Oxoid campygen sachet

Manufactured by Thermo Fisher Scientific

The Oxoid CampyGen sachet is a laboratory product designed to create a microaerophilic atmosphere, which is required for the growth of Campylobacter and other microorganisms. The sachet generates a controlled environment by producing a gas mixture with reduced oxygen and increased carbon dioxide levels when activated.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using oxoid campygen sachet

Isolation and Identification of Campylobacter jejuni

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stools collected by local health departments from persons presumed to have Campylobacter jejuni were plated on Campy CVA agar plates (Thermo Fisher Scientific, Waltham, MA). Isolates previously identified and submitted by clinical laboratories were plated on trypticase soy agar plates supplemented with 5% sheep blood. Agar plates were incubated overnight at 42°C under microaerophilic conditions created in a 2.5-liter jar with 5% O₂, 10% CO₂, and 85% N₂ produced by an Oxoid CampyGen sachet (Thermo Fisher Scientific). The plates were examined at 24, 48, and 72 h for characteristic Campylobacter growth. Colonies exhibiting typical Campylobacter morphology were tested biochemically, with Campylobacter jejuni being identified based on positive oxidase, catalase, and hippurate reactions (15 (link)). Isolated colonies were archived at −80°C in a cryopreservative broth containing approximately 20 to 30 individual CryoBeads (Hardy Diagnostics, Santa Maria, CA).

Oakeson K.F., Wagner J.M., Rohrwasser A, & Atkinson-Dunn R. (2018). Whole-Genome Sequencing and Bioinformatic Analysis of Isolates from Foodborne Illness Outbreaks of Campylobacter jejuni and Salmonella enterica. Journal of Clinical Microbiology, 56(11), e00161-18.

+ Open protocol

+ Expand

Isolating and Extracting DNA from Campylobacter

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen isolates were removed from the −80°C freezer and allowed to thaw briefly. A sterile wooden stick was then used to remove approximately 5 CryoBeads (Thermo Fisher Scientific) from the freezer vial, and they were plated on a blood agar plate (Thermo Fisher Scientific) with gentle rolling. Plates were incubated overnight at 42°C under microaerophilic conditions created in a 2.5-liter jar with 5% O₂, 10% CO₂, and 85% N₂ produced by an Oxoid CampyGen sachet (Thermo Fisher Scientific). Using a sterile loop, approximately 1 μl of growth was transferred to 1 ml of sterile PBS and vortex-mixed. These cells were centrifuged at 5,000 × g for 10 min. The supernatant was removed, and the pelleted bacteria were used for DNA extraction using the DNeasy blood and tissue kit (Qiagen). For isolates that could not be recovered from frozen cultures, DNA was extracted directly from the PFGE plugs with the QIAquick gel extraction kit (Qiagen). DNA in the final eluate was quantified by using a Qubit 3.0 fluorometer (Thermo Fisher Scientific).

+ Open protocol

+ Expand

Culturing and Maintaining Helicobacter pylori

Check if the same lab product or an alternative is used in the 5 most similar protocols

A complete list of the strains, plasmids, and primers used in this study can be found in S1–S3 Tables. H. pylori was grown on Columbia blood agar (Difco) plates containing 5% (v/v) defibrinated horse blood (Hemostat Labs), 50 μg/mL cycloheximide (Sigma), 10 μg/mL vancomycin (Sigma), 5 μg/mL cefsulodin (Sigma), 2.5 U/mL polymyxin B (Sigma), 5 μg/mL trimethoprim (Sigma), 8 μg/mL amphotericin B (Fisher Scientific), 0.2% (w/v) β-cyclodextrin (Sigma), and 25 μg/mL chloramphenicol (Sigma) or 50 μg/mL kanamycin (Fisher Scientific) as needed at 37°C in a humidified 10% CO₂ incubator for 2–3 days. Overnight cultures were prepared in vented T25 flasks (Falcon) using Brucella broth (Difco) containing 10% (v/v) heat-inactivated fetal bovine serum (FBS-HI; Gibco). Flasks were incubated for 16–18 h at 37°C with shaking at 100 rpm under microaerophilic conditions using a 2.5 L gas pack jar containing an Oxoid CampyGen sachet (Thermo Fisher).

Baskerville M.J., Kovalyova Y., Mejías-Luque R., Gerhard M, & Hatzios S.K. (2023). Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection. PLOS Pathogens, 19(7), e1011526.

+ Open protocol

+ Expand

Helicobacter hepaticus Infection Model for Intestinal Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Helicobacter hepaticus was originally purchased from ATCC (#51449). Frozen stock was stored at −80°C in Brucella broth (BD) with 20% glycerol (Sigma-Aldrich). For maintenance and infection, the bacteria were grown on Brucella agar plates (BD) supplemented with 5% sheep blood (Thermo Scientific) in anaerobic jars with Oxoid CampyGen sachet (Thermo Scientific) at 37°C for 5 days. Mice were oral infected with 10⁸ CFU Helicobacter hepaticus on day 0 and 5, and 800 μg of anti-IL-10R antibody (BioXcell, clone 1B1.3A) was administered intraperitoneally per week. Tissue samples from the intestines of mice were fixed with 4% paraformaldehyde, embedded in paraffin, and 5 μm sections were stained with haematoxylin and eosin. Histopathological scoring of intestinal inflammation was performed in a blind manner according to a previous publication^{53 (link)}. Briefly, inflammation was graded on a scale from 0 to 3, including the following criteria: (i) epithelial hyperplasia and goblet cell depletion, (ii) lamina propria leukocyte infiltration, (iii) area of tissue affected, (iv) markers of severe inflammation, including crypt abscesses, sub-mucosal inflammation, and ulceration. Scores for individual criteria were added up for an overall inflammation score between 0 and 12.

Zhou L., Zhou W., Joseph A.M., Chu C., Putzel G.G., Fang B., Teng F., Lv M., Yano H., Andreasson K.I., Mekada E., Eberl G, & Sonnenberg G.F. (2022). Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation. Nature immunology, 23(2), 251-261.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!