8 µl of bdEV (from a total of 40 μl RNAs) and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010) were attached according to the manufacturer’s protocol. The yield and size distribution of the small RNA libraries were assessed using the Fragment Bioanalyzer™ system with DNA 1000 chip (Agilent5067–1505). 170–230 bp libraries were selected with agarose gel cassettes (Sage Science HTG3010) on the BluePippin Size Selection System (Sage Science). Multiplexed libraries were equally pooled to 1nM and sequenced on the NovaSeq 6000 system (Illumina) with the NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina 20028401).
Htg3010
Manufactured by Sage Science
The HTG3010 is a lab equipment product from Sage Science. It is designed for high-throughput sample processing, with precise temperature control and automated liquid handling capabilities. The core function of the HTG3010 is to facilitate efficient and reproducible sample preparation across a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Novaseq 6000 sp reagent kit v1, by Illumina (3 mentions)
Novaseq 6000 system, by Illumina (3 mentions)
D plex small rna seq kit, by Diagenode (3 mentions)
D plex single indexes for set a, by Illumina (3 mentions)
Fragment bioanalyzer system with dna 1000 chip, by Agilent Technologies (3 mentions)
Mirneasy mini kit reagent, by Qiagen (2 mentions)
Zymo spin 1 column, by Zymo Research (2 mentions)
Bluepippin, by Sage Science (2 mentions)
Bluepippin size selection system, by Sage Science (1 mentions)
3 protocols using htg3010
1
Small RNA Library Preparation and Sequencing
2
Small RNA Sequencing from Extracellular Vesicles
3
Small RNA-seq of Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
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bdEV RNA was extracted by miRNeasy Mini Kit reagents (Qiagen 217004) and Zymo-Spin I Columns (Zymo Research C1003–50) according to the manufacturer’s instructions. bdEV RNA was resuspended in 40 μL Rnase-free water, and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010) according to the manufacturer’s protocol. The yield and size distribution of the small RNA libraries were assessed using the Fragment Bioanalyzer™ system with DNA 1000 chip (Agilent 5067–1505). After size selection of the libraries by agarose gel cassettes (Sage Science HTG3010) on BluePippin (Sage Science) from 170–230 bp, multiplexed libraries were equally pooled to 1 nM. and prepared for deep sequencing using the NovaSeq 6000 system (Illumina) and sequenced by NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina 20028401).
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