εlisa

Albumin and serum glucose concentrations were measured by biochemical analyzer (Architect, ci 16200, Abbott).Hematocrit, hemoglobin and monocytes blood cells values were measured by hematological analyzer (Sysmex, xt-4000i, Roche).
The concentrations of beta2-microglobulin and insulin were measured by radioimmunoassays (Immunotech by Beckman, Czech Republic and BioSource Europe SA, Belgium respectively).
Insulin resistance was calculated using the homeostasis model assessment of insulin resistance (HOMA-IR) [12 (link)].
High sensitivity C-reactive protein (hsCRP) and serum tumor necrosis factor-a (TNF-a) concentrations were measured using enzyme linked immunoabsorbed assays (ΕLISA, Immundiagnostik AG., Germany and Ani Biotech Oy, Orgenium, Finland respectively) according to manufacturer’s specifications.
Normalized protein catabolic rate for dry body mass (nPCR) was calculated from the urea generation rate [13 (link)].Body mass index (BMI) was obtained from height and post-dialysis body weight.
Metabolic acidosis was defined by serum bicarbonate levels, which were measured using a blood gas analyzer (Roche, cobas b 121 system) taking care of the blood specimens [14 (link)].

Albumin, the ratio of low density lipoproteins (LDL) to high density lipoproteins (HDL) (LDL/HDL) and serum glucose concentrations were measured by biochemical analysis. Hematocrit, hemoglobin and monocytes blood cells values were also measured.
The concentrations of beta2-microglobulin, insulin and intact-parathormone (i-PTH) were measured by radioimmunoassays (Immunotech by Beckman, Prague, Czech Republic, BioSource Europe SA, Nivelles, Belgium and CIS bio international, Gif- sur-Yvette, France respectively).
Insulin resistance was calculated using the homeostasis model assessment of insulin resistance (HOMA-IR) [7 (link)].
High sensitivity C-reactive protein (hsCRP) and oxidized LDL (ox-LDL) serum concentrations were measured using enzyme linked immunoabsorbed assays (ΕLISA, Immundiagnostik AG., Bensheim, Germany) according to manufacturer’s specifications.
Normalized protein catabolic rate for dry body mass (nPCR) was calculated from the urea generation rate [8 (link)]. Body mass index (BMI) was obtained from height and post-dialysis body weight.

Albumin, calcium (Ca) corrected for the albumin levels, phosphate (P), high density lipoproteins (HDL) and low density lipoproteins (LDL) were measured by biochemical analysis, and hemoglobin values were also measured. The ratio of LDL/HDL was calculated.
High sensitivity C-reactive protein (hsCRP) and oxidized LDL (ox-LDL) serum concentrations were measured using enzyme linked immunoabsorbed assays (ΕLISA, Immundiagnostik AG, Germany and Immundiagnostik AG, Stubenwald-Allee, Bensheim, respectively) according to the manufacturer’s specifications.
The concentrations of intact-parathormone (i-PTH) were measured by radioimmunoassay (CIS bio international/France).
Metabolic acidosis was defined by serum bicarbonate concentrations less than 22.0 mmol/L, which were measured in a gas machine (Roche, combas b 121) by an electrode-based method taking care of the blood specimens [10 (link)].
The normalized protein catabolic rate for dry body mass (nPCR) was calculated from the urea generation rate [11 (link)]. The body mass index (BMI) was obtained from height and post-dialysis body weight.