Total mRNA from parental and CDVR cells was isolated with the Quickprep mRNA purification kit (Amersham Biosciences) and converted to cDNA with the first-strand cDNA synthesis kit (GE Healthcare). The entire cDNA from each selected gene was amplified by PCR using specific primers. The PCR products were purified using PCR product purification kit (Roche) and directly sequenced using a cycle-sequencing kit (Dyenamic dye terminator kit; Amersham Biosciences), specific primers targeting both strands of the specific gene, and a capillary DNA sequencing system (MegaBACE 500; Amersham Biosciences). The data were assembled and compared to the DNA sequences obtained from reference sequences using Vector NTI software (Invitrogen). The primers used for the genotyping of UMP/CMPK1 and 2, and HPV oncogenes E6 and E7 are listed in Table S2 .
Quickprep mrna purification kit
Manufactured by Cytiva
The Quickprep mRNA purification kit is a laboratory equipment product designed for the rapid and efficient isolation of mRNA from a variety of biological samples. The kit utilizes a combination of proprietary reagents and protocols to selectively capture and purify mRNA molecules from complex mixtures, enabling researchers to obtain high-quality mRNA samples for downstream applications.
Automatically generated - may contain errors
2 protocols using quickprep mrna purification kit
1
Sequencing and Genotyping of Genes
2
Sensorineural Hearing Loss PEX26 RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly(A)+ RNA was obtained from fibroblasts from a proband with sensorineural hearing loss by a QuickPrep mRNA purification kit (Amersham Biosciences). RT-PCR of PEX26 was performed with a pair of forward primer 1F (5′-AAGAGCGATTCTTCGACC-3′ at amino acid residues 4–21 and reverse primer 1R (5′-GTCACGGATGCGGAGCTG-3′ at 898–915 (Matsumoto et al. 2003a (link)). PCR products were directly sequenced.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!