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Coolpix p520

Manufactured by Nikon
Sourced in Japan

The Nikon Coolpix P520 is a digital camera with a 42x optical zoom lens. It has a 16.1-megapixel CMOS sensor and can record full HD 1080p video. The camera is equipped with built-in Wi-Fi connectivity for wireless image transfer and sharing.

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5 protocols using coolpix p520

1

Measurement of Cervicothoracic Angle for Postural Analysis

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The measurement of cervicothoracic angle is a clinical measurement [22 (link)] that indicates the neck posture and is defined as the angle between C7 spinal process, the middle of the ear tragus, and the horizontal level. The cervicothoracic angle measures the angle produced between the horizontal plane of the picture and the line of C7 spinal process and the tragus of the ear for the traction of the head. The digital photo pictures were taken with a Nikon Coolpix P520 and the analyses of the angles were done with digital protractor in Photoshop software. The reduced value of this angle is an indication for forward head posture [25 (link)]. For this clinical measurement, a digital camera Nikon Coolpix P520 was placed in a stable base in the kinesiotherapy lab of the institution, 3 metres away from each patient calibrated in a specific place for the whole duration of the study. The participant position for measurement was standardised supported sitting and participants were asked to stare at a stable marker placed in the wall, ahead of them. Reliability of the cervicothoracic angle was measured in 15 healthy subjects with digital protractor with Photoshop CS3 software (3 consecutive days) and yielded excellent intraexaminer reliability ICC = 0.91.
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2

Photography and Terminology of Orthoptera Specimens

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Photography. Specimens were photographed using Canon EOS 800D with 100 mm macro lens, and partial images were stacked using Photoshop CS6. Photographs of the habitat were made using Nikon Coolpix P520.
Terminology. Morphological terminology and measurements follow Zheng (2005) , Tumbrinck (2014) and Muhammad et al. (2018) (link). Measurements are given in millimeters (mm).
Depository. Type and voucher specimens are deposited in the Specimen Room of the School of Life Sciences, Huaibei Normal University (HNU), Huaibei, Anhui Province, China.
Taxonomy. Taxonomy follows Cigliano et al. (2020) (= Orthoptera Species File).
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3

3D Photogrammetry for Ichnological Studies

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High-resolution digital photogrammetry was undertaken to achieve a more objective representation of track three-dimensional morphology, according to a recently described standard protocol for ichnological studies (Falkingham et al., 2018 (link)). To model the studied specimens, the software package Agisoft PhotoScan Pro (Educational License), which enables creating 3D textured meshes by means of semi-automatic processing of images (Mallison & Wings, 2014 ), was used.
The images selected for the photogrammetric process were acquired using a Nikon Coolpix P520 camera with 4.3–7.6 focal length, resolution 4,896 × 3,672 and pixel size ranging from 1.25 × 1.25 µm and 1.27 × 1.27 µm. Main processing parameters are reported in Table 1. In order to correctly scale the calculated model, a metric reference marker was applied on the surface. Three-dimensional models were converted to colour topographic profiles using the software Paraview (version 5.4.1).
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4

Cordyceps Species Identification and Characterization

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Wireworm-infecting species of Cordyceps s.l. were collected from south-western China and the Russian Far East. Specimens were placed in plastic boxes and carried to the laboratory for further study. The macro-characteristics and ecology were photographed using a Nikon Coolpix P520 camera in the field. Specimens were examined and photographed using an Optec SZ660 stereo dissecting microscope and a Nikon Eclipse 80i compound microscope connected with a Canon EOS 600D camera. Microscopic measurements were made using Tarosoft (R) Image Framework software. Images were processed using Adobe Photoshop CS v. 8.0.1 (Adobe Systems Incorporated, San Jose, California, USA). Voucher specimens are deposited in the Fungarium of the Centre of Excellence in Fungal Research, Mae Fah Luang University (MFLU), Chiang Rai, Thailand and the Herbarium of Guizhou University (GACP), Guiyang, China.
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5

Annotating Fuyu Persimmon Seed Count

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A total of 599 fully matured 'Fuyu' persimmon fruits were harvested from four independent trees (38, 57, 58, and 92 years old) in late November 2018, at Gifu Prefectural Agricultural Technology Center, Gifu, Japan (N35.441721, E136.699894). The fruits were placed on a gray-background sheet for photos from the fruit apex side using a digital camera (COOLPIX P520; Nikon Corporation, Japan). The LED positional light (KANSAI RACK, Japan) was set just above the camera and the fruits. The distances from the camera and the positional light to the fruits were 40 cm and 60 cm, respectively. The detailed settings of the camera were Fvalue of F/4, exposure time of 1/50 second, ISO-400, default white balance (auto), and the size of the image was 1600 × 1200 pixels. The images were taken in a dim room to avoid the influence of external light. The 'Fuyu' fruits were dissected to visually annotate seed numbers (n = 0-8) after taking the photos.
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