Anti iba1 rabbit pab

Manufactured by Wuhan Servicebio Technology

Sourced in China

Anti-Iba1 Rabbit pAb is a primary antibody that binds to the Iba1 protein. Iba1 is a calcium-binding protein that is specifically expressed in macrophages/microglia. This antibody can be used to detect and quantify Iba1 in various biological samples.

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Lab products found in correlation

Hrp conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (2 mentions) Cy3 conjugated goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions) Ds u3, by Nikon (1 mentions) Anti gfap rabbit pab, by Wuhan Servicebio Technology (1 mentions) Eclipse c1, by Nikon (1 mentions) He staining solution, by Wuhan Servicebio Technology (1 mentions) Fitc labeled goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions) Sds page gel rapid preparation kit, by Wuhan Servicebio Technology (1 mentions) Cy3 labeled goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions) Bca protein assay kit, by Solarbio (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)

3 protocols using anti iba1 rabbit pab

Immunohistochemical Evaluation of Immune Markers

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The paraffin sections were rehydrated with gradient ethanol solution. After antigen repair, the endogenous peroxidase activity was eliminated. The sections were blocked before incubation with the primary antibody (SLC1A5, abcom, ab237704; Anti -Iba1 Rabbit pAb, Servicebio, GB113502; Anti -CD86 Rabbit mAb, Servicebio, GB13585; Anti -CD163 Rabbit pAb, Servicebio, GB113152). SP Rabbit HRP Kit (DAB) (Cat.CW20355, CWBiotech) provides the protocol for the procedure. The results of IHC were scored according to the German Immunohistochemical Scores, and staining intensity at or above 4 points was defined as positive expression [47 (link)].

Han L., Zhou J., Li L., Wu X., Shi Y., Cui W., Zhang S., Hu Q., Wang J., Bai H., Liu H., Guo C., Cao H., Chao M., Hu Y., Mou Y., Jiao Y., Feng D., Wang L, & Qu Y. (2022). SLC1A5 enhances malignant phenotypes through modulating ferroptosis status and immune microenvironment in glioma. Cell Death & Disease, 13(12), 1071.

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Immunohistochemical Analysis of Hippocampal Neuroinflammation

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Brains were rapidly removed, frozen at −80 °C, and cut into 2-mm coronal slices in a rat brain matrix. After 10% formalin fixation, hippocampal tissue samples were processed into paraffin-embedded blocks and 5-μm-thick sections were cut for immunostaining. Hippocampal CA1 region sections were deparaffinized and treated with EDTA antigen repair buffer (pH 8.0). Antibodies used were anti-ionized calcium binding adaptor molecule 1 (IBA-1; anti-Iba1 rabbit pAb, Servicebio, HRP anti-rabbit IgG (HRP-conjugated goat anti-rabbit IgG, Servicebio), anti-glial fibrillary acidic protein (anti-GFAP; anti-GFAP rabbit pAb, Servicebio), and Cy3 anti-rabbit (Cy3-conjugated goat anti-rabbit IgG, Servicebio). Finally, the sections were sealed with the antifluorescence solution (G1401-5ML, Servicebio), and the immunofluorescence images were observed and recorded with fluorescence microscope (NIKON ECLIPSE C1, NIKON DS-U3).

Li P., Huang W., Yan Y.N., Cheng W., Liu S., Huang Y., Chen W., Chen Y.P., Gao Y., Lu W., Xu Y, & Meng X. (2021). Acupuncture Can Play an Antidepressant Role by Regulating the Intestinal Microbes and Neurotransmitters in a Rat Model of Depression. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 27, e929027-1-e929027-12.

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Detailed NLRP3 Inflammasome Activation Assay

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Chemicals are listed as follows: A804598 (HY-100483, MedChemExpress Co., Ltd. United States); IL-1Ra (HY-P7029A, MCE, United States); DMSO (D8370, Solarbio, China); Mouse NLRP3 ELISA kit (JYM0765Mo, Wuhan ColorfulGene biological technology Co., Ltd. China); Mouse IL-1β ELISA kit (JYM0531Mo, Wuhan ColorfulGene biological technology Co., Ltd. China); BCA Protein Assay Kit (PC0020, Solarbio, China); SDS-PAGE Gel Rapid Preparation Kit (G2037-50T, Servicebio, China); Recombinant Anti-beta Actin antibody (GB15003, Servicebio, China); NLRP3 Antibody (DF7438, Affinity Biologicals, Canada); Recombinant Anti-P2X7 antibody (ab259942, abcam, United Kingdom); HRP-conjugated goat anti-rabbit IgG (GB23204, Servicebio, China); Anti-Iba1 Rabbit pAb (GB11105, Servicebio, China); Cy3-labeled goat anti-rabbit IgG (GB21303, Servicebio, China); FITC-labeled goat anti-rabbit IgG (GB22303, Servicebio, China); Anti-BrdU Mouse mAb (GB12051, Servicebio, China); Anti-NeuN Rabbit pAb (GB11138, Servicebio, China); HE staining solution (G1005-100ML, Servicebio, China).

Yu Q., Liu M., Dai W., Xiong Y., Mu X., Xia M., Li Y., Ma S., Su Y., Wu J., Liu C., Xie Y., Zhao T., Lu A., Weng N., Zheng F, & Sun P. (2023). The NLRP3 inflammasome is involved in resident intruder paradigm-induced aggressive behaviors in mice. Frontiers in Pharmacology, 14, 974905.

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