Dylight 550 goat anti gst

Manufactured by Cayman Chemical

DyLight 550 goat anti-GST is a secondary antibody that binds to glutathione S-transferase (GST) proteins. It is conjugated with the DyLight 550 fluorescent dye, which has an excitation/emission wavelength of 562/576 nm. This product can be used in various immunoassay and detection applications.

Automatically generated - may contain errors

Lab products found in correlation

Protoarray v5, by Thermo Fisher Scientific (2 mentions) Pa055, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 goat anti human igg antibody, by Thermo Fisher Scientific (2 mentions) Innoscan 1100 al, by Innopsys (1 mentions)

2 protocols using dylight 550 goat anti gst

Plasma Autoantibody Reactivity Profiling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma autoantibody reactivity was studied using full-length human protein arrays (ProtoArray v5.1, PAH05251020, Thermo Fisher) [40 (link), 41 ]. The patient and two healthy blood donors were investigated in the same experiment. Protein arrays were probed with plasma at a dilution of 1:2000, and otherwise following the manufacturer’s protocol. Protein arrays were first incubated with blocking buffer (PA055, Life Technologies) for 1 h, followed by 90-min incubation with plasma, and lastly, a 90-min incubation with detection antibodies: Alexa Fluor 647 goat anti-human IgG antibody (A21445, Thermo Fisher) at 1:2000 dilution and Dylight 550 goat anti-GST (#DY550011-13–001, Cayman Chemicals) at 1:10,000 dilution. The Innopsys InnoScan 1100 AL 3-channel ultra-high resolution microarray scanner was used for detection.

Abolhassani H., Landegren N., Bastard P., Materna M., Modaresi M., Du L., Aranda-Guillén M., Sardh F., Zuo F., Zhang P., Marcotte H., Marr N., Khan T., Ata M., Al-Ali F., Pescarmona R., Belot A., Béziat V., Zhang Q., Casanova J.L., Kämpe O., Zhang S.Y., Hammarström L, & Pan-Hammarström Q. (2022). Inherited IFNAR1 Deficiency in a Child with Both Critical COVID-19 Pneumonia and Multisystem Inflammatory Syndrome. Journal of Clinical Immunology, 42(3), 471-483.

+ Open protocol

+ Expand

Autoantibody Profiling of Pediatric COVID-19

Check if the same lab product or an alternative is used in the 5 most similar protocols

We performed autoantibody profiling on samples from healthy children (n = 11), children with CoV-2+ (n = 5), children with MIS-C (n = 12) and children with Kawasaki (n = 28) in two batches. Serum autoantibody reactivity was studied using full-length human protein arrays (ProtoArray v5.1, PAH05251020, ThermoFisher) (Zhu et al., 2001 (link)). Protein arrays were probed with serum at a dilution of 1:2000, and otherwise followed the protocol provided by the manufacturer for immune response biomarker profiling. Protein arrays were first incubated with blocking buffer (PA055, Life Technologies) for 1 hour, followed by 90 min incubation with serum at 1:2000 dilution, and 90 min incubation with detection antibodies: Alexa Fluor 647 goat anti-human IgG antibody (A21445, ThermoFisher) at 1:2000 dilution and Dylight 550 goat anti-GST (#DY550011-13-001, Cayman Chemicals) at 1:10,000 dilution. The LuxScan HT24 (BioCapital) microarray scanner was used.

Consiglio C.R., Cotugno N., Sardh F., Pou C., Amodio D., Rodriguez L., Tan Z., Zicari S., Ruggiero A., Pascucci G.R., Santilli V., Campbell T., Bryceson Y., Eriksson D., Wang J., Marchesi A., Lakshmikanth T., Campana A., Villani A., Rossi P., Landegren N., Palma P, & Brodin P. (2020). The Immunology of Multisystem Inflammatory Syndrome in Children with COVID-19. Cell, 183(4), 968-981.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!