Human absorbed goat anti rabbit igg pe or igg alexa

Example 2

The inventors analyzed 163 human B-cell lymphomas for JAM-C expression (FIG. 3). The expression of JAM-C in B-cell lymphomas revealed a disease-specific pattern distinguishing JAM-Cpos from JAM-Cneg B-cell lymphomas. Therefore, JAM-C expression clearly allows the distinction between JAM-Cpos lymphomas (MZBL, MCL, HCL) and JAM-Cneg lymphomas (CLL, FL).

To determine JAM-C expression, cell samples were incubated with JAM-C rabbit antiserum at a dilution of 1:2000 after incubation with normal human serum. Reactivity was revealed with a human absorbed goat anti-rabbit IgG-PE or IgG-Alexa (Southern Biotechnology, Birmingham, Ala.). Preimmune serum of the same rabbit at the same dilution was used as a control. Samples were obtained using a FC-500 flow cytometer (BD) and analyzed using FCS express software.

Example 2

The inventors analyzed 163 human B-cell lymphomas for JAM-C expression (FIG. 3). The expression of JAM-C in B-cell lymphomas revealed a disease-specific pattern distinguishing JAM-Cpos from JAM-Cneg B-cell lymphomas. Therefore, JAM-C expression clearly allows the distinction between JAM-Cpos lymphomas (MZBL, MCL, HCL) and JAM-Cneg lymphomas (CLL, FL).

To determine JAM-C expression, cell samples were incubated with JAM-C rabbit antiserum at a dilution of 1:2000 after incubation with normal human serum. Reactivity was revealed with a human absorbed goat anti-rabbit IgG-PE or IgG-Alexa (Southern Biotechnology, Birmingham, Ala.). Preimmune serum of the same rabbit at the same dilution was used as a control. Samples were obtained using a FC-500 flow cytometer (BD) and analyzed using FCS express software.