Anti snap 25pthr138

Anti-SNAP-25, anti-actin, anti-Flag, anti-GST, anti-syntaxin, peroxidase conjugated anti-rabbit IgG and anti-chicken IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-SNAP-25^pThr138 (Abgent, San Diego, CA, USA), anti-MYPT1^1-296 [20 (link)], anti-PP1cδ (Millipore, Billerica, MA, USA), anti-GAPDH (Santa Cruz, CA, USA), anti-CPI17^pThr38 [22 (link)], horseradish peroxidase-linked anti-mouse IgG (Cell Signalling, Danvers, MA, USA), Clean-Blot IP Detection Reagent (Thermo Scientific, Waltham, MA, USA) and Texas Red-X phalloidin (Life Technologies, Carlsbad, CA, USA) were purchased as indicated. Alexa Fluor 488-conjugated anti-rabbit IgG, Alexa Fluor 546-conjugated anti-mouse IgG, Alexa Fluor 546-conjugated anti-goat IgG and To-Pro-3 iodide were obtained from Molecular Probes (Eugene, OR, USA).

Materials were obtained from the indicated sources. Anti-SNAP-25^pThr138 (Abgent, San Diego, CA, USA), HeLa (cervical carcinoma) cells and tsa201 cells (European Collection of Cell Cultures); Anti-SNAP-25, anti-FLAG resin, FLAG peptide, Minimum essential medium (MEM), okadaic acid (OA); Trypsin/EDTA, l-glutamine, foetal bovine serum, FM 2–10 styryl dye and SIGMA FAST Protease Inhibitor Cocktail Tablets, EDTA-Free (Sigma-Aldrich, St. Louis, Missouri, USA); [³²P]ATP[γP] (Hungarian Isotope Institute, Budapest, Hungary), CM5 sensor chip, N-ethyl-N’-dimethylaminopropyl-carbodiimide, N-hydroxysuccinimide, Amine Coupling Kit (Biacore AB, Uppsala, Sweden). All other chemicals used were purchased commercially in the highest purity available.