Tlrl pic

Manufactured by Merck Group

Tlrl-pic is a laboratory equipment product manufactured by Merck Group. It is designed for use in research and scientific applications. The core function of Tlrl-pic is to provide a controlled environment for various experimental procedures.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using tlrl pic

HIV Latency Reversal and Reactivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

To drive HIV emergence from latency and/or HIV expression increase, we used TNF-α (Invitrogen, Cat. #PHC3015), poly(I:C) (InvivoGen, #tlrl-pic), and METH (Sigma-Aldrich, Cat. #51-57-0) alone or in combination at the concentrations stated in the corresponding Figures and/or Figure Legends. To study the involvement of the σ receptors in METH-mediated HIV reactivation, BD1047 (Tocris, Cat. #0956), rimcazole (Tocris, Cat. #1497), and SM21 (Tocris, Cat. #0751) were used at the concentrations specified in the Figure Legends.

Alvarez-Carbonell D., Ye F., Ramanath N., Garcia-Mesa Y., Knapp P.E., Hauser K.F, & Karn J. (2019). Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathogens, 15(12), e1008249.

+ Open protocol

+ Expand

Exosome Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: mouse anti-CD9 (Santa Cruz Biotechnology, sc-59140), mouse anti-CD63 (BD Pharmingen, 556019), mouse anti-CD81 (Santa Cruz Biotechnology, sc-555675), mouse anti-β-actin (Millipore, MAB1501), mouse anti-MHC-2 (Cell Signaling Technology, 68258), mouse anti–NK-κB (Cell Signaling Technology, 8242), mouse anti–phosphor-NK-κB (Cell Signaling Technology, 3033), rabbit anti-ANXA1 (Thermo Fisher Scientific, 71-3400), rabbit anti-FPR2 (Novusbio, NLS1878), mouse anti-HT2-280 (Terrace, TB-27AHT2-280), and rabbit anti-SFTPC (Millipore, AB3786). Additionally, the following reagents were used: the Human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813), Phorbol 12-myristiate-12 acetate (PMA Sigma-Aldrich, P8139), poly (I:C) (InvivoGen, tlrl-pic), lipopolysaccharide LPS (Sigma‐Aldrich, L2630), dexamethasone (Fujifilm, 041-18861), recombinant ANXA1 (American Research products, 01-2062), and WRW4 (R&D Systems, 2262/1).

Fujita Y., Kadota T., Kaneko R., Hirano Y., Fujimoto S., Watanabe N., Kizawa R., Ohtsuka T., Kuwano K., Ochiya T, & Araya J. (2024). Mitigation of acute lung injury by human bronchial epithelial cell-derived extracellular vesicles via ANXA1-mediated FPR signaling. Communications Biology, 7, 514.

+ Open protocol

+ Expand

Murine Macrophage Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

pIC was from InvivoGen (tlrl-pic), formaldehyde and glycine were from Sigma-Aldrich, LPS was from Sigma-Aldrich (Escherichia coli 055:B5; L2880), and recombinant mouse IFN-γ was purchased from ImmunoTools (12343534). Trizma base, glycine, EDTA, β-glycerophosphate, PMSF, leupeptin, pepstatin A, aprotinin, SDS, and Triton X-100 were from Sigma-Aldrich. Sodium fluoride (NaF) was from Merck. CellTracker Green 5-chloromethylfluorescein diacetate was from Thermo Fisher Scientific (C2925). Polyornithine, Hoechst 33342, β-mercaptoethanol, and phalloidin-tetramethyl-rhodamine B isothiocyanate were from Sigma-Aldrich.

Buxadé M., Huerga Encabo H., Riera-Borrull M., Quintana-Gallardo L., López-Cotarelo P., Tellechea M., Martínez-Martínez S., Redondo J.M., Martín-Caballero J., Flores J.M., Bosch E., Rodríguez-Fernández J.L., Aramburu J, & López-Rodríguez C. (2018). Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. The Journal of Experimental Medicine, 215(11), 2901-2918.

+ Open protocol

+ Expand

Multiplex Assay for Cell Death

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reagents were as follows: recombinant human IFN-beta protein: R&D Systems, 8499-IF-010/CF; poly(dA:dT): InvivoGen, tlrl-patn; poly(I:C): InvivoGen, tlrl-pic; 2′,3′-dideoxycytidine: Sigma-Aldrich, D5782; deoxyribonucleic acid sodium salt from herring testes (HT-DNA): Sigma-Aldrich, D6898; MitoTracker: Invitrogen, M22426; IncuCyte Nuclight Rapid red dye: Sartorius, 4717; IncuCyte Cytotox red dye: Sartorius, 4632; IncuCyte Cytotox green dye: Sartorius, 4633; doxycycline: Sigma-Aldrich, D9891; staurosporine: Cell Signaling Technology, 9953; recombinant human TNF-alpha protein: R&D Systems, 210-TA; BV6: Selleckchem, S7597; z-VAD-FMK: Cell Signaling Technology, 60332; LPS: Cell Signaling Technology, 14011; nigericin: Cayman Chemical, 11437; Hoechst 33342: Thermo Fisher Scientific, 62249.

Nassour J., Aguiar L.G., Correia A., Schmidt T.T., Mainz L., Przetocka S., Haggblom C., Tadepalle N., Williams A., Shokhirev M.N., Akincilar S.C., Tergaonkar V., Shadel G.S, & Karlseder J. (2023). Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis. Nature, 614(7949), 767-773.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!