Formalin-fixed tissue sections (3 µm) were stained with haematoxylin and eosin using standard techniques. Sections were assessed in a blinded manner using a Leica (Wetzlar, Germany) DM750 inverted light microscope captured images as described [23 (link)].
Dm750 inverted light microscope
Manufactured by Leica
Sourced in Germany
The Leica DM750 is an inverted light microscope designed for routine laboratory applications. It features a sturdy ergonomic design and provides high-quality optical performance for a variety of microscopy techniques.
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Lab products found in correlation
2 protocols using dm750 inverted light microscope
1
Histological Tissue Analysis Protocol
2
Histological Assessment of Liver and Lung Damage
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Samples of liver and lung collected from euthanised mice were fixed overnight in neutral buffered formalin (10%) (Sigma‐Aldrich). Tissues were processed using an ASP200S tissue processor (Leica, Buffalo Grove, USA) and embedded in paraffin wax. Tissues were sectioned (3 μm) using an RM2255 microtome (Leica) and stained with haematoxylin and eosin (POCD, Artarmon, Australia). Histological damage was assessed using a DM750 inverted light microscope (Leica) with the 20× objective and images were captured using Leica Application Suite Software v4.7. Liver damage was graded in a blinded fashion using a standardised grading system (using grades 0–4) as described.
60 (link) Lung damage was assessed by blinded area measurements of open alveoli space using Fiji
61 (link) and quantified as a percentage of the total lung area measured.
62 (link)
60 (link) Lung damage was assessed by blinded area measurements of open alveoli space using Fiji
61 (link) and quantified as a percentage of the total lung area measured.
62 (link)
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