BTK occupancy in splenocytes was measured as previously described.20 (link) Protein expression was determined using standardized protocols with monoclonal antibodies reactive with the following proteins: BTK (clone 3533; Cell Signaling, Danvers, MA, USA; 1:1000), CXCR4 (clone 2074; Abcam, Cambridge, UK; 1:500), phospho-CXCR4 (clone 74012, Abcam; 1:500), PIM-1 (clone EP2645Y; Abcam; 1:10 000), GRK6 (clone D1A4, Cell Signaling; 1:1000), TCL1 (clone 4042; Cell Signaling; 1:1000) and β-actin (clone 8227; Abcam; 1:5000). Phosphorylation states of multiple PKC isoforms were tested by the phospho-PKC Antibody Sample Kit (Cell Signaling, catalog number 9921). Fluorescently stained protein bands were quantified using Molecular Dynamics ImageQuant 5.2 software (GE Healthcare, Waukesha, WI, USA).
Molecular dynamics imagequant 5
Manufactured by GE Healthcare
Sourced in United States
The Molecular Dynamics ImageQuant 5.2 software is a laboratory equipment product designed for image analysis and quantification. It provides tools for processing and analyzing digital images, primarily intended for use in scientific research and applications.
Automatically generated - may contain errors
Lab products found in correlation
Btk clone 3533, by Cell Signaling Technology (2 mentions)
Cxcr4 clone 2074, by Abcam (2 mentions)
Phospho cxcr4 clone 74012, by Abcam (2 mentions)
Pim 1 clone ep2645y, by Abcam (2 mentions)
Grk6 clone d1a4, by Cell Signaling Technology (2 mentions)
Tcl1 clone 4042, by Cell Signaling Technology (2 mentions)
β actin clone 8227, by Abcam (2 mentions)
Phospho pkc antibody sample kit, by Cell Signaling Technology (2 mentions)
2 protocols using molecular dynamics imagequant 5
1
Quantification of Immune Signaling Proteins
2
Quantification of BTK and CXCR4 Signaling Pathways
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