Kgm 2 sf

Human hypopharyngeal primary cells (HHPC) were obtained from Celprogen Inc. CA, USA. The HHPC were plated in non-coated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA, USA), at 37°C in humidified air and 5% CO₂. Cells were sub-cultured and media were gradually replaced by Serum Free Media (Celprogen Inc. CA, USA).
We also established a telomerase-immortalized human hypopharyngeal cell line (HHK), by expression of hTERT, extending its life span without altering the characteristic phenotypic properties of the cells, as previously described [10 (link),33 (link)]. HHK, were grown in keratinocyte serum free basal medium (KGM-2 SF, Gibco^®) supplemented by L-Glutamine, BPE, hEGF and gentamicin (Gibco^®), at 37°C in humidified air and 5% CO₂.

We used human hypopharyngeal primary cells (HHPC) from Celprogen Inc. CA, USA. The HHPC were plated in non-coated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA, USA), at 37°C in humidified air and 5% CO2. The HHPC were sub-cultured and media were gradually replaced by Serum Free Media (Celprogen Inc. CA, USA), and passed after reaching ∼90% confluence, using 0.05% trypsin-EDTA (Gibco^®, NY, USA).
We also established a telomerase-immortalized human hypopharyngeal keratinocytes (HHK), by expression of hTERT, extending its life span without altering the characteristic phenotypic properties of the cells, as previously described [20 (link)]. HHK, were grown in keratinocyte serum free basal medium (KGM-2 SF, Gibco^®, NY, USA) supplemented by L-Glutamine, BPE, hEGF and gentamicin (Gibco^®, NY, USA), at 37°C in humidified air and 5% CO2.