Mouse gm csf and il 4

A peroxidase-labeled mouse anti-human IgE Fc antibody and peroxidase-labeled goat anti-mouse IgE, IgG1 and IgG2a Fc antibodies were obtained from SouthernBiotech, USA (9160-05, 1110-05, 1070-05 and 1155-05); tetramethylbenzidine (TMB) was purchased from Solarbio, China (R1200); aluminum hydroxide was obtained from Thermo Fisher, USA (77161); and LPS was purchased from Sigma, USA (L3012). ELISA kits for IL-4, IFN-γ and TNF-α were obtained from Ebioscience, USA (88-7044, 88–7314 and 88–7324); ELISA kits for IL-5 and IL-13 were purchased from 4 A Biotech, China (CME0003, CME0009); an IRF4 antibody was obtained from Cell Signaling, USA (4964); an antibody against GAPDH was procured from Proteintech, China (10494-1-AP); a TLR4 signaling inhibitor was purchased from Invivogen, USA (CLI-095); an anti-mouse TLR2 Ab was obtained from Biolegend, USA (121802); the PE-CD80, PE-CD83, FITC-MHCII and FITC-CD40 antibodies were obtained from Ebioscience, USA (12–0801, 12–0831, 11–5321 and 11–0402); and mouse GM-CSF and IL-4 were purchased from Sino Biological, China (51048-M07H, 51084-M08B). Anti-CD3 and anti-CD28 antibodies were obtained from Ebioscience, USA (16-0031-82, 16-0281-82).

PE-CD80, PE-CD83 and FITC-CD40 antibodies were purchased from Ebioscience, USA (12-0801, 12-0831 and 11-0402). Lipopolysaccharide (LPS) was purchased from Sigma, USA (L3012). Mouse GM-CSF and IL-4 were from Sino Biological, China (51048-M07H, 51084-M08B). Anti-CD3 and anti-CD28 antibodies were obtained from Ebioscience, USA (16-0031-82, 16-0281-82). Aluminum hydroxide was from Thermo Fisher, USA (77161). Peroxidase-labeled goat anti-mouse IgE, IgG1 and IgG2a Fc antibody were from Southernbiotech, USA (1110-05, 1070-05 and 1155-05). ELISA kits for IL-5 and IL-13 detection were from 4A Biotech, China (CME0003, CME0009). ELISA kits for IL-4 and IFN-γ were purchased from Ebioscience, USA (88-7044, 88-7314). Anti-mouse TLR2 antibody and Mouse IgG1, κ Isotype Ctrl were obtained from Biolegend, USA (121802, 400101). TLR4 signaling inhibitor was from Invivogen, USA (CLI-095). DNase I and collagenase D were from Sangon Biotech, China (B002004 and A004186). APC-CD45, FITC-NK-1.1, FITC-CD19 and PE-CD90 were obtained from Biolegend, USA (103111, 108705, 115505 and 205903). PerCP-IL-33R and FITC-Lineage antibodies were purchased from Ebioscience, USA (46-9333, 22-7770). PerCP-CD4, FITC-IL-4, PE-IFN-γ, PE-CD11c and PerCP-Siglec-F were purchased from Ebioscience, USA (46-0041, 11-7042, 12-7311, 12-0114 and 46-1702).