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Immobilon western blotting kit

Manufactured by Merck Group
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The Immobilon Western blotting kit is a product from Merck Group designed for protein detection and analysis. The kit provides the necessary components for performing Western blot analysis, a widely used technique in molecular biology and biochemistry. The core function of this kit is to enable the transfer of separated proteins from a gel to a membrane, facilitating their subsequent detection and quantification.

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Lab products found in correlation

Anti sirt1 sc 15404, by Santa Cruz Biotechnology (2 mentions) Anti flag antibody f1804, by Merck Group (2 mentions) Anti phospho p65 s536 3031 antibodies, by Cell Signaling Technology (2 mentions) Nitrocellulose membrane, by GE Healthcare (2 mentions) Anti gapdh 5174, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti ykl40, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Protease inhibitor mixture, by Roche (1 mentions) Anti stat3 antibody, by Cell Signaling Technology (1 mentions) Anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Anti p50, by Santa Cruz Biotechnology (1 mentions) Anti relb, by Santa Cruz Biotechnology (1 mentions) Anti p65, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Immobilon (435 citations) Immobilon Western (255 citations) Immobilon Western kit (70 citations) Immobilon kit (10 citations) Immobilon ECL Western Blotting Detection Kit Reagent (3 citations) Immobilon Western blotting detection kit (2 citations)

4 protocols using immobilon western blotting kit

1

Western Blot Analysis of Cell Signaling

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The cells were lysed in 10 mM Tris (pH 7.4), 150 mM sodium chloride, 1 mM EDTA, 0.5% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and protease inhibitor mixture (Roche Applied Science). Samples were separated using SDS–PAGE and transferred onto nitrocellulose membranes. The anti-β-tubulin (sc-9104), anti-RelB (sc-226), anti-p65 (sc-372), anti-p105/p50 (sc-8414), anti-IκBα (sc-371), and anti-SIRT1 (sc-15404) antibodies (Santa Cruz Biotechnology); anti-Lamin A/C (2032), anti-myc (2276), and anti-p52 (4882) (Cell Signaling); anti-YY1 (A302-779A) (Bethyl Laboratories), and anti-flag (F1804) antibodies (Sigma-Aldrich) were used. Antigen–antibody complexes were visualized by ECL using Immobilon Western blotting kit (Millipore).
Waters M.R., Gupta A.S., Mockenhaupt K., Brown L.N., Biswas D.D, & Kordula T. (2019). RelB acts as a molecular switch driving chronic inflammation in glioblastoma multiforme. Oncogenesis, 8(6), 37.
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2

Western Blot Analysis of NF-κB Signaling

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Cells were lysed in 10 mM Tris (pH 7.4), 150 mM sodium chloride, 1 mM EDTA, 1% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and Pierce protease inhibitor mixture. Samples were separated on a 10% gel, and transferred onto nitrocellulose membranes (GE Healthcare). Anti-β-tubulin (sc-9104), anti-RelB (sc-226), anti-p65 (sc-372), anti-p105/p50 (sc-8414), anti-IκBα (sc-371), and anti-SIRT1 (sc-15404) antibodies (Santa Cruz Biotechnology); anti-Lamin A/C (2032), anti-myc (2276) anti-GAPDH (5174), and anti-phospho-p65(S536) (3031) antibodies (Cell Signaling); anti-flag (F1804) antibodies (Sigma-Aldrich); and anti-pRelB(S472) antibodies were from CliniSciences (NB-22-1004) (Nanterre, France). Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
Gupta A.S., Waters M.R., Biswas D.D., Brown L.N., Surace M.J., Floros C., Siebenlist U, & Kordula T. (2019). RelB controls adaptive responses of astrocytes during sterile inflammation. Glia, 67(8), 1449-1461.
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3

Western Blot Analysis of NF-kB Pathway

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Cells or ground flash frozen tissue were lysed in 10 mM Tris (pH 7.4), 150 mM sodium chloride, 1 mM EDTA, 1% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and Pierce protease inhibitor mixture. Samples were separated on a 10% gel and transferred onto nitrocellulose membranes (GE Healthcare). Anti-β-tubulin (sc-9104), anti-RelB (sc-226), anti-p65 (sc-372) antibodies (Santa Cruz Biotechnology); anti-GAPDH (5174); and anti-phospho-p65(S536) (3031) antibodies (Cell Signaling) were used. Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
Gupta A.S., Biswas D.D., Brown L.S., Mockenhaupt K., Marone M., Hoskins A., Siebenlist U, & Kordula T. (2019). A detrimental role of RelB in mature oligodendrocytes during experimental acute encephalomyelitis. Journal of Neuroinflammation, 16, 161.
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4

Western Blot Analysis of Protein Expression

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The cells were lysed in 10 mM Tris, pH 7.4, 150 mM sodium chloride, 1 mM EDTA, 0.5% Nonidet P-40, 1% Triton X-100, 1 mM sodium orthovanadate, 0.2 mM PMSF, and protease inhibitor mixture (Roche Applied Science, Indianapolis, IN). Samples were separated using SDS-PAGE and transferred onto nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany). The anti-YKL-40, anti-RelB, anti-p65, anti-p50, anti-β-tubulin, and anti-GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) and anti-Stat3 antibody was obtained from Cell Signaling Technology (Danvers, MA). Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore, Temecula, CA).
Bhardwaj R., Yester J.W., Singh S.K., Biswas D.D., Surace M.J., Waters M.R., Hauser K.F., Yao Z., Boyce B.F, & Kordula T. (2015). RelB/p50 complexes regulate cytokine-induced YKL-40 expression. Journal of immunology (Baltimore, Md. : 1950), 194(6), 2862-2870.
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