Human cd14 quantikine kit

Levels of REG3α, TFF3, I-FABP (intestinal fatty acid binding protein), and IL-22 in plasma samples were quantified using the Human REG3α DuoSet ELISA Kit, Human TFF3 DuoSet ELISA kit, Human I-FABP DuoSet ELISA kit, and Human IL-22 Quantikine ELISA kit (R&D Systems, Minneapolis, MN), respectively, according to the manufacturer’s instructions. Plasma levels of sCD14, sCD163, LPS-binding protein (LBP), and IL-6 were quantified using the Human CD14 Quantikine Kit, the Human CD163 Quantikine Kit, the Human LBP DuoSet ELISA Kit, and the IL-6 High Sensitivity Quantikine ELISA kit (R&D Systems, Minneapolis, MN), respectively, as previously described (Li et al., 2017 (link), Li et al., 2019 (link), Xia et al., 2020 (link)). Plasma LPS levels were determined using the PYROGENT-5000 Kinetic Turbidimetric Limulus Amebocyte Lysate Assay (Lonza, Walkersville, MD) as previously described (Li et al., 2019 (link)). Plasma concentrations of 45 cytokines/chemokines/growth factors were measured using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex Panel 1 (ThermoFisher Scientific, Waltham, MA) as previously described (Li et al., 2019 (link)).

Levels of sCD146, sICAM-1, and sVCAM-1 in plasma samples were quantified using the Human CD146 DuoSet ELISA Kit, the Human ICAM-1 DuoSet ELISA Kit, and the Human VCAM-1 DuoSet ELISA Kit (all from R&D Systems, Minneapolis, MN), respectively. LPS amounts were determined using the LAL assay as previously descried (Li et al., 2019 ). Plasma levels of soluble CD163, CD14, and LBP were quantified using the Human CD163 Quantikine Kit, the Human CD14 Quantikine Kit, and the Human LBP DuoSet ELISA Kit (all from R&D Systems), respectively. Plasma concentration of VEGF-A and 44 other cytokines/chemokines/growth factors were measured by multiplex immunoassays as previously descried (Li et al., 2019 ).