Matrigel

Manufactured by BD

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Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.

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Lab products found in correlation

Transwell chamber, by Corning (1085 mentions) Transwell chamber, by BD (324 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (315 mentions) Crystal violet, by Merck Group (313 mentions) Transwell insert, by Corning (279 mentions) Inverted microscope, by Olympus (236 mentions) Crystal violet, by Beyotime (229 mentions) Transwell chamber, by Merck Group (225 mentions) Transwell plate, by Corning (191 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (179 mentions) Matrigel, by Corning (164 mentions) 24 well transwell chamber, by Corning (143 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (136 mentions) Mtesr1 medium, by STEMCELL (131 mentions) Glutamax, by Thermo Fisher Scientific (123 mentions) Matrigel, by Thermo Fisher Scientific (117 mentions) Dmem f12, by Thermo Fisher Scientific (105 mentions) Matrigel, by Merck Group (97 mentions) Upper chamber, by Corning (97 mentions) Transwell insert, by BD (93 mentions)

Close spelling variants of this product

Matrigel Basement Membrane Matrix (766 citations) Matrigel-coated plates (74 citations) BD Matrigel Matrix High Concentration (15 citations) Matrigel transwell chamber (10 citations) Liquid Matrigel (8 citations) Matrigel for invasion assay (7 citations) Matrigel invasion assay kit (5 citations) Matrigel matrix glue (5 citations) Matrigel-coated membrane inserts (4 citations) Ice-cold matrigel (4 citations)

17 210 protocols using matrigel

Macrophage Activation in Matrigel Plugs

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Matrigel plug implanted in C57BL/6 mice was used for macrophage in vivo activation. To induce activated macrophages in mice, 0.25 mL Matrigel (BD Biosciences) containing rmM-CSF (100 ng/mL)+PBS was used for naive macrophage differentiation, Matrigel containing rmM-CSF+LPS (10ng/mL) + IFN-γ (20ng/mL) for M1, Matrigel containing rmM-CSF+IL-4 (20 ng/mL) for M2a, Matrigel containing rmM-CSF+immune-complexes (10 μg/mL)+LPS (50 ng/mL) for M2b, Matrigel containing rmM-CSF+IL-10 (50ng/mL) for M2c, and Matrigel containing PBS only for control group. All procedures were completed on ice. C57BL/6 mice were anesthetized with isoflurane, then Matrigel mixture was subcutaneously injected into the inguinal areas of mice.
Five days after plug implantation, mice were euthanized by CO₂ asphyxiation for Matrigel plug excision. For ex vivo live-cell imaging, Matrigel plugs were labeled by MitoTracker Red CMXRos and Hoechst 33342. Then they were placed in the micro-incubator system on the confocal microscope for further observation. MitoTracker images were collected at 561 nm excitation, and Hoechst images were collected at 405 nm excitation. For immunofluorescence staining, Matrigel plugs were OCT-embedded and sectioned for further examination.

Li Y., He Y., Miao K., Zheng Y., Deng C, & Liu T.M. (2020). Imaging of macrophage mitochondria dynamics in vivo reveals cellular activation phenotype for diagnosis. Theranostics, 10(7), 2897-2917.

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Angiogenic Potential of hASC-Conditioned Media

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Conditioned basal media was prepared by culturing 5 x 10⁵ hASCs within 30-μl gel volume of various SHIELD formulations in EBM-2 basal media (Lonza) for two days. In parallel, human microvascular endothelial cells (hMVECs) were cultured in complete growth media (EGM-2 BulletKit, Lonza). Bioactivity of the hASC-conditioned medium was assessed using a traditional Matrigel sandwich assay.^{[19 (link), 24 ]} In brief, hMVECs (1 x 10⁴ cells) were seeded on top of growth factor reduced Matrigel (BD Biosciences, 200 μl in a 48-well), allowed to attach for 4 h, and covered with another layer of Matrigel (200 μl) to create a Matrigel sandwich. The Matrigel sandwich was immersed in unconditioned basal media or hASC-conditioned media. Following incubation at 37 °C for two days, the Matrigel sandwich was fixed in 4% paraformaldehyde and 2% glutaraldehyde, and permeabilized with 0.25% Triton X-100 solution in PBS. The cells were stained with rhodamine phalloidin and DAPI, and analyzed using confocal microscropy as described above. Number of junctions, total tubule length, and loop perimeter were quantified for each image using ImageJ software.^{[19 (link), 24 ]}

Cai L., Dewi R.E., Goldstone A.B., Cohen J.E., Steele A.N., Woo Y.J, & Heilshorn S.C. (2016). Regulating Stem Cell Secretome Using Injectable Hydrogels with In situ Network Formation. Advanced healthcare materials, 5(21), 2758-2764.

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Oncosphere Formation of Lung Epithelial Cells

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CD24⁺/ITGB4⁺/NOTCH3^hi cells were resuspended in BEGM without hydrocortisone (Lonza, Walkersville, MD) containing 10 ng/mL of keratinocyte growth factor (PeproTech, Rocky Hill, NJ, USA), 5% charcoal stripped fetal bovine serum and 50% Matrigel (BD Biosciences, San Jose, CA, USA), and plated on Matrigel-coated tissue culture wells topped with BEGM. Medium was changed twice weekly. Self-renewal was assessed in oncospheres released from Matrigel using Cell Recovery Solution (BD Biosciences). Cells were disassociated into single cells by triturating with StemPro® Accutase (Invitrogen, life technology) and assessed for oncosphere size and number 2 weeks after being plated in 96-well Matrigel-coated plates. In some experiments, total lung epithelial cells isolated from NTg, Ect2^fl/fl, K,P and K,P,Ect2^fl/fl mice were plated as described above and infected with Ad-Cre (MOI 50). Cells were released from Matrigel culture using BD Cell Recovery Solution (BD Biosciences) and total RNA and DNA were isolated for analysis.

Justilien V., Ali S.A., Jamieson L., Yin N., Cox A.D., Der C.J., Murray N.R, & Fields A.P. (2017). Ect2-dependent rRNA synthesis is required for KRAS/TP53-driven lung adenocarcinoma. Cancer cell, 31(2), 256-269.

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Transplantation of Neural Spheroids

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Cells were prepared in hanging drops with Matrigel (BD Biosciences 354234, San Jose CA), at a concentration of 4 x 10 6 cells/ ml with 0.5% Matrigel for the SHSY5Y cell line, a concentration of 8 x 10^6 cells / ml with 0.5% Matrigel for the NB-1643 cell line and at 8 x 10^6 cells / ml with 1% Matrigel for the SK-N-BE (2) cell line. The hanging drops were prepared 40-48 hours in advance of use and cut using a pulled glass needle to the size of 1 chick somite (approximately 150 um square; 300-500 cells) for transplantation. Using a P-2 pipette, the transplant was placed near the chick embryo and manipulated using a tungsten needle into the dorsal neural tube of the embryo behind the last formed pair of somites. Embryos were left for 1 hour at room temperature to settle before being re-incubated for an additional 48 hours. Afterwards, embryos were harvested, fixed in 4% PFA (from where) and washed in PBS.

Kasemeier-Kulesa J.C., Spengler J.A., Muolo C.E., Morrison J.A., Woolley T.E., Schnell S, & Kulesa P.M. (2021). The embryonic trunk neural crest microenvironment regulates the plasticity and invasion of human neuroblastoma via TrkB signaling. Developmental biology, 480.

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Endothelial Cell Tube Formation Assay

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The tube formation assay was performed as described previously [40 (link)]. Matrigel (Becton Dickinson, Bedford, MA, USA) was diluted with cold serum-free M199 media to 10 mg/mL. The diluted Matrigel solution was added to 96-well plates (70 μL per well) and allowed to form a gel at 37 °C for 1 h. Cell suspensions (3 × 10⁴ cells/70 μL per well) in M199 media containing 10% FBS were plated on Matrigel-coated wells and incubated for 6–8 h at 37 °C in 5% CO₂. After incubation, the endothelial tubes were observed and photographed using a microscope with a digital images system.

Lin S.W., Huang S.C., Kuo H.M., Chen C.H., Ma Y.L., Chu T.H., Bee Y.S., Wang E.M., Wu C.Y., Sung P.J., Wen Z.H., Wu D.C., Sheu J.H, & Tai M.H. (2015). Coral-Derived Compound WA-25 Inhibits Angiogenesis by Attenuating the VEGF/VEGFR2 Signaling Pathway. Marine Drugs, 13(2), 861-878.

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Transwell Assay for Cell Migration and Invasion

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For cell migration capacity analysis, 2 × 10⁵ transfected cells were suspended in serum-free DMEM (200 μL) and added to the apical chamber of a Transwell uncoated with Matrigel (356,234, BD Bioscience, San Jose, CA, USA).
For cell invasion analysis, Matrigel (356,234, BD Bioscience) was diluted (1:10) in serum-free DMEM and diluted Matrigel (100 μL) was added to the apical chamber of the Transwell and incubated for more than 30 min. Then 2 × 10⁵ transfected cells were then seeded into the apical chamber coated with Matrigel.
The basolateral chambers were both spiked with DMEM containing 10% FBS (600 μL) and then incubated at 37 °C in an incubator for 24 h. Cells were then fixed with 4% paraformaldehyde for 15 min and stained with crystal violet (0.1%) for 15 min. The stained positive cells were then observed using an inverted light microscope (CarlZeiss, Germany) and photographed for imaging, with ImageJ software used for positive cell counting.

Wang X., Zhang M., Jiang L., Fang X, & Zhang T. (2022). Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis. Reproductive Biology and Endocrinology : RB&E, 20, 77.

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Cell Invasion and Migration Assay

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For invasion assays, Matrigel (BD company) was maintained at 4°C overnight for dissolution before dilution with cold serum-free RPMI 1640 (Matrigel: RPMI 1640 = 1:8). Basement membrane matrix Boyden chambers (8-mm pore size) were purchased from BD Company and 100 ul Matrigel was added to each upper chamber. Resuspended cells in serum-free RPMI-1640 were adjusted to a cell density of 1–5 × 105/ml, with total cells being 2–10 × 10⁴ per well (H1299: 5 × 10⁴ per well; H838: 10 × 10⁴ per well). 200 μl of RPMI-1640 with cells was added into the upper chamber and 600 μl of 20% FBS-containing RPMI-1640 was added into the lower chamber. Cells were incubated for 48 h at 37°C, then fixed, stained, and counted. Five fields are randomly selected for counting the migrated cell numbers. For invasion assays, the only difference was that no Matrigel was included in the basement membrane matrix Boyden chambers.

Zhao H., Wang Y., Wu X., Zeng X., Lin B., Hu S., Zhang S., Li Y., Zhou Z., Zhou Y., Du C., Beer D.G., Bai S, & Chen G. (2022). FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKɑ signaling in lung adenocarcinoma. Bioengineered, 13(5), 13312-13327.

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Myocardial Infarction and Cell Therapy

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A total of 39 female Wistar rats (8-10 weeks) weighing 200 to 250 g were used for in vivo procedures. MI was induced by left coronary artery (LCA) ligation as previously described 30 in 34 female Wistar rats. Shamoperated (SHAM, n = 5) animals underwent the same procedure, but the coronary ligature was left untied. Twenty-nine percent of the animals (10 out of 34) died during MI induction or in the 24 h after surgery. Four weeks after MI induction, 3 out of 24 infarcted animals that survived had left ventricular EF (LVEF) above 50% and were excluded. The large initial number of animals was chosen to provide at least five to six rats per group for each of the measurements, as a high mortality rate is expected for rats subjected to MI (30-40%). Four weeks post-MI, rats were subjected to another thoracotomy and received an intramyocardial injection of 100 µl of 2 × 10 6 IGF-1-ADSCs in Matrigel (BD Biosciences) (n = 7), 100 µl of 2 × 10 6 ADSCs in Matrigel (BD Biosciences) (n = 7), or 100 µl of vehicle (PBS/Matrigel solution) (n = 7). Six weeks after treatment, the animals were subjected to functional evaluation and then sacrificed by cervical dislocation under anesthesia. The hearts were excised and processed for histology (see below) and to track the injected cells by PCR detection of the Y chromosome.

Bagno L.L., Carvalho D., Mesquita F., Louzada R.A., Andrade B., Kasai-Brunswick T.H., Lago V.M., Suhet G., Cipitelli D., Werneck-de-Castro J.P, & Campos-de-Carvalho A.C. (2016). Sustained IGF-1 Secretion by Adipose-Derived Stem Cells Improves Infarcted Heart Function. Cell transplantation, 25(9).

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Transwell Invasion and Migration Assay

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Transwell chambers (Corning, New York, USA) coated with or without Matrigel (BD
Biosciences, San Jose, USA) were used to conduct invasion (with Matrigel) and migration
(without Matrigel) experiments, respectively. The cells (2×10 ⁵) cultured in CM
or MR medium were added to the upper chamber. The lower chamber was filled with RPMI-1640
medium (Gibco) containing 10% fetal calf serum (Gibco). The culture system was incubated
in an incubator for 24 h, the upper layer of the medium was removed, and the Transwell
membrane was completely immersed in methanol after washing with phosphate-buffered saline
(PBS) and fixed at room temperature for half an hour. Subsequently, the methanol was
discarded, and the chambers were stained with crystal violet (Zhongshan Bio, Beijing,
China) for 15 min. Finally, the stained chambers were placed under a confocal fluorescence
microscope (Leica, Wetzlar, Germany), and 5 fields were randomly selected to count the
number of penetrating cells in the lower layer.

Li Y., Liu C., Xin L., Liu C., Cao J., Yue Z., Sheng J., Yuan Y., Zhou Q, & Liu Z. (2023). Upregulation of E-cadherin by the combination of methionine restriction and HDAC2 intervention for inhibiting gastric carcinoma metastasis: Synergetic MR and HDAC2 intervention in GC metastasis. Acta Biochimica et Biophysica Sinica, 56(1), 62-70.

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Matrigel-Coated Cell Invasion Assay

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The filters (8 μm pore; Coster, Cambridge, MA) were coated with 100 μL Matrigel (BD Bioscience, San Jose, CA, USA), which was diluted in 1:2 with serum-free 1640 medium, and incubated at 37^ºC in 5% CO₂ for 30 mins for Matrigel 2×10⁴ lentivirally infected CRC cells in 100 μL of culture medium were seeded in the upper Matrigel chamber and a 700 μL 0.2% FBS conditional medium was added in the lower chamber. Incubated at 37^ºC for 12 hrs, then removed and wash twice by PBS. After incubation at 37^ºC for 12 hrs, the cells passed though the filters were washed twice by PBS and fixed with 4% of paraformaldehyde for 20 mins and subsequently stained with 1% crystal violet for 20 mins. The number of cells was counted under the microscope. At least three independent experiments were performed in each group.

Li H., Wang Y., Rong S.K., Li L., Chen T., Fan Y.Y., Wang Y.F., Yang C.R., Yang C., Cho W.C, & Yang J. (2020). Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer. International Journal of Biological Sciences, 16(5), 815-826.

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