Log In Sign up
The largest database of trusted experimental protocols

Idh1 r132h mutant protein clone h09

Manufactured by Dianova
Visit Supplier
Sourced in Switzerland, Germany

The IDH1-R132H mutant protein (clone H09) is a recombinant protein that contains the R132H mutation in the isocitrate dehydrogenase 1 (IDH1) gene. This mutation is commonly found in certain types of cancer cells. The protein is produced in a controlled laboratory setting and can be used for research purposes, such as studying the effects of the IDH1-R132H mutation on cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Bond 3, by Leica (1 mentions) P53 clone do 7, by Agilent Technologies (1 mentions) Atrx hpa001906, by Merck Group (1 mentions) Ultraview universal dab detection, by Roche (1 mentions) Benchmark xt autostainer, by Roche (1 mentions)

Close spelling variants of this product

IDH1 R132H (clone H09, IDH1 R132H H09 clone

2 protocols using idh1 r132h mutant protein clone h09

1

Detection of IDH1-R132H Mutation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining for IDH1-R132H mutant protein (clone H09; Dianova, Switzerland) was performed on 4 μm-thick formalin-fixed paraffin-embedded tissue sections in the automated BOND-III (Leica Biosystems, Germany). For patients < 55 years old, Sanger sequencing was performed to detect rare IDH1/2 mutations, as described elsewhere.9 (link)
Sourty B., Basset L., Fontaine A., Garcion E, & Rousseau A. (2024). Chromothripsis is rare in IDH-mutant gliomas compared to IDH-wild-type glioblastomas whereas whole-genome duplication is equally frequent in both tumor types. Neuro-Oncology Advances, 6(1), vdae059.
+ Open protocol
+ Expand
2

Immunohistochemistry and FISH Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed on formalin-fixed, paraffin embedded tissue sections at the UCSF Immunohistochemistry Laboratory and the UCSF Neuropathology BTRC Biomarkers Laboratory. Primary antibodies used were as follows: histone H3-K27M mutant protein (ABE419, EMD Millipore, Billerica, MA, 1:500 dilution), IDH1-R132H mutant protein (clone H09, DiaNova, Germany, 1:500 dilution), ATRX (HPA001906, Sigma, St Louis, MO, 1:100 dilution), p53 (clone DO-7, Dako, Glostrup, Denmark, 1:00 dilution), BRAF-V600E mutant protein (clone VE1, Ventana, Tucson, AZ). All staining was performed in Ventana or Leica Bond automated staining processors. Specifically, the histone H3-K27M immunostaining was run in a Ventana Benchmark XT autostainer using CC1 antigen retrieval buffer for 30 minutes at 95°C, incubation with 1:500 dilution of primary antibody for 32 minutes, and Ventana ultraView Universal DAB detection. Dual-color FISH for EGFR and CEP7 or PTEN and CEP10 was performed on 5-micron thick FFPE whole sections as previously described (17 (link)).
Solomon D.A., Wood M.D., Tihan T., Bollen A.W., Gupta N., Phillips J.J, & Perry A. (2015). Diffuse Midline Gliomas with Histone H3‐K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic Alterations. Brain Pathology, 26(5), 569-580.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!