Chromozym TRY is a laboratory reagent used for the quantitative determination of trypsin-like enzymes. It is a chromogenic substrate that, when cleaved by trypsin-like enzymes, releases a colored product that can be measured spectrophotometrically.
Example 4
The activity of trypsin was determined using Chromozym TRY (Roche Diagnostics GmbH) in 100 mM Tris pH 8.0, 20 mM CaCl2 at 25° C. Chromozym TRY is substrate (Carbobenzoxy-L-valyl-L-glycyl-L-arginine-4-nitranilide acetate) for the reliable photometrical determination of activity of proteases which hydrolyze peptides at the carboxylic side of arginine (trypsin, endoproteinase Arg-C and others). Photometric measurement was carried out at 405 nm.
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