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Anti ybx1 antibody

Manufactured by Proteintech
Sourced in United States

The Anti-YBX1 antibody is a laboratory tool used to identify and detect the presence of the YBX1 protein in biological samples. YBX1 is a DNA/RNA-binding protein involved in various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of YBX1 in different cell types and tissues.

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2 protocols using anti ybx1 antibody

1

ChIP Assay for Protein-DNA Interactions

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The ChIP assay was performed using the BeyoChIP™ ChIP Assay Kit (Beyotime, Shanghai, China) according to the manufacturer’s instructions. Briefly, 1 × 107 CRC cells were prepared and then cross-linked with 1% formaldehyde for 15 min at room temperature. The cross-linking process was quenched by adding 0.125 M glycine. Chromatin was isolated with the lysis buffer provided in the kit. Afterwards, sonication was performed to shear the DNA to 200–1000 bp. Immunoprecipitation of cross-linked protein/DNA was performed by incubating anti-YBX1 antibody (Proteintech, IL, USA) or normal rabbit IgG (Proteintech, IL, USA) with sheared chromatin overnight at 4 °C followed by incubation with Protein A/G Magnetic Beads for 2 h. Subsequently, cross-links of protein/DNA were released with 5 M NaCl, and the DNA was purified using a PCR Clean Up Kit (Beyotime, Shanghai, China). Finally, the purified DNA was subjected to qRT–PCR using the P1-P9 primer sequences listed in Additional file 1: Table S4.
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2

Protein Extraction and Western Blotting

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Cells were lysed with NETN buffer on ice for 20 min and then centrifuged at 12,000 rpm for 20 min to extract proteins. Then, the proteins were separated by SDS-PAGE. The following antibodies were used: anti-CDKL1 antibody (Abcam, ab136129), anti-YBX1 antibody (Proteintech, 20339-1-AP), anti-GAPDH antibody (Proteintech, 60,004-I-Ig), anti-Flag-tag antibody (ABclonal, AE005), anti-Myc-tag antibody (ABclonal, AE010), anti-H3 antibody (Proteintech, 17168-1-AP), anti-α-tubulin antibody (Proteintech, 11224-1-AP), and anti-PD-L1 antibody (Proteintech, 17952-1-AP).
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