The human HCC cell lines, HCC-LM3 and SMMC-7721, as well as the normal liver cell line LO2, were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in RMi-1640 (Gibco, NY) or DMEM (Gibco, NY) medium supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (P/S; Gibco, USA). The cell cultures were maintained in a humidified incubator (Thermo Scientific, Waltham, MA, USA) at 37°C with 5% CO2.
Rmi 1640
Manufactured by Thermo Fisher Scientific
Sourced in United States
The RMI-1640 is a laboratory instrument designed for high-performance liquid chromatography (HPLC) analysis. It functions as a refractive index detector, providing sensitive and accurate measurements of changes in the refractive index of liquids flowing through the instrument.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Humidified incubator, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
5 fu f6627 10g, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Ges 1, by Procell (1 mentions)
Mkn 45, by Procell (1 mentions)
Bgc 823, by Procell (1 mentions)
Hgc 27, by Procell (1 mentions)
Sgc 7901, by Procell (1 mentions)
Kato 3, by Procell (1 mentions)
3 protocols using rmi 1640
1
Culturing Human Liver Cell Lines
2
Culturing Colorectal Cancer Cell Lines
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Human colorectal cancer cell lines HT-29, HCT-116, SW480, SW620, LoVo, SW48, DLD-1, Caco-1 and HCT-15 cells were maintained in RMI-1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% FBS, 200 mM Lglutamine, 1%PS, and placed in a wet incubator at 37°C and 5% CO2 (link). 5-FU (F6627-10G) was purchased from Sigma (Poole, UK), and soluble in dimethyl sulfoxide. Colorectal cancer cells were treated with 5-FU at 5 μg/mL.
3
Evaluating RP11-789C1.1 in ATR-CHK1 Signaling
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Human GC cell lines (AGS, MKN-45, BGC-823, KATOIII, HGC-27, and SGC-7901) and normal gastric epithelial cells (GES-1) were obtained from Procell Life Science & Technology Co., Ltd., Wuhan, China and Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China. All cell lines were verified by mycoplasma detection and short tandem repeat (STR) profiling. Cells were cultured in RMI-1640 supplemented with 10% fetal bovine serum (Gibco BRL, Grand Island, USA) and Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Grand Island, USA) at 37°C in 5% CO2 atmosphere. To evaluate the effects of RP11-789C1.1 in ATR-CHK1 signaling pathway, the cells were treated with concentrations of AZD6738 (1 µmol/L, S7693, Sellleck, Texas, USA) for 12 hours.
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