All cells were maintained at 37°C with 5% CO2. HEK293T were grown in DMEM (Corning) supplemented with 10% fetal bovine serum (FBS, Corning), Penicillin-Streptomycin (100 mg/ml, Millipore) and L-glutamine (2 mM, Corning). K562, K562-dCAS9-KRAB, OVCAR8, Kuramochi, OVISE, OVCAR4, PEO1 were grown in RPMI-1640 (Invitrogen) supplemented with 10% fetal bovine serum (FBS, Corning), Penicillin-Streptomycin (100 mg/ml, Millipore) and 1% GlutaMax (Millipore). OVCAR3 were grown in RPMI-1640 (Invitrogen) supplemented with 20% fetal bovine serum (FBS, Corning), Penicillin-Streptomycin (100 mg/ml, Millipore) and 1% GlutaMax (Millipore). OV90, CAOV3 and OAW28 were grown in DMEM/F12 (Invitrogen) supplemented with 10% fetal bovine serum (FBS, Corning), Penicillin-Streptomycin (100 mg/ml, Millipore) and 1% GlutaMax (Millipore). All cell lines were routinely tested for Mycoplasma and if not noted elsewhere were obtained from American Tissue Type Collection (ATCC). Whenever thawed, cells were passaged at least three times before being used in experiments.
Penicillin streptomycin
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, France, Switzerland, Japan, Canada, Australia, Austria, Sao Tome and Principe, Spain, Macao, Israel, Brazil, Poland, Ireland, Belgium, Denmark, Portugal, India, Sweden, Norway, Mexico, Czechia, Netherlands, Senegal
Penicillin/streptomycin is a commonly used antibiotic mixture for cell culture applications. It provides broad-spectrum antimicrobial activity to prevent bacterial contamination in cell culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1601 mentions)
Fetal bovine serum (fbs), by Merck Group (1172 mentions)
L glutamine, by Merck Group (1014 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (755 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (589 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (434 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (277 mentions)
L glutamine, by Thermo Fisher Scientific (270 mentions)
Glutamax, by Thermo Fisher Scientific (242 mentions)
Rpmi 1640, by Thermo Fisher Scientific (233 mentions)
Rpmi 1640, by Merck Group (219 mentions)
Rpmi 1640 medium, by Merck Group (210 mentions)
Insulin, by Merck Group (206 mentions)
Dmem f12, by Thermo Fisher Scientific (190 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (188 mentions)
Sodium pyruvate, by Merck Group (160 mentions)
Glutamine, by Merck Group (158 mentions)
Hepes, by Merck Group (152 mentions)
Trypsin edta, by Merck Group (152 mentions)
Fetal calf serum (fcs), by Merck Group (149 mentions)
6 987 protocols using penicillin streptomycin
1
Cell Culture Maintenance Protocols for Diverse Cell Lines
2
Establishment and Maintenance of Mesothelioma Cell Lines
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Primary MPM cultures were established from surgical specimens and maintained with serum and without serum as previously described [25 (link),49 (link)]. MPM cells-SPC111, SPC212, ZL34 and ZL55, were established in our laboratory [50 (link)] and were maintained in DMEM:F12 (Ham) medium (Sigma-Aldrich, St. Louis, MO, USA) with 15% foetal calf serum (FCS, Invitrogen/GIBCO) and 1% penicillin/streptomycin (Sigma-Aldrich); Mero- 14, Mero-41, Mero-48, Mero-82, Mero-83, Mero-84 and Mero-95 were maintained in DMEM:F10 (Ham) medium (Sigma-Aldrich) with 15% FCS and 1% penicillin/streptomycin; ONE-58, ACC-Meso-1 and ACC-Meso-4 were maintained in RPMI-1640 (Sigma-Aldrich) with 15% FCS and 1% penicillin/streptomycin; MSTO-211H, H2052, H2452, H226, NO36 and H596 cells were maintained in RPMI-1640 with 10% FCS and 1% penicillin/streptomycin. ZL55SPT and SDM103T2 primary cells were grown in serum-free medium [25 (link)]. Normal mesothelial cells NP3, SDM77, SDM85, SDM104, SDM58 and SDM71 were cultured as previously described [51 (link)]. NP3 cells were also grown in serum-free medium. To induce growth arrest, cells were treated either with Hedgehog signaling inhibitor (HhAntag) [25 (link)] or with dual PI3K/mTOR inhibitor (NVP-BEZ235, Novartis, Switzerland) [26 (link)], as previously described.
3
Cell Line Maintenance Protocols
4
Cell Culture Conditions and Sources
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TIG-3 cells (11 (link), 38 (link), 52 (link)) and IMR-90 cells were obtained from the Japanese Cancer Research Resources Bank and American Type Culture Collection, respectively. TIG-3 cells, IMR-90, and IMR-90/ER:H-RasV12 cells (52 (link)) were cultured in Dulbecco’s Modified Eagle’s (DME) medium (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (Sigma-Aldrich) at physiological oxygen conditions (92% N2, 5% CO2, and 3% O2) at 37 °C. RPE-1/hTERT cells (39 ) and HEK-293T cells (52 (link)) were cultured in DME medium (Nacalai Tesque) supplemented with 10% FBS and penicillin/streptomycin (Sigma-Aldrich) in a 5% CO2 incubator at 37 °C. SVts8 cells (24 (link)) were cultured in DME medium (Nacalai Tesque) supplemented with 10% FBS and penicillin/streptomycin (Sigma-Aldrich) in a 5% CO2 incubator at 34 °C. MEFs were generated from CD-1 mice as previously described (53 (link)) and then cultured in DME medium (Nacalai Tesque) supplemented with 10% FBS and penicillin/streptomycin (Sigma-Aldrich) at physiological oxygen conditions (92% N2, 5% CO2, and 3% O2) at 37 °C. All cell lines used were negative for mycoplasma.
5
Breast Cancer Cell Line Cultivation
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BT474, BT549, Hs578T, MCF7, MDA-MB-231, MDA-MB-468, SK-BR-3, and T-47D, cells were purchased from the American Type Culture Collection (ATCC). HMLE cells were a kind gift from Robert Weinberg, Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology. BT474, BT549, MDA-MB-231, MDA-MB-468, SK-BR-3, and T-47D were cultured in RPMI 1640 (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) (Biochrom) and 1% penicillin-streptomycin (Sigma-Aldrich). BT549 cells were grown in the presence of 0.001 mg/ml insulin (Sigma-Aldrich) and T-47D were grown in the presence of 0.006 mg/ml insulin (Sigma-Aldrich). Hs578T were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich) supplemented with 10% FBS, 1% penicillin-streptomycin, and 0.01 mg/ml insulin. MCF7 were cultivated in Minimum Essential Medium Eagle (MEM; Sigma-Aldrich) supplemented with 10% FBS, 1% penicillin-streptomycin, and 0.01 mg/ml insulin. HMLE cells were grown in a 1:1 mixture of MEBM (Lonza) with DMEM/F12 (Sigma-Aldrich) supplemented with 10 ng/ml EGF, 0.5 μg/ml hydrocortisone, 0.01 mg/ml insulin, and 1% penicillin-streptomycin. All cell lines were incubated in a 5% CO2 humidified incubator at 37°C.
6
Cell Culture Conditions for Cancer Research
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B16.F10 and B16.OVA cells (CRL-6475, ATCC) were maintained in DMEM with 10% fetal bovine serum (both Life Technologies) and 1% penicillin-streptomycin (Sigma-Aldrich). Lung tumour cells were maintained in HAMS-F12 with 10% fetal bovine serum (both Life Technologies) and 1% penicillin-streptomycin (Sigma-Aldrich). FRCs were maintained in RPMI with 10% fetal bovine serum, 10 mM HEPES (all Life Technologies), 1% penicillin-streptomycin, 15 μM β-mercaptoethanol (both Sigma-Aldrich). CAFs and normal fibroblasts were maintained in DMEM with 1.5 g L−1 NaHCO3, 10% fetal bovine serum (both Life Technologies) and 1% penicillin-streptomycin (Sigma-Aldrich). T cells were maintained in IMDM with 5% fetal bovine serum (both Life Technologies), 1% pencillin-streptomycin and 15 μM β-mercaptoethanol (both Sigma-Aldrich). All cells in culture were routinely tested for mycoplasma contamination (MycoAlert Detection Kit, Lonza).
7
Cell Line Maintenance Protocols
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MCF-10A and MCF-10A MYC-ER cells were a gift from Senthil K. Muthuswamy (Beth Israel Deaconess Medical Center) and were maintained in DMEM/F12 (Gibco) supplemented with 5% horse serum (GIBCO), 1% penicillin streptomycin (Sigma), 20 ng/mL EGF (Peprotech), 2 ug/mL hydrocortisone 0.5 ug/mL (Sigma), 100 ng/mL cholera toxin (Sigma), and 10 ug/mL insulin (Sigma).121 (link) HCC1806 cells, a gift from Edison Liu (Jackson Laboratory), were maintained in DMEM (Gibco) supplemented with 15% FBS and 1% penicillin streptomycin (Sigma). HEK293T cells (ATCC), were maintained in DMEM (Gibco) supplemented with 10% FBS, 1% penicillin streptomycin (Sigma). MDA-MB231 GFP-luciferase rTTA3-puro7 (link) were maintained in DMEM (Gibco) supplemented with 20% FBS, 1% penicillin streptomycin (Sigma). All cell lines were grown at 37°C under a humidified atmosphere with 5% CO2. Cells routinely tested negative for mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (Lonza). Cell aliquots from early passages were used.
8
Culturing Leukemia Cell Lines for Research
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Leukemia cell lines were obtained from the Cell Culture Facility of CEINGE - Advanced Biotechnologies (Naples, Italy). RS4;11, SEM, and MV4-11 harbor the t(4;11) chromosomal rearrangement and express endogenous MLL-AF4 chimera. The RS4;11 acute lymphoblastic leukemia cells were grown at 37 °C, 5% CO2, in minimum essential medium (MEM) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% FBS and 10 mL/L penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA); SEM acute lymphoblastic leukemia cells were grown at 37 °C, 5% CO2, in Iscove’s MDM (Sigma-Aldrich) supplemented with 10% FBS and 10 mL/L penicillin/streptomycin; MV4-11 acute monocytic leukemia cells and 697 acute lymphoblastic leukemia cells were grown in RPMI (Sigma-Aldrich) supplemented with 20% FBS and 10 mL/L penicillin/streptomycin.
9
Adaptation of Pancreatic Cancer Cells to Acidic pH
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All experiments were performed with mycoplasma‐free cells. Panc02 mouse pancreatic cancer cells (RRID: CVCL_D627), a gift from A. Trauzold, University of Kiel, Germany, were cultured in DMEM (Gibco Life Technologies) with 10% FBS (Sigma, F9665‐500ML) and 1% Penicillin/Streptomycin (Sigma, P0781‐100ML) at 5% CO2/37°C. Panc02 cells were adapted to growth at pH 6.7 by decreasing the pH of the culture medium by ~0.2 pH units weekly. Panc‐1 cells (RRID CVCL_0480) and AsPC‐1 cells (RRID CVCL_0152) were obtained from ATCC (Manassas, VA, USA) and were cultured in AsPC‐1 and Panc‐1 cells were cultured in DMEM (10569010 and 41966029, respectively, Gibco Life Technologies) with 10% FBS (Sigma, F9665‐500ML) and 1% Penicillin/Streptomycin (Sigma, P0781‐100ML) at 5% CO2/37°C and acid‐adapted by transfer to pH 6.7 medium followed by a month of growth under these conditions. Culture media were pH‐adjusted by titration with hydrochloric acid and measured at 5% CO2. Once pH 6.7 was reached, cells were cultured for maximally 8 weeks. HUVECs were cultured in Endothelial Cell Growth Medium (C‐22210, PromoCell) with Growth Medium Supplement Mix (C‐39215, PromoCell) and 1% Penicillin/Streptomycin (Sigma, P0781‐100ML) at 5% CO2/37°C.
10
Culturing Human Leukemia Cell Lines
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Human erythroleukemia cell lines, YN-1 (Endo et al. 1993 ) and K562 (Dorfman et al. 1992 ); human T cell leukemia cell line, Jurkat; human pre-B cell leukemia cell line, Nalm6; and human monocytoid cell line, U937 were cultured in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS; Biowest, Miami, FL, USA) and 1% penicillin/streptomycin (Sigma-Aldrich, St Louis, MO, USA). U937 cells stably expressing pGL4.20 (GATA-2 +9.9/1S; described below) were cultured in RPMI-1640 containing 10% FBS, 1% penicillin/streptomycin, and 1 µg/ml puromycin (Sigma-Aldrich). K562 and U937 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and the Cell Resource Center for Biomedical Research at Tohoku University (http://www2.idac.tohoku.ac.jp/dep/ccr/), respectively. The human embryonic kidney cell line, HEK293T and retrovirus packaging cells (PLAT-F and PLAT-GP) were maintained in Dulbecco modified Eagle medium containing 10% FBS (Biowest) and 1% penicillin/streptomycin (Sigma-Aldrich) (Kamata et al. 2014) .
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