MiaPaCa2, MDA-MB231, Panc1, AsPC1, and HEK293T cell lines were obtained from American Type Culture Collection (ATCC) and mycoplasma-free. These cells were cultured in Dulbecco’s minimal essential medium (DMEM) from Nacalai (California, USA) supplemented with 10% v/v FBS and penicillin (100 U/mL)/streptomycin (100 μg/mL) from Hyclone (IL, USA). Antibodies for GAPDH (14C10, #2118), phospho-AKT Ser473 (#9271), phospho-ERK Thr202/Tyr204 (#9101), phospho-S6 Ser235/236 (#2211), YAP (#4912), phospho-YAP Ser127 (#4911), and phospho-LATS1 Ser909 (#9157) were from Cell Signaling Technology (MA, USA). Antibody for TAZ (#HPA007415) was from Sigma-Aldrich (MO, USA). Gemcitabine-HCl (#S1149) was obtained from Selleck Chemicals (TX, USA), while doxorubicin-HCl (#D-4000) from LC Laboratories (MA, USA). PD184352, Triciribine and Rapamycin were obtained from Sigma-Aldrich (MO, USA). The Cell viability was assayed by colorimetric based CellTiter 96® AQueous One Solution Cell Proliferation kit (#G3581, Promega), per manufacture’s protocol.
Triciribine
Manufactured by Merck Group
Sourced in United States
Triciribine is a laboratory compound that inhibits cellular enzymes involved in nucleic acid synthesis. It is used in research settings to study cellular processes and is not intended for medical use.
Automatically generated - may contain errors
Lab products found in correlation
Rapamycin, by Merck Group (4 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions)
Ly294002, by Merck Group (3 mentions)
Panc 1, by American Type Culture Collection (2 mentions)
Resveratrol, by Merck Group (2 mentions)
Wortmannin, by Merck Group (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Phospho erk thr202 tyr204, by Cell Signaling Technology (1 mentions)
Phospho s6 ser235 236, by Cell Signaling Technology (1 mentions)
Phospho yap ser127, by Cell Signaling Technology (1 mentions)
Phospho lats1 ser909, by Cell Signaling Technology (1 mentions)
Gemcitabine hcl, by Selleck Chemicals (1 mentions)
Pd184352, by Merck Group (1 mentions)
Doxorubicin hcl, by LC Laboratories (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
18 protocols using triciribine
1
Anticancer Drug Screening in Cell Lines
2
Chondrocyte Signaling Pathway Analysis
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Abcam Inc (Cambridge, MA, USA) was the source of the antibodies for PPARα, Collagen II, MMP13, ADAMTS5, and P62. Cell Signaling Technology Inc (Beverly, MA, USA) provided the antibodies for Akt, p-Akt (Ser473), ERK, p-ERK (Thr202/Tyr204), Beclin 1, and ERK inhibitor U0126. Sigma-Aldrich in China (Shanghai, China) supplied the antibody for Aggrecan, Akt inhibitor Triciribine (TCN), and autophagy inhibitor chloroquine diphosphate salt (CQ). Proteintech Group, Inc (Rosemont, IL, USA) provided the antibodies for LC3B and Bcl2. Recombinant human IL-1β was obtained from PeproTech China (Suzhou, China) and β-actin was obtained from Shanghai Abways Biotechnology (Shanghai, China).
3
Molecular Markers of Autophagy Regulation
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We obtained triciribine, rapamycin, AICAR, resveratrol, spermidine, and antibody against SQSTM1/p62 from Sigma-Aldrich (USA). We also purchased antibodies against CK2α, CK2β, β-actin, SIRT1, and siRNA for SIRT1 (sc-40986) from Santa Cruz Biotechnology (USA). We obtained antibodies against LC3, Atg5, Atg7, Atg5 (D5F5U), AMPK, phospho-AMPK (T172), FoxO3a, LKB1, and phospho-LKB1 (S428), from Cell Signaling Technology (USA).
4
Intracellular Signaling Pathway Analysis
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Dopamine, (−) quinpirole, isoproterenol, forskolin, 4-amino-5-(4-chlorophenyl)-7-(dimethyl ethyl) pyrazolo [3,4-d] pyrimidine (PP2), wortmannin, triciribine, agarose beads coated with monoclonal antibodies against FLAG epitope, rabbit anti-FLAG M2 antibodies (AB_439687), rabbit antibodies against green fluorescent protein (GFP)(AB_439690), and HA antibodies (AB_2610070) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Goat anti-mouse Alexa Fluor® 555 (AB_2535844), anti-rabbit Alexa Fluor® 555 (AB_2535849), anti-rabbit HRP-conjugated secondary antibodies (AB_10960844), and DAPI were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies to β-actin (AB_10950489), lamin b1 (AB_10896336), phospho-T308-Akt (AB_331170), phospho-S473-Akt (AB_331163), PDK1 (AB_2236832), phospho-S241-PDK1 (AB_2161134), phospho-Y416-Src (AB_331697), and arrestin2/3 (AB_10547883) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Antibodies to USP33 (AB_2213243), importin β1 (AB_2133993), phospho-ERK1/2 (AB_1125801), and ERK2 (AB_2141292) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti-mouse HRP-conjugated secondary antibodies (AB_10015289) were obtained from Jackson ImmunoResearch (West Grove, PA, USA).
5
Triciribine, Calcitriol, and Amphotericin B
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The Akt inhibitor triciribine, calcitriol and amphotericin B were purchased from Sigma-Aldrich Co. (St. Louis, MO, United States). A stock solution of calcitriol was prepared using DMSO as vehicle (<0.01% DMSO).
6
Nuclear Receptor Modulation of Viral Infection
7
Cell Culture and Drug Treatment
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U251 and SH-SY5Y cells (ATCC, USA) were cultured and maintained in Dulbecco’s Modified Eagle Media (DMEM) Ham’s F-12 (Sigma-Aldrich) supplemented with 10% heat inactivated fetal bovine serum (FBS) (Sigma-Aldrich) and 1% Penicillin/Streptomycin (Sigma-Aldrich). Cells were incubated at 37° C in a humidified incubator containing 5% CO2. The drugs Triciribine and Rapamycin were purchased from Sigma-Aldrich and were both reconstituted in dimethyl sulfoxide (DMSO), per manufacturer’s instructions.
8
Partial Liver Warm Ischemia-Reperfusion Injury Model
9
Evaluating Protein Interactions in Cell Signaling
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Antibodies against CK2α, p53, p21Cip1/WAF1, and β-actin were obtained from Santa Cruz Biotechnology (USA). An antibody against phospho-p53 serine 392 (S392) was purchased from Cell Signaling Technology (USA), and antibodies against HP1γ, SUV39h1, H3K9me3, histone H3, and MDM2 were from Abcam (UK). Both 4′,6-diamidino-2-phenylindole (DAPI) and rhodamine-conjugated goat anti-rabbit IgG were purchased from Invitrogen (USA). The anti-hemagglutinin (HA) antibody was obtained from Roche (Switzerland) and TrueBlot reagent was from Rockland (USA). Pifithrin-α, nutlin-3, cycloheximide, N-Acetyl-L-cysteine (NAC), wortmannin, triciribine, and rapamycin were obtained from Sigma Chemical (USA).
10
Sirtuin-Mediated Inflammatory Regulation
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Antibodies against SIRT1, CK2α, p53-p21Cip1/WAF1, RelA/p65, IκB, α-tubulin, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against acetylated RelA/p65 (ac-p65, K310), IKKα/β, phosphorylated IKKα/β (p-IKKα/β, S180/S181), IL-1β, IL-6, MMP3, and histone H3 were obtained from Abcam (Cambridge, UK). Antibodies against phosphorylated IκBα (p-IκBα, S32/S36) and phosphorylated AKT (p-AKT, S473) were obtained from Cell Signaling (Danvers, MA, USA). Resveratrol, triciribine, nicotine amide, and LPS were obtained from the Sigma Chemical Co. (St. Louis, MO, USA).
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