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The FVD520 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of the FVD520 is to provide sample processing and preparation capabilities to researchers and scientists.

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Lab products found in correlation

Apc anti mouse f4 80 antibody, by BioLegend (1 mentions) Anti apc microbeads, by Miltenyi Biotec (1 mentions) Celltracker red cmptx dye, by Thermo Fisher Scientific (1 mentions) Macs ls column, by Miltenyi Biotec (1 mentions) Met 5a cells, by American Type Culture Collection (1 mentions)

2 protocols using fvd520

1

Zymosan-Induced Peritoneal Macrophage Isolation

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In addition, we observed the above phenomena using confocal microscopy (TiEA1R, NIKON INSTECH Co. Ltd., Tokyo Japan) under the conditions of with or without AIM coating. Debris was stained with FVD520 (eBioscience). The peritoneal cells derived from the zymosan model mice were stained with APC anti-mouse F4/80 antibody (BioLegend, San Diego, CA) and incubated with Anti-APC microbeads (Miltenyi Biotec). F4/80 positive cells were separated using a MACS LS column (Miltenyi Biotec) stained with CellTracker™ Red CMPTX Dye (10 µM) (Thermo Fisher Scientific).
Tomita T., Arai S., Kitada K., Mizuno M., Suzuki Y., Sakata F., Nakano D., Hiramoto E., Takei Y., Maruyama S., Nishiyama A., Matsuo S., Miyazaki T, & Ito Y. (2017). Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice. Scientific Reports, 7, 6450.
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2

Coating Cell Debris with Apoptosis Inhibitor

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Human mesothelial cell-line (Met-5A) cells, which were purchased from American Type Culture Collection (Manassas, VA), were cultured as described previously3 (link), 59 (link). Met-5A cells were heat-killed by incubation at 65 °C for 20 min in PBS, and labeled with FVD520, −660 or −780 (eBioscience) for 30 min at 4 °C. For coating of the surface of the cell debris with AIM, labeled dead cell debris was divided into two groups, and was incubated with serum-free culture medium with or without rAIM at a concentration of 50 μg/ml at 37 °C for 1 h, as described previously29 (link).
Tomita T., Arai S., Kitada K., Mizuno M., Suzuki Y., Sakata F., Nakano D., Hiramoto E., Takei Y., Maruyama S., Nishiyama A., Matsuo S., Miyazaki T, & Ito Y. (2017). Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice. Scientific Reports, 7, 6450.
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