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Truseq amplicon 2.0 basespace app workflow

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The TruSeq Amplicon 2.0 BaseSpace app workflow is a software tool that enables the analysis of targeted sequencing data generated using the TruSeq Amplicon assay. The app provides a streamlined workflow for data processing, alignment, and variant calling.

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Lab products found in correlation

Ion torrent s5, by Thermo Fisher Scientific (2 mentions) Truseq custom amplicon next generation sequencing gene panel, by Illumina (2 mentions)

Spelling variants of this product

BaseSpace (241 citations) TruSeq 2 (9 citations) TruSeq workflow (3 citations)

2 protocols using truseq amplicon 2.0 basespace app workflow

1

Myeloid Malignancies Mutational Profiling

Cited 1 time
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The mutational profile of 32 recurrently mutated genes in myeloid malignancies was determined using an Illumina TruSeq Custom Amplicon next-generation sequencing gene panel (Supplementary Table 2) and the TruSeq Amplicon 2.0 BaseSpace app workflow [51 (link)] (Illumina, San Diego, CA, USA). 31 inositide-specific point mutations and small indels (Supplementary Table 3) were examined using the Ion Torrent S5 with an Ion AmpliSeq™ On-demand Panel (Thermo Fisher Scientific, Whaltam, MA, USA). Sequencing alignment was viewed by the Integrative Genomics Viewer Software (Broad Institute, Cambridge, MA, USA) using the Human Genome Build 19 (Hg19) as reference [52 (link)]. Further details can be found in the Supplementary Information.
Follo M.Y., Pellagatti A., Armstrong R.N., Ratti S., Mongiorgi S., De Fanti S., Bochicchio M.T., Russo D., Gobbi M., Miglino M., Parisi S., Martinelli G., Cavo M., Luiselli D., McCubrey J.A., Suh P.G., Manzoli L., Boultwood J., Finelli C, & Cocco L. (2019). Response of high-risk MDS to azacitidine and lenalidomide is impacted by baseline and acquired mutations in a cluster of three inositide-specific genes. Leukemia, 33(9), 2276-2290.
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2

Mutational Profiling of Myeloid Malignancies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The mutational profile of 32 recurrently mutated genes in myeloid
malignancies was determined using an Illumina TruSeq Custom Amplicon
next-generation sequencing gene panel (Supplementary Table 2) and the TruSeq Amplicon 2.0
BaseSpace app workflow 51 (link) (Illumina, San
Diego, CA, USA). 31 inositide-specific point mutations and small indels (Supplementary Table 3)
were examined using the Ion Torrent S5 with an Ion AmpliSeq™ On-demand
Panel (Thermo Fisher Scientific,Whaltam, MA, USA). Sequencing alignment was
viewed by the Integrative Genomics Viewer Software (Broad Institute, Cambridge,
MA, USA) using the Human Genome Build 19 (Hg19) as the reference 52 (link). Further details can be found in the
Supplementary
Information.
Follo M.Y., Pellagatti A., Armstrong R.N., Ratti S., Mongiorgi S., De Fanti S., Bochicchio M.T., Russo D., Gobbi M., Miglino M., Parisi S., Martinelli G., Cavo M., Luiselli D., McCubrey J.A., Suh P.G., Manzoli L., Boultwood J., Finelli C, & Cocco L. (2019). Response of high-risk MDS to azacitidine and lenalidomide is impacted by baseline and acquired mutations in a cluster of three inositide-specific genes. Leukemia, 33(9), 2276-2290.
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