, immunocytochemistry), mouse IgG (Abcam, ab6708, IP and Western blotting), rabbit IgG (Abcam, ab171870, IP and Western blotting), mouse IgGκ BP-HRP (Santa Cruz, sc516102, Western blotting), goat anti-rabbit IgG (H + L) (ThermoFisher (Invitrogen), A27036, Western blotting), goat anti-rabbit IgG-TRITC (Sigma, T6778, immunocytochemistry), and anti-rabbit IgG (whole molecule)-FITC (Sigma, F0382).
Anti baf155
Manufactured by Abcam
Sourced in United Kingdom
Anti-BAF155 is a primary antibody that recognizes the BAF155 protein, a subunit of the SWI/SNF chromatin remodeling complex. The antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the expression and localization of the BAF155 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti brd9, by Fortis Life Sciences (1 mentions)
Mouse iggκ bp hrp, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg tritc, by Merck Group (1 mentions)
Anti brd9, by Active Motif (1 mentions)
Anti brg1, by Abcam (1 mentions)
Anti β tubulin, by Merck Group (1 mentions)
Anti brg1 h88, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh 14c10, by Cell Signaling Technology (1 mentions)
Protein g sepharose beads, by Merck Group (1 mentions)
Sds page sample buffer, by Merck Group (1 mentions)
4 protocols using anti baf155
1
Antibody Validation for Chromatin Remodeling
2
Western Blot Antibody Characterization
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For Western blot analyses, the following antibodies were used: anti-AGO2 (11A9, Millipore), anti-Histone H3 antibody (FL-136, Santa Cruz), anti-BAF155 (Abcam), antibeta Tubulin (Sigma).
3
Western Blot Antibody Analysis
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For western blot analyses the following antibodies were used: anti-AGO1 (4B8, Ascenion), anti-AGO2 (11A9, Ascenion), anti-GAPDH (14C10, Cell Signaling technology), anti-histone H1 + core proteins (F152.C25.WJJ, Millipore) anti-β-tubulinI (SAP.4G5, Sigma), anti-BRG1 (H-88, Santa Cruz), anti-BAF155 (Abcam), anti-INI1 (Bethyl Laboratoties), goat-anti mouse and anti-rabbit IgG–HRP conjugated (Bio-Rad), anti-rat IgG–HRP conjugated (Jackson).
4
Immunoprecipitation of AGO1/2 and BAF155
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Antibodies anti-AGO1 (4B8, Ascenion); anti-AGO2 (11A9, Ascenion); anti-BAF155 (Abcam)) and isotype-matched IgG (as mock IP) were coupled to protein-G-sepharose beads (Sigma).
Whole-cell lysate (40-80 × 106 cells) or nuclear fraction (60-160 × 106 cells) were incubated overnight at 4°C with antibodies-coupled beads. IP were washed once with IP-buffer and three times with IP-wash buffer (50 mM Tris–HCl, pH 7.5; 300 mM NaCl; 5 mM MgCl2; and 0.05% nonidet P-40). Proteins were eluted from beads by boiling for 5 min in SDS-PAGE sample buffer (Sigma).
Whole-cell lysate (40-80 × 106 cells) or nuclear fraction (60-160 × 106 cells) were incubated overnight at 4°C with antibodies-coupled beads. IP were washed once with IP-buffer and three times with IP-wash buffer (50 mM Tris–HCl, pH 7.5; 300 mM NaCl; 5 mM MgCl2; and 0.05% nonidet P-40). Proteins were eluted from beads by boiling for 5 min in SDS-PAGE sample buffer (Sigma).
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