Anti cd3 bv786

Manufactured by BioLegend

Sourced in United States

Anti-CD3 BV786 is a fluorochrome-conjugated monoclonal antibody that binds to the CD3 antigen on human T cells. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-CD3 (468 citations) CD3-BV786 (4 citations)

3 protocols using anti cd3 bv786

Intracellular Cytokine Profiling of T-Cell Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

The splenocyte-derived cytokines were measured using intracellular cytokine staining (ICS), as described in the Supplementary Materials. Briefly, splenocytes were collected 3 weeks after the third immunization and subsequently pulsed with an anti-CD28 agonist antibody (BD Biosciences, USA) in combination with either RBD or a set of 14-mer peptides covering the RBD region in overlap (NR-52402, BEI Resources, USA). After 1 h, the cells were treated with brefeldin A (BD Biosciences, USA) and incubated for 12 h at 37 °C and 5 % CO₂. Non-pulsed cells served as the negative control. Extracellular staining was performed using Live/Dead Fixable Aqua Dead, anti-CD3 BV786 (BioLegend, USA), anti-CD8 APC (BioLegend, USA), and anti-CD4 BV605 (BioLegend, USA). After washing, the fixed and permeabilized cells were intracellularly stained with anti-IFNγ PE (BioLegend, USA), anti-IL-2 BV421 (BioScience), and anti-TNFα Pe-Cy7 (BD Bioscience, USA). The cells were subsequently analyzed using an LSR Fortessa flow cytometer (BD Biosciences, USA).

Rodrigues-Jesus M.J., Teixeira de Pinho Favaro M., Venceslau-Carvalho A.A., de Castro-Amarante M.F., da Silva Almeida B., de Oliveira Silva M., Andreata-Santos R., Gomes Barbosa C., Brito S.C., Freitas-Junior L.H., Boscardin S.B, & de Souza Ferreira L.C. (2022). Nano-multilamellar lipid vesicles promote the induction of SARS-CoV-2 immune responses by a protein-based vaccine formulation. Nanomedicine, 45, 102595.

+ Open protocol

+ Expand

CFSE-Based T Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

HST cells were thawed and labeled with 0.5 µM CFSE in PBS. Cells were plated at 100,000 cells/well in 96-well round-bottomed plates in R10. Triplicate wells were prepared for each condition and stimulated with the indicated concentrations of TW10 peptide, with a concentration of DMSO matched to the 1 µg/ml peptide concentration, or with no additive in R10 medium. After 48 h of incubation at 37°C and 5% CO₂, media in all wells were replaced with R10 supplemented with 20 U/ml IL-2 (National Institutes of Health, National Cancer Institute Biorepositories and Biospecimen Research Branch) and cultured for an additional 3 d. Cells were then stained with anti-CD4 BV421, anti-CD8 BV605, anti-CD3 BV786 (all antibodies from BioLegend), and Live/Dead Aqua Fixable cell stain (Invitrogen) before being run on an Attune NxT flow cytometer (Thermo Fisher Scientific) and analyzed using FlowJo software (version 10). Degrees of proliferation were assessed by gating on lymphocytes (FSC/SSC), viable cells (Aqua stain negative), CD8⁺CD4⁻, and measuring the percentage of CFSE^dim (gate drawn based on DMSO control).

McCann C.D., van Dorp C.H., Danesh A., Ward A.R., Dilling T.R., Mota T.M., Zale E., Stevenson E.M., Patel S., Brumme C.J., Dong W., Jones D.S., Andresen T.L., Walker B.D., Brumme Z.L., Bollard C.M., Perelson A.S., Irvine D.J, & Jones R.B. (2021). A participant-derived xenograft model of HIV enables long-term evaluation of autologous immunotherapies. The Journal of Experimental Medicine, 218(7), e20201908.

+ Open protocol

+ Expand

Immune Cell Profiling in Immunized Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quadriceps muscles from immunized mice were harvested after 18–24 h of the injection and cut into small pieces. A single cell suspension was prepared using skeletal muscle dissociation kit (Miltenyi Biotec) according to manufacturer’s protocol. Briefly, small pieces of muscle were incubated in enzyme solution (enzyme D, enzyme P and enzyme A in DMEM) for 30 min at 37^oC with gentle shaking, passed through 70 µm cell strainer and wash with 10 ml DMEM. Centrifuge cell suspension at 300xg for 20 min. Cells were stained with a combination of the following fluorescently labeled antibodies: anti-CD45-AF700, anti-CD3-BV786, anti-CD19-BV605, anti-CD49b-PE, anti-CD11b-PE-Cy5, anti-CD11c-BV711, anti-F4/80-FITC, anti-Ly6C-PerCp, anti-Ly6G-PE/Cy7, anti-MHCII-PE/Dazzle594, anti-CD80-BV650, anti-CD40-APC, anti-CXCR3-BV510 and anti-CCR2-BV421 (all from BioLegend). Live/dead cells were analyzed using fixable viability dye efluor780 (eBioscience). The stained cells were acquired using BD LSRFortessa cell analyzer and data was analyzed using FlowJo software (Tree Star). Total immune cells (CD45⁺), myeloid cells (CD11b⁺), monocytes (CD11b⁺Ly6C⁺), macrophages (CD11b⁺F4/80⁺), neutrophils (CD11b⁺Ly6G⁺), dendritic cells (DCs; CD11c⁺MHCII⁺), natural killer cells (NK cells; CD49b⁺, CD3⁻), B cells (CD11b⁻CD11c⁻CD19⁺), T cells (CD11b⁻CD11c⁻CD3⁺) and NKT cells (CD49b⁺CD3⁺) were gated by flow cytometry.

Jain S., George P.J., Deng W., Koussa J., Parkhouse K., Hensley S.E., Jiang J., Lu J., Liu Z., Wei J., Zhan B., Bottazzi M.E., Shen H, & Lustigman S. (2018). The parasite-derived rOv-ASP-1 is an effective antigen-sparing CD4+ T cell-dependent adjuvant for the trivalent inactivated influenza vaccine, and functions in the absence of MyD88 pathway. Vaccine, 36(25), 3650-3665.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!