The following antibodies were used in this study: α-synuclein monoclonal antibody (BD Biosciences; #610787, San Diego, CA), α-synuclein monoclonal antibody #274, phosphorylated α-synuclein polyclonal antibody (Abcam, ab59264; Cambridge, MA), GFP (c-terminus) polyclonal antibody (IMGENEX, #5127A; San Diego, CA), GFP (N-terminus) polyclonal antibody (Cell signaling Technology, #2555; Beverly, MA), GFP (N-terminus) monoclonal antibody (Abcam, ab127417), GCase monoclonal antibody 8E4 (from J. Barranger, University of Pittsburgh), GCase polyclonal antibody (Sigma, G4171; St. Louis, MO), p62 monoclonal antibody (BD Transduction laboratories, #c2384-0B; Swampscott, MA), ubiquitin polyclonal antibodies (Dako; Glostrup, Denmark and Chemicon; Temecula, CA), and β-actin monoclonal antibody (Sigma). Fluorescence dye-conjugated goat anti-rabbit IgG was purchased from Jackson Immunoresearch Laboratories (West Grove, PA). The Q tracker 858 cell labeling kit was purchased from Invitrogen (Carlsbad, CA).
Gcase monoclonal antibody 8e4
Manufactured by Merck Group
Sourced in United States
The GCase monoclonal antibody 8E4 is a laboratory reagent used for the detection and quantification of the glucocerebrosidase (GCase) enzyme. It is a mouse-derived monoclonal antibody that specifically binds to the GCase protein. The GCase monoclonal antibody 8E4 can be utilized in various analytical techniques, such as Western blotting, ELISA, and immunohistochemistry, to study the expression and distribution of the GCase enzyme in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
β actin monoclonal antibody, by Merck Group (2 mentions)
Ubiquitin polyclonal antibodies, by Agilent Technologies (2 mentions)
α synuclein monoclonal antibody, by BD (1 mentions)
Lysotracker red dnd 99 l 7528, by Thermo Fisher Scientific (1 mentions)
Fluorescein conjugated dextran, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated goat anti rabbit igg h l, by Bio-Rad (1 mentions)
2 protocols using gcase monoclonal antibody 8e4
1
Antibody Panel for Parkinson's Research
2
Antibody and Fluorescence Reagent Protocol
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The following antibodies were used in this study: GCase monoclonal antibody 8E4 (from J Barranger, University of Pittsburgh; 1:1000), GCase polyclonal antibody (G4171, Sigma-Aldrich, St Louis, MO, USA; 1:1000), β-actin monoclonal antibody (Sigma-Aldrich; 1:10 000), p62 monoclonal antibody (c2384-0B, BD Transduction Laboratories, Swampscott, MA, USA; 1:1000), ubiquitin polyclonal antibodies (Dako, Glostrup, Denmark and Chemicon, Temecula, CA, USA; 1:1000), α-synuclein monoclonal antibody (610787, BD Biosciences; 1:1500), α-synuclein monoclonal antibody Ab274 (1:1500),17 (link) α-synuclein monoclonal antibody Ab62 (1:1000),17 (link) Horse radish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG; H+L) (172–1011, Bio-Rad Laboratories, Hercules, CA, USA; 1:3000) and HRP-conjugated goat anti-rabbit IgG (H+L) (Bio-Rad Laboratories; 1:3000).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
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