A single dose of histamine (100 µM, Sigma Aldrich, Missouri, USA) or amthamine (100 µM, H2 agonist, Sigma Aldrich, Missouri, USA) was applied to the tissue after a 30-minute equilibration period. Baseline tension, amplitude and spontaneous phasic contractions of U&LP were measured before the application of the agonist and 2 minutes after. The baseline tension for detrusor was measured at the same time-points as used for U&LP.
Amthamine
Manufactured by Merck Group
Sourced in United States
Amthamine is a laboratory equipment product manufactured by Merck Group. It is designed for use in various scientific and research applications. The core function of Amthamine is to provide a reliable and precise tool for conducting laboratory experiments and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Histamine, by Merck Group (2 mentions)
Isobutylmethylxanthine, by Merck Group (2 mentions)
Forskolin, by Merck Group (2 mentions)
3h camp, by PerkinElmer (2 mentions)
Cimetidine, by Merck Group (2 mentions)
Famotidine, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Natocor (1 mentions)
3 h tiotidine, by PerkinElmer (1 mentions)
Pd98059, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Bromodeoxyuridine (brdu), by Merck Group (1 mentions)
Optiphase hisafe 3 scintillation cocktail, by PerkinElmer (1 mentions)
L 748 337, by Merck Group (1 mentions)
Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions)
Fluorobrite dmem, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Collagenase type 4, by Worthington (1 mentions)
3h cgp12177, by PerkinElmer (1 mentions)
5 protocols using amthamine
1
Histamine and H2 Agonist Effects on Urinary Bladder
2
Radioligand binding assay protocols
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Cell culture medium, antibiotics, isobutylmethyl xanthine (IBMX), cAMP, bovine serum albumin (BSA), cycloheximide, amthamine, famotidine, forskolin, and PD98059 were obtained from Sigma Chemical Company (St. Louis, MO, USA). Tiotidine were from Tocris Cookson Inc. (Ballwin, MO, USA). [3H]cAMP, and [3H]tiotidine were purchased from Perkin Elmer Life Sciences (Boston, MA, USA). Fetal calf serum was from Natocor (Argentina). Other chemicals used were of analytical grade and obtained from standard sources.
3
Investigating H2R-Axin2 Interaction in OGD-Reperfusion
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Cultured human embryonic kidney (HEK) 293 cells transfected with Flag-H2R and GFP-Axin2 plasmids were exposed to 2 h OGD and 24 h reperfusion with normal culture medium. To investigate whether interaction of H2R with Axin2 was ligand-independent, histamine (10 µM; Sigma-Aldrich), cimetidine (10 µM; Sigma-Aldrich) or cimetidine combined with amthamine (10 µM; Sigma-Aldrich) was added in HEK 293 cells during reperfusion of OGD.
4
Detailed Biochemical Assays and Antibody Sourcing
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Cell culture medium, antibiotics, isobutyl methylxanthine (IBMX), cAMP, bovine serum albumin (BSA), BRL37344, forskolin, L748337, amthamine, bromodeoxyuridine, holo-transferrin (human), insulin (bovine pancreas) pertussis toxin, and protease inhibitors were obtained from Sigma Chemical Company (St. Louis, MO). Dithiobis(succinimidyl propionate) (DSP) was from Pierce (Rockford, USA). FluoroBrite DMEM from Gibco was purchased from TermoFisher Scientific (New York, USA). [3H]cAMP, [3H](-)-CGP-12177 and Optiphase HiSafe3 scintillation cocktail were purchased from Perkin Elmer Life Sciences (Boston, MA). Foetal bovine serum was from Natocor (Córdoba, Argentina). Collagenase (type IV) was obtained from Worthington, (Lakewood, NJ). Other chemicals used were of analytical grade and obtained from standard sources.
Protein A/G Plus-Agarose (catalogue#SC-2003/Lot#J0118/Santa Cruz Biotechnology, CA)
The following primary antibodies were purchased from Santa Cruz Biotechnology, CA:
The following secondary antibodies were purchased from Vector Laboratories, CA:
Primers listed below were obtained from Genbiotech Oligos, BA:
Protein A/G Plus-Agarose (catalogue#SC-2003/Lot#J0118/Santa Cruz Biotechnology, CA)
The following primary antibodies were purchased from Santa Cruz Biotechnology, CA:
The following secondary antibodies were purchased from Vector Laboratories, CA:
Primers listed below were obtained from Genbiotech Oligos, BA:
5
Neonatal HI and LPC Injury Models
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Mice were intraperitoneally injected with saline, H2R antagonist cimetidine (20 mg/kg/d; Sigma-Aldrich), zolantidine (10 mg/kg/d; Sigma-Aldrich), or cimetidine combined with H2R agonist amthamine (5 mg/kg/d; Sigma-Aldrich) after neonatal HI surgery. Mice were intraperitoneally injected with saline or H2R antagonist cimetidine (20 mg/kg/d; Sigma-Aldrich) after LPC microinjection in a two-site LPC injection model.
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