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Hrp labelled sheep anti mouse antiserum

Manufactured by GE Healthcare
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HRP-labelled sheep anti-mouse antiserum is a secondary antibody used in immunoassays and immunohistochemistry. It is produced by immunizing sheep with mouse immunoglobulins and labeling the resulting antibodies with horseradish peroxidase (HRP). The HRP label allows for the detection and visualization of target antigens recognized by mouse primary antibodies.

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Lab products found in correlation

Control igg4, by Merck Group (2 mentions) Mouse monoclonal anti human ige antiserum, by BD (2 mentions) Mab102.1f10, by Merck Group (1 mentions)

Close spelling variants of this product

HRP anti-mouse

2 protocols using hrp labelled sheep anti mouse antiserum

1

Quantifying IgE Inhibition by Monoclonal Antibody

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ELISA plates were coated with allergens (0.1 μg/well) and pre‐incubated with mAb102.1F10 (2 μg/well) or with control IgG4 (2 μg/well; Sigma‐Aldrich). Next, plates were incubated with sera from Phl p 7‐allergic patients or a nonallergic subject (diluted 1 : 5). Bound IgE antibodies were detected with a mouse monoclonal anti‐human IgE antiserum (PharMingen) followed by the HRP‐labelled sheep anti‐mouse antiserum (GE Healthcare). All determinations were performed in duplicates and mean values were calculated. Percentage inhibitions were determined as follows: 100‐(OD bound IgE after pre‐incubation with mAb102.1F10 * 100/OD bound IgE after pre‐incubation with control IgG4) (Fig. 3).
Gadermaier E., James L.K., Shamji M.H., Blatt K., Fauland K., Zieglmayer P., Garmatiuk T., Focke‐Tejkl M., Villalba M., Beavil R., Keller W., Valent P., Durham S.R., Gould H.J., Flicker S, & Valenta R. (2015). Epitope specificity determines cross‐protection of a SIT‐induced IgG4 antibody. Allergy, 71(1), 36-46.
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2

Allergen-Specific IgE Inhibition Assay

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ELISA plates were coated with allergens (0.1 μg/well) and pre-incubated with mAb102.1F10 (2 μg/well) or with control IgG4 (Sigma-Aldrich, 2 μg/well). Next, plates were incubated with sera from Phl p 7-allergic patients or a non-allergic subject (diluted 1:5). Bound IgE antibodies were detected with a mouse monoclonal anti-human IgE antiserum (PharMingen) followed by the HRP-labelled sheep anti-mouse antiserum (GE Healthcare). All determinations were performed in duplicates and mean values were calculated. Percent inhibitions were determined as follows: 100-(OD bound IgE after pre-incubation with mAb102.1F10 * 100/OD bound IgE after pre-incubation with control IgG4) (Fig. 3).
Gadermaier E., James L.K., Shamji M.H., Blatt K., Fauland K., Zieglmayer P., Garmatiuk T., Focke‐Tejkl M., Villalba M., Beavil R., Keller W., Valent P., Durham S.R., Gould H.J., Flicker S, & Valenta R. (2015). Epitope specificity determines cross‐protection of a SIT‐induced IgG4 antibody. Allergy, 71(1), 36-46.
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