Anti collagene type 1

Manufactured by Rockland Immunochemicals

Anti-collagene type I is a laboratory reagent used to detect and quantify type I collagen in samples. It functions as a specific antibody that binds to type I collagen, enabling its identification and measurement.

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Lab products found in correlation

Anti β actin, by Merck Group (2 mentions) Anti α sma, by Merck Group (2 mentions) Anti acetyl lysine, by Cell Signaling Technology (1 mentions) Anti rna polymerase 2, by Santa Cruz Biotechnology (1 mentions) Anti smad3, by Abcam (1 mentions) Anti sirt1, by Abcam (1 mentions) Anti pias1, by Santa Cruz Biotechnology (1 mentions) Anti pias2, by Santa Cruz Biotechnology (1 mentions) Anti pias3, by Santa Cruz Biotechnology (1 mentions) Anti pias4, by Merck Group (1 mentions) Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions) Anti p65, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti collagene type 1

Western Blot Analysis of ECM and Signaling

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Whole cell lysates were obtained as previously described41 (link). Western blot analyses were performed with anti-collagene type I (Rockland), anti-α-SMA, anti-β-actin, anti-PIAS4 (Sigma), anti-PIAS1, anti-PIAS2, anti-PIAS3, anti-RNA polymerase II (Santa Cruz), anti-acetyl lysine (Cell Signaling Tech), anti-SMAD3, and anti-SIRT1 (Abcam) antibodies.

Sun L., Fan Z., Chen J., Tian W., Li M., Xu H., Wu X., Shao J., Bian Y., Fang M, & Xu Y. (2016). Transcriptional repression of SIRT1 by protein inhibitor of activated STAT 4 (PIAS4) in hepatic stellate cells contributes to liver fibrosis. Scientific Reports, 6, 28432.

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Immunoprecipitation and Western Blot Analysis

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Whole cell lysates and nuclear proteins were obtained as previously described^[19 (link)^]. Specific antibodies or pre-immune IgGs (P.I.I.) were added to and incubated with cell lysates overnight before being absorbed by Protein A/G-plus Agarose beads (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Precipitated immune complex was released by boiling with 1X SDS electrophoresis sample buffer. Western blot analyses were performed with anti-collagene type I (Rockland), anti-α-SMA, anti-β-actin (Sigma), anti-Aggf1, or anti-p65 (Santa Cruz Biotechnology) antibodies.

Xu W., Zeng S., Li M., Fan Z, & Zhou B. (2017). Aggf1 attenuates hepatic inflammation and activation of hepatic stellate cells by repressing Ccl2 transcription. Journal of Biomedical Research, 31(5), 428-436.

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