Direct q 3 uv merck millipore purification system

Baicalin (≥95%, HPLC) was obtained from Sigma-Aldrich (Poznan, Poland). Excipients, such as chitosan with a degree of acetylation of 90% with a viscosity range of 500 mPas (marked as 90/500), was supplied from Heppe Medical Chitosan GmbH (Halle, Germany), (hydroxypropyl)methyl cellulose (HPMC) with an average Mn~90.000 (~15.000 cP), and magnesium stearate, were supplied by Sigma-Aldrich (Poznan, Poland). Microcrystalline cellulose (MCC) VIVAPUR 102 was supplied by JRS PHARMA (Rosenberg, Germany). Reagent for mucoadhesive tests: mucin from porcine stomach was obtained from Sigma-Aldrich (Poznan, Poland). HPLC grade acetonitrile and water were obtained from Merck. High-quality pure water and ultra-high-quality pure water were prepared using an Direct-Q 3 UV Merck Millipore purification system.

Baicalin (≥95%, HPLC), baicalein (≥98%, HPLC), and wogonin (phyproof^® Reference Substance) were obtained from Sigma-Aldrich (Poznan, Poland). Excipients, such as chitosans with a degree of acetylation of 70%, 80%, and 90% with a viscosity range of 500 mPas (marked as 70/500, 80/500, and 90/500, respectively) were supplied from Heppe Medical Chitosan GmbH (Halle, Germany). Reagents for activity assays: 2,2-Diphenyl-1-picrylhydrazyl (DPPH), sodium chloride, bovine serum, hexadecyltrimethylammonium bromide (CTAB), hyaluronic acid (HA), for dissolution studies: potassium chloride, sodium chloride, di-potassium hydrogen orthophosphate, magnesium chloride, calcium chloride, and xylitol, and mucoadhesive tests: mucin from porcine stomach were obtained from Sigma-Aldrich (Poznan, Poland). HPLC grade acetonitrile and water were obtained from Merck. High-quality pure water and ultra-high-quality pure water were prepared using an Direct-Q 3 UV Merck Millipore purification system.

Resveratrol (≥99%, HPLC), polydatin (≥95%, HPLC) were obtained from Sigma-Aldrich (Poznan, Poland). Excipients, such as (2-hydroxypropyl)-β-cyclodextrin (HPβCD), average Mw~1460, and magnesium stearate, were supplied from Sigma-Aldrich (Poznan, Poland), and polyvinylpyrrolidone (PVP) as Kollidon^® 30, from BASF Pharma (Burgbernheim, Germany). Reagents for the dissolution studies: potassium chloride, sodium chloride, di-potassium hydrogen orthophosphate, magnesium chloride, calcium chloride and xylitol, and mucoadhesive tests: mucin from porcine stomach, were obtained from Sigma-Aldrich (Poznan, Poland). HPLC grade acetonitrile and water were obtained from Merck. High-quality pure water and ultra-high-quality pure water were prepared using a Direct-Q 3 UV Merck Millipore purification system.

Chlorogenic acid (Phyproof^® Reference Substance) as well as quercetin (≥95%) were obtained from Sigma-Aldrich (Poznan, Poland). Sodium carbonate, sodium hydroxide, DMSO, formic acid, methanol, ammonium acetate, and copper (II) chloride were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland). HPLC grade acetonitrile and water, as well as Folin–Ciocalteu phenol reagent, were from Merck (Darmstadt, Germany). Chlorogenic acid and gallic acid were purchased from Sigma-Aldrich (Poznań, Poland). All other chemicals were from Sigma-Aldrich Chemical Co. (Taufkirchen, Germany). High-quality pure water and ultra-high-quality pure water were prepared using a Direct-Q 3 UV Merck Millipore purification system (Burlington, MA, USA).

Tedizolid (purity >98%) were supplied by T&W GROUP (Shanghai, China). Hydroxypropyl-β-cyclodextrin (HP-β-CD) (purity >98%) was obtained from Sigma-Aldrich (Poznan, Poland). UHPLC grade Acetonitrile UHPLC grade was supplied by Merck KGaA (Darmstadt, Germany) and formic acid (100%) by Avantor Performance Materials (Gliwice, Poland). Hydrochloric acid, potassium bromide, and potassium dihydrogen phosphate were obtained from Avantor Performance Materials (Gliwice, Poland). High-quality parent water was prepared by using a Direct-Q 3 UV Merck Millipore purification system (Molsheim, France).

Obtaining aqueous extracts from blue haskap berry leaves was preceded by grinding in a mill (2 times). Then, the raw material prepared this way was sieved through a 1 mm sieve to eliminate uncrushed leaf fragments. The extracts were prepared by weighing 1.0 g of the raw material into a flat bottom flask and adding 50 mL of distilled water obtained from the Direct-Q 3 UV Merck Millipore purification system (Merck, Darmstadt, Germany). The extraction was performed in a 30 °C ultrasonic bath (Bandelin Sonorex DT 1028 H; Bandelin, Germany, Berlin) for 60 min; the flask was kept off the light during the process. The ultrasonic bath was operated under predefined operating conditions at 35 kHz. The extracts were filtered through a filter paper to separate the remaining plant matrix from the extract. The extract prepared this way was divided into small portions and frozen at −20 °C to maintain the quantitative and qualitative composition throughout the research.

Baicalin, baicalein, and wogonin as phyproof^® reference substances, as well as sodium alginate—AlgNa, methyl cellulose—MC, poly(ethylene glycol)—PEG400 (M.W. 400), poloxamer 407—P407 (Kolliphor^® P407), and 2-hydroxypropyl cellulose—HPC were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Chitosan (Cs) 80:500 (degree of deacetylation: 77.6–82.5%; viscosity: 351–750 mPas in 1% acetic acid at ambient temperature) and 80:1000 (degree of deacetylation: 77.6–82.5%; viscosity: 751–1250 mPas in 1% acetic acid) were supplied from Heppe Medical Chitosan GmbH (Halle, Germany). High-quality pure water and ultra-high-quality pure water were prepared by using a Direct-Q 3 UV Merck Millipore purification system. Solvents used for the determination of total flavonoid content and HPLC method were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland). All other chemicals were from Sigma–Aldrich Chemical Co.