Dopamine hydrochloride da

BaTiO₃ particles, binders, plasticizers, and dispersants were obtained from Samsung Electromechanics (Seoul, Republic of Korea). Dopamine (DA) hydrochloride and hydrochloric acid (HCl 37%) were purchased from Sigma-Aldrich (Burlington, MA, USA).

The rat brain samples were thawed at room temperature, and 500 μL of perchloric acid (0.4 M) were added, mixed, and centrifuged at 15,000 rpm for 3 min. Supernatant (100 μL) was added to 200 μL mobile phase, and 20 μL of the mixture were injected into the chromatography. The peak area was recorded, and was quantified using the standard curve. The standard solution series were determined, and the peak area and concentration were calculated using least square linear regression. The chromatographic conditions were as follows: column, diamond-C18 (250 mm × 4.6 mm, 5 μm); mobile phase, 4.04 g anhydrous sodium acetate, 0.80 g heptane sodium sulfonate, and 0.188 g EDTA dissolved in 1,000 ml water, filtered using 0.25 μm filter membrane, pH adjusted to 4.5 with acetic acid, then to 3.8 with phosphoric acid, and 220 ml methanol added; flow rate, 1.0 ml/min; detector potential, 0.75 V; column temperature, 35°C; and injection volume: 20 μL. Dopamine (DA) hydrochloride (Sigma, United States), norepinephrine (NE) (Sigma, United States), and 5-hydroxytryptamine (5-HT) hydrochloride (Sigma, United States) were dissolved in methanol to prepare a standard solution for control. The levels of NE, DA, and 5-HT of NAc were determined.

All chemicals used were of analytical grade. Reagents were stored in hard dark glass bottles with glass stoppers to avoid leaching of fluorescent contaminants: acidified N-butanol (0.85 mL of concentrated HCl was added to 1 L of N-butanol), N-heptane, hydrochloric acid (0.1 N and 10 N), sodium hydroxide (5 N), acetic acid (0.2 N and 5 N), iodine (0.1 N; 1.27 g of iodine was dissolved in 100 mL of absolute ethanol), EDTA (0.12 M; 0.1-M EDTA was dissolved in 1-N sodium acetate and the pH was adjusted to 7.0 with 5-N sodium hydroxide), NaHCO₃ (0.033 M), and alkaline sulphite [25% (W/V), 0.5 g of anhydrous sodium sulphite was dissolved in 2 mL distilled water]. This solution was mixed well with 18 mL of 1 N NaOH just before use. O-phthalaldehyde (OPT; 4 mg and 10 mg) was dissolved in 10-N hydrochloric acid. Dopamine (DA) hydrochloride, norepinephrine (NE) hydrochloride, serotonin (5-HT), and hydrogen oxalate (obtained from Sigma-Aldrich, St. Louis, USA) were used in the experiments.

The modified Gel-DA (with a degree of substitution of dopamine of 13.6%) and DOHA (The TNBS method was 33.2% measured by oxidation degree of OHA) were supplied by the Biomaterials Research Group at Jinan University. Gelatin (type A, 300 bloom), HA (The M_n is 150 000– 300,000 Da), Dopamine (DA) hydrochloride, hexadecyl trimethyl ammonium bromide (CTAB), bis[3-(triethoxysilyl)propyl] tetrasulfide (BTES), Tetraethoxysilane (TEOS), and Iron (III) chloride hexahydrate (FeCl₃•6 H₂O) were purchased from Sigma-Aldrich. Dexamethasone sodium phosphate (Dex-p) was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Tianjin Damao Chemical Reagent Factory provides the other chemical reagents used in this research.

Graphene oxide (GO) powder was purchased from Graphenea (San Sebastian, Spain). Dopamine (DA) hydrochloride and Tris buffer were purchased from Sigma Aldrich (St. Louis, MO, USA). Graphitized mesoporous carbon (MC) and Gum Arabic (GA) powders were purchased from Sigma Aldrich (Madrid, Spain).

LDL was procured from Millipore (Burlington, MA, USA). Dopamine (DA) hydrochloride, primary antibody anti-β-lipoprotein, human serum albumin (HSA) and secondary antibody anti-chicken (IgG) were obtained from Sigma-Aldrich. Mono-(6-ethanediamine-6-deoxy)-β-Cyclodextrin (NH₂-β-CD) was acquired from Zhiyuan Biotechnology Co., Ltd. (Shandong, China). Polysulfone granules were provided by Zhejiang Qinyuan Water Treatment S.T. Co., Ltd. (Shanghai, China). Tris(hydroxymethyl)aminomethane was purchased from Amresco (Solon, OH, USA). Other chemicals were sourced from Sinopharm Chemical (Shanghai, China). Ultrapure water (18.2 MΩ cm) used in this study was prepared using an ELGA Classic UF system.