Pharmaceutical pentobarbital sodium powder (CAS 57-33-0, batch 2019100735, exp. 30/12/2023) and oleic acid (CAS 112-80-1, batch 20002698002, exp. 31/10/2022) were supplied by Inresa (Bartenheim, France). Witepsol W25 (batch 19090001/A, exp. 05/2023) was supplied by Cooper (Melun, France). Deionised water was purchased from Fresenius (Sèvres, France). The High-Performance Liquid Chromatography (HPLC)-grade methanol was obtained from Carlo Erba reagents (CAS 67-56-1, Val de Reuil, France). Hydrogen peroxide 30% was supplied by Cooper (CAS 7722-84-1, Melun, France). Potassium dihydrogen phosphate (CAS 7778-77-0; KH2PO4) was purchased from Sigma-Aldrich (St. Louis, MO, USA). HCl (CAS 7647-01-0), NaOH (CAS 1310-73-2), and 1-Octanol (CAS 111-87-5) were purchased from Honeywell via a local vendor (MC2, Clermont-Ferrand, France).
1 octanol
Manufactured by Honeywell
Sourced in France, Italy
1-Octanol is an organic chemical compound with the formula CH3(CH2)7OH. It is a clear, colorless liquid with a mild, floral odor. 1-Octanol has a wide range of industrial and laboratory applications, including use as a solvent, chemical intermediate, and in the production of other compounds.
Automatically generated - may contain errors
Lab products found in correlation
Deionised water, by Fresenius (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Honeywell (1 mentions)
Sodium hydroxide (naoh), by Honeywell (1 mentions)
Calcein, by Thermo Fisher Scientific (1 mentions)
Texas red 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine, by Thermo Fisher Scientific (1 mentions)
Triethylammonium salt texas red dhpe, by Thermo Fisher Scientific (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Chloroform, by Avanti Polar Lipids (1 mentions)
Chloroform, by Honeywell (1 mentions)
Glycerol, by Honeywell (1 mentions)
2 protocols using 1 octanol
1
Formulation and Characterization of Pentobarbital Sodium
2
Lipid-Oil Vesicle Preparation with Fluorescent Marker
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To prepare the solutions, 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) as solutions in chloroform and 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DOPG) were purchased from Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red-DHPE), and calcein (>93%) were purchased from Thermo Fisher Scientific (Monza, Italy). chloroform, 1-octanol, and glycerol were acquired from Honeywell (Milan, Italy), and sucrose ultrapure was obtained from VWR-Life Science. Milli-Q water obtained using a purification system (Fulltech Instruments, Rome, Italy) was used for the preparation of all aqueous solutions.
The lipid mixture (DOPC:DOPG in a 1:1 ratio with 0.1 mol% Texas Red-DHPE) was dissolved in chloroform in a glass vial. chloroform was evaporated under a gentle stream of nitrogen, and the lipids were further dried by desiccating for at least 1 h. After adding octanol in order to achieve a final lipid concentration of 15 mg/mL, the lipid–oil solution (LO) was sonicated for 30 min and then filtered using 0.45 µm syringe filters. The internal aqueous solution (Wi) was 600 mM sucrose with 0.05 mM calcein, and the external aqueous solution (We) was 600 mM glucose.
The lipid mixture (DOPC:DOPG in a 1:1 ratio with 0.1 mol% Texas Red-DHPE) was dissolved in chloroform in a glass vial. chloroform was evaporated under a gentle stream of nitrogen, and the lipids were further dried by desiccating for at least 1 h. After adding octanol in order to achieve a final lipid concentration of 15 mg/mL, the lipid–oil solution (LO) was sonicated for 30 min and then filtered using 0.45 µm syringe filters. The internal aqueous solution (Wi) was 600 mM sucrose with 0.05 mM calcein, and the external aqueous solution (We) was 600 mM glucose.
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