Facs canto 2 flow cytometer instrument

To explore the effect of AuNPs on the stemness characteristics of stem cells, the MSC expression profile of stem cells; CD90, CD44, CD105, and CD73 were tested before and after treatment with AuNPs by using flow cytometry. Finally, the samples were analyzed by using FACS DIVA software version 8, using BD FACS Canto II flow cytometer instrument (BD Biosciences, NJ, USA). Data interpretation was performed using Flowlogic software version 7.3 (Melbourne, Australia).

Flow cytometry analysis was performed to determine any disruption during the cell cycle of HCT-116 cells in the presence of Dis. HCT-116at density of 2 × 10⁶ cells were seeded and treated for 24 h, 48 h and 72 h in a 75 cm² flask. Negative control was treated with 0.02% DMSO. After fixation of the cells with 70% ethanol, cells were preserved in −20 °C overnight followed by washing and staining with 500 µl of PI/Rnase dye (BD Biosciences, San Jose, CA, USA) and incubated for 30 min. DNA content analysis was performed using BD FACSCanto II flow cytometer instrument and BD FACS Diva software (BD FACSCanto™II, San Jose, CA, USA). A total of 15,000 events per sample were recorded for analysis. ModFit LT version 3.0 software was used for analyzing the data.