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2 protocols using sodium nitroprusside

1

Synthesis and Characterization of Bioactive Compounds

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Anisaldehyde, 3,5-dichloroaniline, and monohydrated hydrazine were purchased from Merck (Darmstadt, Germany), phenol, ascorbic acid, urease (from jack bean), DPPH (2,2-diphenyl-1-picrylhydrazyl), acetylthiocholine iodide (ATCI), acetylcholinesterase, and 5,5-ditiobis(2-nitrobenzoic) acid (DTNB) were from Sigma-Aldrich (Steinheim, Germany). Sodium hypochlorite, and sodium salicylate were from Daejung (Siheung City, Korea). Lithium chloride, sodium nitrite and sodium nitroprusside were from Riedel de Haën (Seelze, Germany). Urea, ethylenediaminetetraacetic acid and thiourea were from BDH Labs (Cambridge, England).
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2

Antioxidant Capacity Assessment Protocols

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Folin–Ciocalteu and gallic acid-1-hydrate (C6H2(OH)3COOH.H2O) were from PanReac (Castellar del Vallès, Barcelona); tannic acid, TPTZ 2,4,6-tri (2-pyridyl)-s-triazine, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid), potassium persulfate (K2S2O8), NADH (nicotine-adenine dinucleotide reduced), NBT (nitro blue tetrazolium) ≥97%, and PMS (phenazine methosulfate) were from Sigma-Aldrich (Buenos Aires, Argentina); 1-butanol, NED (1- naphthyl ethylenediamine dihydrochloride), and sulfanilic acid were from Merck (Darmstadt, Germany); procyanidin A2 was from Extrasynthese (Genay, França); potassium iodate was from VWR Analar Normapur; and sodium nitroprusside was from Riedel-de Haën (Charlotte, NC, USA). All reagents were pro analysis grade. All absorbance measurements were performed in a Perkin-Elmer Lambda 25 (Watertown, MA, USA). The reagents were weighed on an analytical balance (Sartorius ± 0.0001 g).
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