H9 wa09 human embryonic stem cells

Manufactured by WiCell

H9 (WA09) human embryonic stem cells are a well-characterized and widely used line of pluripotent stem cells derived from a human blastocyst. These cells have the ability to differentiate into a variety of cell types, making them a valuable tool for research and potential therapeutic applications.

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Lab products found in correlation

Synthemax 2 sc substrate, by Corning (1 mentions)

2 protocols using h9 wa09 human embryonic stem cells

Biomedical Research Ethics Protocols

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All human cell lines and experimental protocols in the present study were used with approval and in accordance with policies of the following ethics committees: Women’s and Children’s Health Network Human Research Ethics Committee, South Australia, Australia [HREC/17/WCHN/70]; Salk Institute Institutional Review Board, La Jolla, USA. All subjects gave written informed consent to the derivation of iPSC and/or iN lines from skin biopsies, mutation screening and use of tissue for research. H9 (WA09) human embryonic stem cells were obtained from WiCell (Agreement No. 20-W0500).

van den Hurk M., Lau S., Marchetto M.C., Mertens J., Stern S., Corti O., Brice A., Winner B., Winkler J., Gage F.H, & Bardy C. (2022). Druggable transcriptomic pathways revealed in Parkinson’s patient-derived midbrain neurons. NPJ Parkinson's Disease, 8, 134.

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Feeder-free H9 hESC Culture

Cited 1 time

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H9 (WA-09) human embryonic stem cells were obtained from WiCell (Madison, WI) and, after initial feeder-based expansion on a layer of mitotically inactive human fibroblasts (D551), routinely cultured under feeder-free conditions on Synthemax II-SC substrate (Corning)-coated cell culture plates (as described previously [46 (link)]).

Thomas R., Menon V., Mani R, & Pruszak J. (2022). Glycan Epitope and Integrin Expression Dynamics Characterize Neural Crest Epithelial-to-Mesenchymal Transition (EMT) in Human Pluripotent Stem Cell Differentiation. Stem Cell Reviews and Reports, 18(8), 2952-2965.

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