Cm120 tem

Manufactured by Philips

Sourced in Netherlands, United States

The Philips CM120 TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It provides a core function of magnifying and projecting an image of a specimen onto a viewing screen or a detector, allowing users to study the internal structure and composition of materials with exceptional detail.

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Lab products found in correlation

Ultrascan 4000sp ccd camera, by Ametek (3 mentions) Er 80 ccd, by Philips (3 mentions) Uc7 ultramicrotome, by Leica (2 mentions) Eponate 12 resin, by Ted Pella (2 mentions) Cx 41 phase contrast microscope, by Olympus (1 mentions) S 3700n sem, by Hitachi (1 mentions) Vitrobot, by Thermo Fisher Scientific (1 mentions) Ultrascan 1000, by Ametek (1 mentions) Tecnai t12 g2 tem, by Thermo Fisher Scientific (1 mentions) Multiscan 791, by Ametek (1 mentions) 2k x 2k ccd camera, by Ametek (1 mentions) Evo ma10, by Zeiss (1 mentions) Morada digital camera, by EMSIS (1 mentions) Item software, by EMSIS (1 mentions) Glutaraldehyde tem grade, by Merck Group (1 mentions) Orius ccd camera, by Ametek (1 mentions) Zetasizer nano, by Malvern Panalytical (1 mentions) Drx 250 avance spectrometer, by Bruker (1 mentions) Nano z, by Malvern Panalytical (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions)

43 protocols using cm120 tem

Transmission Electron Microscopy of Cells

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Samples were processed in the Electron Microscopy Core at Fudan Univeristy. Cell pellets were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer. The cells were washed with 0.1 M sodium cacodylate buffer and postfixed with 1% osmium tetroxide. The pellets were then dehydrated in graded ethanol series, infiltrated, and embedded in Spurr's resin. Samples were then polymerized for 48 h at 60°C, cut into 60-nm-thick sections on LKB-I microtome, positioned on 200 mesh grids, and stained with uranyl acetate and lead citrate. TEM was performed on PHILIPS CM120 TEM at an accelerating voltage of 120 Kv. Images were acquired with Gatan type UltraScan 4000SP CCD Camera connected to the TEM.

Lin S.J., Leng Z.G., Guo Y.H., Cai L., Cai Y., Li N., Shang H.B., Le W.D., Zhao W.G, & Wu Z.B. (2015). Suppression of mTOR pathway and induction of autophagy-dependent cell death by cabergoline. Oncotarget, 6(36), 39329-39341.

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Histological and Ultrastructural Analysis of Renal Cortex

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For histological assessments, small segments of renal cortex from the normal and experimental rats were fixed in 10% buffered formalin and were processed for paraffin sectioning. Sections of about 5 mm width were stained with hematoxylin-eosin (H&E) and Masson trichrome for assessment under light microscope (Olympus CX41 Phase Contrast Microscope, Japan). For electron microscopy (Philips CM120 TEM at 80 kV, Netherlands), renal cortex tubular tissues were fixed with 2.5% glutaraldehyde for 2 h and 1% osmium tetroxide for 2 h. The sample was dehydrated with a series of ethanol and acetone for 10 minutes, embedded in epoxy resin, and cut into ultrathin sections.

Peng L., Li J., Xu Y., Wang Y., Du H., Shao J, & Liu Z. (2016). The Protective Effect of Beraprost Sodium on Diabetic Nephropathy by Inhibiting Inflammation and p38 MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Rats. International Journal of Endocrinology, 2016, 1690474.

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Stereocilia and Cochlear Morphology Examination

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The morphology of the stereocilia of cochlear hair cells was examined using SEM. Samples for SEM were prepared following procedures described in our previous publications [46 (link)]. Briefly, the cochleae were fixed with 2.5 % glutaraldehyde. After dehydration, the samples were critical-point dried, mounted on aluminum stubs, sputter-coated with gold particles, and examined using a Hitachi S-3700N SEM (Japan). The cochlear SV morphology was observed on cochlear sections using light microscopy and TEM. For TEM, the cochleae after overnight fixation were decalcified, embedded in Epon resin, and sectioned on a Reichert Ultracut E ultramicrotome (Boeckeler Instruments, Tucson, NM). Ultrathin sections were mounted on formvar-coated slot grids, stained with lead citrate and uranyl acetate, and examined using a Philips CM 120 TEM. At least three independent samples were performed to confirm the results.

Chen L., Guo W., Ren L., Yang M., Zhao Y., Guo Z., Yi H., Li M., Hu Y., Long X., Sun B., Li J., Zhai S., Zhang T., Tian S., Meng Q., Yu N., Zhu D., Tang G., Tang Q., Ren L., Liu K., Zhang S., Che T., Yu Z., Wu N., Jing L., Zhang R., Cong T., Chen S., Zhao Y., Zhang Y., Bai X., Guo Y., Zhao L., Zhang F., Zhao H., Zhang L., Hou Z., Zhao J., Li J., Zhang L., Sun W., Zou X., Wang T., Ge L., Liu Z., Hu X., Wang J., Yang S, & Li N. (2016). A de novo silencer causes elimination of MITF-M expression and profound hearing loss in pigs. BMC Biology, 14, 52.

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Electron Microscopy of Heart Tissue

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The heart tissues were fixed in 2.5% glutaric dialdehyde for approximately 4 h and washed three times with 0.1 mL phosphate buffer. Next, the samples were embedded in Epon 812 and cut into 100‐nm thick slices using UC7 Ultramicrotome (Leica, Heerbrugg, Switzerland). The sections were then stained with uranyl acetate and lead citrate, and the images were captured by using an electron microscopy core facility with Philips (Amsterdam, the Netherlands) CM‐120 TEM (Amsterdam, the Netherlands).

Wang Q., Luo C., Lu G, & Chen Z. (2020). Effect of adenosine monophosphate‐activated protein kinase–p53–Krüppel‐like factor 2a pathway in hyperglycemia‐induced cardiac remodeling in adult zebrafish. Journal of Diabetes Investigation, 12(3), 320-333.

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Visualizing K18 and IAPP Protein Fibrils

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PFFs formed by K18 in the presence or absence of IAPP were visualized by negative stain TEM as described previously [24 ]. Tau PFFs and IAPP-tau PFFs were adsorbed to glow discharged 400 meshed carbon-coated copper grids for 2 min, quickly washed three times with Tris-HCl buffer (50 mM, pH 7.4), and floated upon two drops of 0.75% uranyl formate for 30 s. The grids were allowed to dry before imaging on a Phillips CM 120 TEM operating at 80 kV. The images were captured and digitized with an ER-80 CCD (8 megapixel) by advanced microscopy techniques.

Zhang G., Meng L., Wang Z., Peng Q., Chen G., Xiong J, & Zhang Z. (2022). Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer’s disease. Molecular Neurodegeneration, 17, 12.

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Cryo-EM Sample Preparation Protocol

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Cryo-TEM samples are prepared in a controlled environment vitrification system (CEVS) or using the commercial environmentally controlled automated Vitrobot (FEI, Netherlands), always at a controlled temperature (25 °C) and at saturation. A 6 µL drop of the suspension is placed on a 200-mesh TEM copper grid covered with a perforated carbon film. To remove excess solution and produce a thin liquid film the drop is blotted, manually in the CEVS and automatically in the Vitrobot. The blotted sample is then plunged into liquid ethane (−183 °C) to form a vitrified specimen and transferred to liquid nitrogen (−196 °C) for storage. Vitrified specimens are examined at temperatures below −175 °C using a Gatan 626 cryo holder either in a Tecnai T12 G2 TEM (FEI, Netherlands) or a Philips CM120 TEM operating at 120 kV. Images are recorded on a Gatan MultiScan 791 camera or Gatan UltraScan 1000 using the DigitalMicrograph software (Gatan, U.K.) in the low-dose imaging mode to minimize beam exposure and electron-beam radiation damage, as described.^{35 ,38}

Dong Y., Dorkin J.R., Wang W., Chang P.H., Webber M.J., Tang B.C., Yang J., Abutbul-Ionita I., Danino D., DeRosa F., Heartlein M., Langer R, & Anderson D.G. (2016). Poly(glycoamidoamine) Brushes Formulated Nanomaterials for Systemic siRNA and mRNA Delivery in Vivo. Nano letters, 16(2), 842-848.

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Protein Sample Preparation and Characterization

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Protein samples were expressed and purified by ViewPoint Therapeutics and shipped to UW-Madison as lyophilized samples. Samples were rehydrated in deuterated 1X PBS (from 10X PBS, Fisher) to a concentration of 3 mg/mL, immediately before use. All 2DIR data was measured at a concentration of 3 mg/mL. Room temperature samples were incubated at room temperature for 27 hours. Heated samples were heated for 2 hours at 43°C and then left at room temperature for 25 hours. TEM samples of crystallin proteins were prepared as previously described [10 (link)]. Briefly, equal parts protein (1 mg/mL) and 1% uranyl acetate were pipetted onto a TEM grid immediately before measurement. TEM in Fig 2 were collected with a Tecnai 12 TEM and in S3 Fig, with a Philips CM120 TEM. TIFF images were cropped in Adobe Illustrator to make figures.

Alperstein A.M., Molnar K.S., Dicke S.S., Farrell K.M., Makley L.N., Zanni M.T, & Andley U.P. (2021). Analysis of amyloid-like secondary structure in the Cryab-R120G knock-in mouse model of hereditary cataracts by two-dimensional infrared spectroscopy. PLoS ONE, 16(9), e0257098.

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Transmission Electron Microscopy Sample Preparation

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Transmission electron microscopy (TEM) samples were prepared as follows. One drop of the undiluted sample in either H₂O or chloroform (depending on the particle being analysed) was placed onto a carbon-coated copper grid and allowed to settle for 20 s; then, excess liquid was wicked away using filter paper. Imaging was performed with a CM120 TEM operating at 120 kV (Philips, Cambridge, MA, USA).

Mellor R.D., Schätzlein A.G, & Uchegbu I.F. (2021). Development of Bio-Functionalized, Raman Responsive, and Potentially Excretable Gold Nanoclusters. Nanomaterials, 11(9), 2181.

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Transmission Electron Microscopy of PFF

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10 μL of the PFF (5 μg/μl sonicated and unsonicated PFF in PBS) samples were pipetted onto a 200 mesh copper grids (EMS) with carbon-coated formvar film and incubated for 2 min. Excess liquid was removed by blotting. The grid was briefly placed on 10 μL of 2% uranyl acetate (w/v; Merck, Darmstadt, Germany), followed by blotting to remove excess liquid. This last step was repeated. Grids were allowed to dry before imaging on a Phillips CM 120 TEM operating at 80 kV. Images were captured and digitized with an US1000 CCD (2048 x 2048 pixel, 14 μm pixel, 100% fill factor) and 2k x 2k CCD camera (Gatan, Inc. USA) by advanced microscopy techniques.

Kim H., Park J., Kang H., Yun S.P., Lee Y.S., Lee Y.I, & Lee Y. (2020). Activation of the Akt1-CREB pathway promotes RNF146 expression to inhibit PARP1-mediated neuronal death. Science signaling, 13(663), eaax7119.

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Transmission Electron Microscopy of Extracellular Vesicles

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The 200-mesh copper grids coated with Formvar-carbon were glow-discharged, and 5-μL samples were directly loaded onto the grids for two minutes before absorbing the liquid excess. The samples were then negatively stained by floating for 30 seconds on a drop of 1.0% (w/v) sodium silicotungstate. After air drying, images were acquired using a Philips CM120 TEM operated at 80 kV. EV diameter was determined using ImageJ software [20 (link)]. The mean EV density per (sub)species was estimated from the number of vesicles observed in ten 5 μm² squares.

Gaurivaud P., Ganter S., Villard A., Manso-Silvan L., Chevret D., Boulé C., Monnet V, & Tardy F. (2018). Mycoplasmas are no exception to extracellular vesicles release: Revisiting old concepts. PLoS ONE, 13(11), e0208160.

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