Murine colorectal carcinoma cell line CT26 and murine triple negative breast cancer cell line 4T1 were grown in RPMI-based media (ATCC, Manassas, VA, USA), supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Sartorius, Beit HaEmek, Israel). The human pancreatic adenocarcinoma cell line, Panc 02.03, was grown in RPMI-based media (ATCC) supplemented with 15% fetal bovine serum, 1% penicillin-streptomycin (Sartorius), and 10 units/mL human recombinant insulin (Merck, Rahway, NJ, USA). All cell lines were purchased from the American Type Culture Collection (ATCC) local distributor, Sartorius (Beit HaEmek, Israel).
Rpmi based media
Manufactured by American Type Culture Collection
Sourced in United States
RPMI-based media is a type of cell culture media formulated to support the growth and maintenance of a variety of cell types. It provides the necessary nutrients, growth factors, and other components required for cell proliferation and survival in in vitro cell culture applications.
Automatically generated - may contain errors
2 protocols using rpmi based media
1
Culturing Murine and Human Cancer Cell Lines
2
Culturing Mouse Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse CT26.WT colon, 4T1 breast and LLC-1 Lewis lung carcinoma cancer cell lines were purchased from an American Type Culture Collection (ATCC) local distributor, Sartorius (Beit Haemek, Israel). CT26 and 4T1 cell lines were grown in RPMI-based media (ATCC) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin (Sartorius). LLC-1 cells were grown in DMEM medium (ATCC) supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin (Sartorius).
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