1100 series hplc

Manufactured by Agilent Technologies

Sourced in United States, Germany, United Kingdom

The Agilent 1100 series HPLC is a high-performance liquid chromatography system designed for accurate and reliable analysis of a wide range of samples. It features advanced components and technologies to deliver consistent, high-quality results.

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1100 series (251 citations) 1100 series HPLC system (173 citations) Agilent 1100 series HPLC (135 citations) 1100 HPLC (111 citations) Agilent 1100 Series HPLC instrument (17 citations) Agilent 1100 Series Binary HPLC Pump (9 citations) 1100 Series HPLC Value System (9 citations) Agilent 1100 Series HPLC Value System (8 citations) Agilent 1100/1200 series HPLC system (7 citations) HPLC 1100 series binary pump (7 citations)

145 protocols using 1100 series hplc

HPLC Analysis of Free Fatty Acids

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Free fatty acids analysis was carried out with an Agilent Technologies 1100 series HPLC (Germany) fluorescence detector (FLD) system. Separation of fatty acid derivatives was carried out via a reversed-phase ODS C18 column (250 × 4.6 mm, 5 μm, Netherlands).

Hendawy O., Gomaa H.A., Hussein S., Alzarea S.I., Qasim S., Abdel Rahman F.E., Ali A.T, & Ahmed S.R. (2021). Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease. Saudi Pharmaceutical Journal : SPJ, 29(11), 1303-1313.

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HPLC Analysis of Carbohydrates and Organic Acids

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ODs were measured at 600 nm in a Spectronic 601 spectrophotometer (Milton Roy, Ivyland, PA, USA). Samples were then centrifuged and filtered through a 0.2-μm syringe filter before placing them in high pressure liquid chromatography (HPLC) vials. Samples were analyzed for carbohydrates and organic acids (succinic, formic, acetic, and lactic acid) via HPLC using the Shodex SP0810 carbohydrate column and the Biorad Aminex HPX-87H organic acids column.
Furfural, HMF, furfuryl alcohol, and HMF-alcohol were separated and quantified on an Agilent 1100 series HPLC equipped with a diode array detector (DAD). The wavelengths monitored were 250 nm to detect furfural and 225 nm for furfuryl alcohol, HMF and HMFA. Samples and standards were analyzed using an Agilent Zorbax SB-C18 5 um 4.6 × 250 mm with a guard column. A mobile phase of acetate buffer (12.5 mM, pH = 4.5) and acetonitrile (4:1) was run isocratic at 1.0 mL/min for 10 min. Concentrations for the standards in acetate buffer were <1.0 mg/mL and standard curves were generated and used for quantitation. Samples were prepared by diluting 0.4 mL of culture supernatant with 0.4 mL of acetate buffer (25 mM, pH = 4.5) and 0.2 mL of acetonitrile. The flowrate was 1 mL/min, column compartment temperature of 25 °C, and injection volume was 10 µL.

Salvachúa D., Mohagheghi A., Smith H., Bradfield M.F., Nicol W., Black B.A., Biddy M.J., Dowe N, & Beckham G.T. (2016). Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation. Biotechnology for Biofuels, 9, 28.

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HPLC Characterization of BPO Compounds

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In order to confirm the content of BPO in the real sample, HPLC was used as reference method. Here, briefly the operational conditions of HPLC (Agilent 1100 series HPLC, Germany) adapted from Saiz et al [17 (link)] were as follows: isocratic separation on a ACE® C-18-AR column (5 μm, 250 mm × 4.6 mm i.d.; Advanced Chromatography Technologies Ltd., United Kingdom); methanol/water (80:20) mobile phase at 1.0 mL/min; 20 μL of sample loop. The eluted compound was detected at 227 nm.

Ponhong K., Supharoek S.A., Siriangkhawut W, & Grudpan K. (2015). A rapid and sensitive spectrophotometric method for the determination of benzoyl peroxide in wheat flour samples. Journal of Food and Drug Analysis, 23(4), 652-659.

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Enzymatic Activity Assay with Cinnamic Acid

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Assays containing 10 mm cinnamic acid, 50 mm KCl, 50 mm NaP_i, pH 6, were incubated for 2 h at 25 °C with 50 μm enzyme. 100 μl of sample was added to 900 μl of 50% (v/v) H₂O/acetonitrile with 0.1% TFA. Sample analysis was performed using an Agilent 1100 series HPLC equipped with a UV detector. The stationary phase was a Kinetex 5-μm C18 column, 250 × 4.6 mm. The mobile phase was acetonitrile/H₂O (50:50), with 0.1% TFA. Detection was performed at 254 nm.

Bailey S.S., Payne K.A., Fisher K., Marshall S.A., Cliff M.J., Spiess R., Parker D.A., Rigby S.E, & Leys D. (2017). The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis. The Journal of Biological Chemistry, 293(7), 2272-2287.

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Molecular Weight of Natural Rubber

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Gel permeation chromatography (GPC) was used to determine the natural rubber molecular weight. For tissue cultured plants, cyclohexane extractables collected from ASE were re-suspended in approximately 3 mL of tetrahydrofuran (THF; Fisher Sci., United States) with gentle, overnight shaking (Multi-Purpose Rotator, Thermo Sci., United States). For greenhouse plants, washed rubber particles were solubilized directly in THF. Solutions were syringe-filtered through a 1.6 μm glass microfiber Whatman grade GF/A filter (GE Healthcare Life Sciences, United States), then 50 μL injected onto a Hewlett Packard 1100 series HPLC and size exclusion separated (THF continuous phase, 1.0 mL/min) by two Agilent PL gel 10 μm Mixed-B columns in series at 35°C. The first peak elutes at ∼12 mins by refractive index (RID; Agilent 1260 Infinity, dn/dc = 0.129) and light scattering (DAWN Heleos-II, Wyatt Technology, Santa Barbara, CA, United States) detectors and represents the high molecular weight natural rubber. Measurements were repeated at least three times with three technical replicates performed for each genotype.

Placido D.F., Dong N., Dong C., Cruz V.M., Dierig D.A., Cahoon R.E., Kang B.G., Huynh T., Whalen M., Ponciano G, & McMahan C. (2019). Downregulation of a CYP74 Rubber Particle Protein Increases Natural Rubber Production in Parthenium argentatum. Frontiers in Plant Science, 10, 760.

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TMT-11 Labeling and High pH Fractionation

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Peptides were labeled with TMT 11-plex reagents according to the instructions provided by the manufacturer. The reference sample was placed in channel 126 of the TMT 11-plex experiment in order to reduce the effect of isotopic cross-contamination, as a recent study has recommended. [60 (link)]. The labeled peptides were mixed together and then purified and concentrated on a C-18 Sep-Pak (Waters Chromatography Europe, Etten-Leur, The Netherlands). TMT-11 labelled peptides were fractionated by high pH RP-HPLC using a Phenomenex Aeris Widepore XB-C8 (3.6 μm, 2.1 × 100 mm) column on an 1100 Series HPLC (Agilent Technologies). Ninety-eight fractions were collected at 1 min intervals and further concatenated to 24 or 25 fractions. This procedure was repeated for the six batches of TMT 11-plex analyzed.

Betancourt L.H., Szasz A.M., Kuras M., Rodriguez Murillo J., Sugihara Y., Pla I., Horvath Z., Pawłowski K., Rezeli M., Miharada K., Gil J., Eriksson J., Appelqvist R., Miliotis T., Baldetorp B., Ingvar C., Olsson H., Lundgren L., Horvatovich P., Welinder C., Wieslander E., Kwon H.J., Malm J., Nemeth I.B., Jönsson G., Fenyö D., Sanchez A, & Marko-Varga G. (2019). The Hidden Story of Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma—Association with Clinical Outcome and Tumor Phenotypes. Cancers, 11(12), 1981.

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HPLC Analysis of Organic Compounds

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Sample analysis was performed using an Agilent 1100 Series HPLC equipped with a UV detector. The stationary phase was a Kinetex 5μ C18 100A column, 250 × 4.6 mm. The mobile phase was acetonitrile/water (50/50) with 0.1 % TFA at a flow rate of 1 mL/min, and detection was performed at a wavelength of 264 nm.

Payne K.A., Quezada C.P., Fisher K., Dunstan M.S., Collins F.A., Sjuts H., Levy C., Hay S., Rigby S.E, & Leys D. (2014). Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation. Nature, 517(7535), 513-516.

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CtsK-mediated α-synuclein fibrillation

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In glass vials, soluble (15 μM) or fibrillar α-syn (15 or 30 μM) was incubated with CtsK (5–150 nM) in reaction buffer (50 mM NaOAc, 20 mM NaCl, 5 mM DTT, pH 5) in a total volume of 250 μL. Samples were agitated at 600 rpm at 37 °C in a Mini-Micro 980140 shaker. Reactions (40 μL) were taken at 15 min, 30 min, 1 h, 2 h, 4 h, 16 h and 20 h and terminated with 2M GuHCl. Samples (30 μL) were separated using a reverse-phase C18 column (Zorbax 300SB-C18, 2.1 x 50 mm, 3.5 μm, Agilent Technologies) on either a 1100 series HPLC (Agilent Technologies) coupled to an Agilent G1946D mass selective detector (MSD) equipped with an electrospray ionization (ESI) interface or Agilent 6224 accurate TOF-LC/MS (Agilent technology, Delaware). Mass spectra were obtained using positive-ion mode. Data were analyzed using LC/MSD ChemStation software (Rev. B.04.03, Agilent Technologies).

McGlinchey R.P., Lacy S.M., Walker RL I.I.I, & Lee J.C. (2020). Cathepsin K Is a Potent Disaggregase of α-Synuclein Fibrils. Biochemical and biophysical research communications, 529(4), 1106-1111.

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Spectroscopic Analysis and Chiral HPLC Characterization

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¹H NMR and ¹³C NMR were recorded on a Bruker-400
MHz spectrometer (¹H NMR: 400 MHz; ¹³C NMR:
100 MHz) using TMS as an internal
reference. The chemical shifts (δ) and coupling constants (J) were expressed in ppm and Hz, respectively. UV–Vis
spectrophotometry was carried out on a Shimadzu UV-3000. HPLC analysis
was carried out on an Agilent 1100 series HPLC with a multiple wavelength
detector. Chiralpak AS-H, AD-H, and OD-H columns were purchased from
Daicel Chemical Industries, Ltd. Optical rotations were measured on
a PerkinElmer polarimeter (model 343). HRMS (ESI) was recorded on
Waters Q-TOF Premier and Thermo Scientific LTQ Orbitrap XL Hybrid
Ion Trap-Orbitrap mass spectrometers. Commercially available compounds
were used without further purification. Solvents were purified according
to the standard procedures, unless otherwise noted. Commercial pyrrole
should be distilled for the use of the reactions. Ligands^{9d ,10b} and various 2-enoyl-pyridine N-oxides 2(11 (link)) were prepared according to literature
procedures.

Sun J., Gui Y., Huang Y., Li J., Zha Z., Yang Y, & Wang Z. (2020). Lewis Acid-Catalyzed Enantioselective Friedel–Crafts Alkylation of Pyrrole in Water. ACS Omega, 5(21), 11962-11970.

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Comprehensive Chemical Characterization

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Samples were analyzed by HPLC coupled with mass spectrometry (HPLC-MS): the electrospray ionization mass spectrometry technique of the ESI-IT ion trap type (ElectroSpray Ionization-Ion Trap) on an Esquire HCT device (high capacity trap, Bruker Daltonics) was coupled with reversed-phase chromatographic analysis (Agilent 1100 series HPLC), with an Agilent Eclipse XDB C18 80A column, 5 μm, 4.6 mm × 150 mm. The gradient used was 5 to 100% B in 60 min at 1 mL/min, with a wash phase at 80% B for 10 min and a column equilibration phase at 95% A for 10 min. The data were analyzed with DataAnalysis software (Bruker Daltonics). The indicated retention times were given in minutes. Samples were also analyzed by mass spectrometry MALDI-TOF (Matrix-assisted laser desorption ionization Time of flight), using a 4800 MALDI TOF-TOF Analyzer (Applied Biosystems), in reflectron mode. The sample was co-crystallized on a plate with a solution of α-cyano-4-hydroxy-cinnamic acid (HCCA), solution prepared at 10 mg/mL in 50/50 CH₃CN/H₂0, 0.1% TFA. The reported m/z values correspond to the monoisotopic peak.

Van Baelen A.C., Iturrioz X., Chaigneau M., Kessler P., Llorens-Cortes C., Servent D., Gilles N, & Robin P. (2023). Characterization of the First Animal Toxin Acting as an Antagonist on AT1 Receptor. International Journal of Molecular Sciences, 24(3), 2330.

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