Phenotypes were scored in live WT, pierce1, pierce2 and the double knockout mutant embryos using a stereomicroscope. Phenotypes were analyzed in embryos at different stages. Otolith counts were performed at 20–22 hpf. Curved body axis and hydrocephaly were determined at 48 and 72 hpf. Kidney cysts and edema were scored at 4–5 days post fertilization (dpf). For left–right asymmetry, immunofluorescence microscopy was carried out at 36 hpf with anti-A4.1025 antibody (Developmental Studies Hybridoma Bank) to visualize heart jogging. Heart jogging was classified as left, right, or bilateral. To evaluate significance, we used the Fisher’s Exact Test (2 × 2 matrix, two-tailed).
Anti a4.1025 antibody
Manufactured by Developmental Studies Hybridoma Bank
The Anti-A4.1025 antibody is a laboratory reagent produced by the Developmental Studies Hybridoma Bank. It is a monoclonal antibody that binds to a specific target molecule. The core function of this antibody is to serve as a detection and analysis tool in various biological research applications.
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Lab products found in correlation
2 protocols using anti a4.1025 antibody
1
Phenotypic Characterization of pierce Mutants
2
Phenotypic Characterization of pierce Mutants
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Phenotypes were scored in live WT, pierce1, pierce2 and the double knockout mutant embryos using a stereomicroscope. Phenotypes were analyzed in embryos at different stages. Otolith counts were performed at 20–22 hpf. Curved body axis and hydrocephaly were determined at 48 and 72 hpf. Kidney cysts and edema were scored at 4–5 days post fertilization (dpf). For left–right asymmetry, immunofluorescence microscopy was carried out at 36 hpf with anti-A4.1025 antibody (Developmental Studies Hybridoma Bank) to visualize heart jogging. Heart jogging was classified as left, right, or bilateral. To evaluate significance, we used the Fisher’s Exact Test (2 × 2 matrix, two-tailed).
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