Penicillin streptomycin

Human lung carcinoma cell line A549 and H1299; Human melanoma cell line UACC257 were cultured in RPMI (Corning, Cellgro) plus 10% Fetal Bovine Serum (FBS) (Gibco), 100 U/ml penicillin G and 100 μg/ml streptomycin (Corning, Cellgro). Human cervix adenocarcinoma cell line HeLa; Human prostate adenocarcinoma cell line PC-3; Normal Human fibroblast; Mouse embryonic fibroblast (MEF); Human Embryonic Kidney 293 cells; Human mammary gland adenocarcinoma cell line MCF7; Human bone osteosarcoma cell line U2OS were cultured in DMEM (Corning, Cellgro) plus 10% FBS, Penicillin-streptomycin antibiotics. Human mammary epithelial cell line MCF10A were cultured in DMEM/F-12 (Corning, Cellgro) plus 5% horse serum (Invitrogen), EGF (20ng/ml, Peprotech), Hydrocortisone (500ng/ml, Sigma), cholera toxin (100ng/ml, Sigma), Insulin (10ug/ml, Sigma), Penicillin-streptomycin antibiotics. Human glioblastoma cell line U87 were cultured in EMEM (Corning, Cellgro) plus 10% FBS, Penicillin-streptomycin antibiotics. Human colorectal carcinoma cell line HCT116 were cultured in McCoy's 5A (Corning, Cellgro) plus 10% FBS, Penicillin-streptomycin antibiotics. All cells were cultured in 37°C, 5% CO2 incubator.

MCF-7 (HER2 positive), MDAMB-231 (TNBC), BT-549 (TNBC), and SK-BR-3 (HER-2 positive) breast cancer cell lines were obtained from ATCC (Manassas, VA, USA). MCF-7, and MDAMB-231 were maintained in DMEM medium (Corning, NY, USA) supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA) and penicillin-streptomycin (Corning, NY, USA) at 5% CO₂/37 °C. BT-549 was maintained in RPMI-1640 medium supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA), 1% sodium pyruvate (Corning, NY, USA), 1% penicillin-streptomycin (Corning, NY, USA), 0.023 U/mL of Gibco™ Insulin, human recombinant zinc solution (Fisher Scientific, Hampton, NH, USA)). SK-BR-3 was maintained in Hyclone McCoy’s 5A Medium (GE Health care Life Sciences (Marlborough, MA, USA)) supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA) and 1% penicillin-streptomycin (Corning, NY, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), crystal violet dye, and 16% paraformaldehyde (PFA) solution were purchased from Fisher Scientific (Hampton, NH, USA). All molecular biology kits and supplies were purchased from other commercial vendors which are listed at appropriate places throughout the manuscript.

B16-F1 melanoma cells were obtained from American Type Culture Collection (CRL-6323) and maintained in Dulbecco's modified Eagle medium (DMEM; Corning) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals) and 1× penicillin-streptomycin (Corning). MNT-1 melanoma cells were a kind gift from Prof Michael S. Marks (Children’s Hospital of Philadelphia and University of Pennsylvania) and maintained in DMEM medium supplemented with 20% FBS, 10% AIM-V medium (Gibco), 1× nonessential amino (Corning), and 1× penicillin-streptomycin. SK-Mel-28, WM9, 501Mel, and 1205Lu melanoma cells were kind gifts from Prof Philip H. Howe (Medical University of South Carolina) and maintained in RPMI medium (Corning) supplemented with 10% FBS and 1x penicillin-streptomycin. All cells were cultured at 37 °C in a humid atmosphere with 5% CO₂.

Melanoma cancer cell line Mel Juso was cultured in DMEM that was supplemented with 1 g/L Glucose (Corning), 10% FBS (Biosera), and 100 U/ml penicillin/streptomycin (Corning). Melanoma cell lines: Mel Ju, A7, HMCB, WM239A, WM852, and A2058 were cultured in RPMI-1640 supplemented with 10% FBS and 100 U/ml penicillin/streptomycin (Corning). SK-MEL-3 and HT144 cells were cultured in McCoy’s 5A medium supplemented with 10% FBS (Biosera), 100 U/ml penicillin/streptomycin (Corning). Mel Juso, Mel Ju, Mel Ho, and SK-MEL-3 cells were kindly provided by Prof. Anja Bosserhoff (Friedrich-Alexander University Erlangen-Nuremberg, Germany). A7, HMCB, WM239A, WM852, HT144, and A2058 HT144 cells were kindly provided by Prof. Göran Jönsson (Lund University, Sweden). All cell lines were cultured at 37 °C in a humidified atmosphere containing 5% CO₂. Cells were trypsinized with 0.25% trypsin (Corning, Cat No. 25–053-CI). Cell Line authentication has been performed for all the cell lines presented in this study. Immortalized keratinocytes were cultured and transfected as described previously [24 (link)]. BCL3ANT was dissolved in DMSO at different concentrations and stored at -20 °C until use.