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C57bl 6 and c3h hej mice

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C57BL/6 and C3H/HeJ mice are commonly used laboratory mouse strains. C57BL/6 mice are a widely utilized inbred strain, while C3H/HeJ mice are inbred and known to have a genetic mutation that affects their immune response. These mice are often used in biomedical research, but a detailed description of their specific functions and intended uses is not provided to maintain an unbiased and factual approach.

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Lab products found in correlation

Cellcheck 19 plus, by IDEXX (2 mentions)

Close spelling variants of this product

C3H/HeJ mice C3H/HeJ (C3H) mice C3H/HeJ (C3H) C3H/HeJ C57BL/6 mice C3H mice C57BL/6

2 protocols using c57bl 6 and c3h hej mice

1

Syngeneic Fibrosarcoma Tumor Model

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Eight- to 10-week-old female C57BL/6 and C3H/HeJ mice (The Jackson Laboratory) were maintained in Association for Assessment and Accreditation of Laboratory Animal Care–accredited facilities and all procedures approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Pennsylvania. To propagate fibrosarcoma syngeneic tumors, 5 × 105 cells from a sarcoma line established from the LSL-KrasG12D/wt;p53FL/FL genetically engineered mouse model (GEMM) model (C57BL/6 background) at the fifth passage or 3 × 105 cells of the established RIF mouse sarcoma cell line (C3H/HeJ background, RRID:IMSR_JAX:000659) at the nineth passage were injected subcutaneously or intramuscularly in the right thigh. Cells from the LSL-KrasG12D/wt;p53FL/FL GEMM model were recently isolated for the purpose of this research. The established RIF cell line has been maintained in the laboratory of Dr. Busch for more than 20 years and originated from cells of the same line at Roswell Park Cancer Institute in Buffalo, NY. Both cell lines were authenticated and tested Mycoplasma negative by IDEXX BioAnalytics (CellCheck 19 plus) in 2021. Tumors were irradiated upon reaching 100 mm3 in volume. Tumor size was measured utilizing calipers and volume was calculated as (length × width2)/2.
Velalopoulou A., Karagounis I.V., Cramer G.M., Kim M.M., Skoufos G., Goia D., Hagan S., Verginadis I.I., Shoniyozov K., Chiango J., Cerullo M., Varner K., Yao L., Qin L., Hatzigeorgiou A.G., Minn A.J., Putt M., Lanza M., Assenmacher C.A., Radaelli E., Huck J., Diffenderfer E., Dong L., Metz J., Koumenis C., Cengel K.A., Maity A, & Busch T.M. (2021). FLASH Proton Radiotherapy Spares Normal Epithelial and Mesenchymal Tissues While Preserving Sarcoma Response. Cancer Research, 81(18), 4808-4821.
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2

Syngeneic Tumor Propagation in Mice

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Eight to 10-week-old female C57BL/6 and C3H/HeJ mice (The Jackson
Laboratory) were maintained in AALAC-accredited facilities and all procedures
approved by the Institutional Animal Care and Use Committee (IACUC) at the
University of Pennsylvania (Philadelphia, PA). To propagate fibrosarcoma
syngeneic tumors, 5×105 cells from a sarcoma line established
from the
LSL-KrasG12D/wt;p53FL/FLGEMM model (C57BL/6 background) at the 5th passage or
3×105 cells of the established RIF mouse sarcoma cell line
(C3H/HeJ background, RRID:IMSR_JAX:000659) at the 9th passage were
injected subcutaneously or intramuscularly in the right thigh. Cells from the
LSL-KrasG12D/wt;p53FL/FLGEMM model were recently isolated for the purpose of this research. The
established RIF cell line has been maintained in the laboratory of Dr. Busch for
more than 20 years and originated from cells of the same line at Roswell Park
Cancer Institute in Buffalo, NY. Both cell lines were authenticated and tested
mycoplasma negative by IDEXX BioAnalytics (CellCheck 19 plus) in 2021. Tumors
were irradiated upon reaching 100 mm3 in volume. Tumor size was
measured utilizing calipers and volume was calculated as: (Length ×
Width2)/2.
Velalopoulou A., Karagounis I.V., Cramer G.M., Kim M.M., Skoufos G., Goia D., Hagan S., Verginadis I.I., Shoniyozov K., Chiango J., Cerullo M., Varner K., Yao L., Qin L., Hatzigeorgiou A.G., Minn A.J., Putt M., Lanza M., Assenmacher C.A., Radaelli E., Huck J., Diffenderfer E., Dong L., Metz J., Koumenis C., Cengel K.A., Maity A, & Busch T.M. (2021). FLASH Proton Radiotherapy Spares Normal Epithelial and Mesenchymal Tissues While Preserving Sarcoma Response. Cancer Research, 81(18), 4808-4821.
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