In order to collect the thermographic data for this build a mid-wave IR camera—the FLIR 7600—was placed outside of the ARCAM vacuum chamber and aimed at the build plate through a leaded glass viewing window that was used to protect the camera’s sensor from radiation produced by the electron beam. Due to the space limitations inside of the ARCAM machine, the camera also had to be placed at an angle of 22 degrees to the build plate. To prevent metal vapor produced during the build from adhering to the viewing window, a roll of thin Kapton film was slowly scrolled in front of the viewing window to carry away the vapor as it collects on the film. By using this Kapton film to protect the window, it prevents any material build up from diminishing the accuracy of the IR images that were collected. The camera used a 25mm lens without a filter, the integration time was set at 86.4 μs, and the frame rate was set at 10 Hz. After the collection of all the thermographic imaging of the build, the data was then imported into FLIR’s proprietary imaging software ResearchIR for initial analysis and compilation of the data. Since the factory calibrations of the camera were not accurate for monitoring this build, the measurements collected were converted from the factory settings to the raw image intensity units, called counts in the software.
Researchir
Manufactured by Teledyne
ResearchIR is a high-performance infrared imaging system developed by Teledyne. It provides advanced thermal imaging capabilities for scientific and research applications. The core function of ResearchIR is to capture and analyze infrared radiation, enabling precise temperature measurements and thermal analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using researchir
1
In-situ Thermographic Monitoring of ARCAM Builds
2
Thermal Imaging of Chemogenetic Manipulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Temperature measurements started 4 hours into the dark phase. Prior to baseline temperature measurements, food, water, and tissues were removed from the home cage. To measure temperature, mice were placed in a smaller cage, and a movie was made using a thermal camera (Flir T420; FLIR Systems, Inc.) and the accompanying software program ResearchIR (version 4.40.6.24, 64 bit; FLIR Systems). Fifteen and thirty minutes after removal, baseline temperature was recorded. Mice were then injected with either saline or CNO, and temperature was recorded 30, 60, and 90 minutes after injections. In between movies, mice were placed back in their home cage. ResearchIR was used to determine eye temperature by directing a 3 × 3‐sized pixel toward the region of the eyes with the least standard deviation with mice standing on their hind legs and looking upward. Temperature is displayed as a change in temperature from baseline (temperature − average of baseline temperatures).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!