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4 0 monocryl suture

Manufactured by Johnson & Johnson
Sourced in United States

The 4–0 Monocryl® sutures are a monofilament absorbable surgical suture material made from a copolymer of glycolide and ε-caprolactone. The sutures provide strength and flexibility for wound closure procedures.

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5 protocols using 4 0 monocryl suture

1

Rat Behavior Monitoring via Accelerometry

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A 6-axis gyroscope/accelerometer (InvenSense MPU-6050, San Jose, California, USA), configured to an acceleration sensing range ± 4 g, was sutured to the flank of each rat at the thoracolumbar junction using 4-0 monocryl suture (Ethicon, Somerville, NJ, USA). Accelerometry was recorded at 30 Hz for groups 1, 2, and 6. Given the minimal acceleration observations from these treatment groups, no further accelerometry measurements were collected.
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2

Tracing Vagus Nerve Pathways in Rats

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Each rat was given atropine (0.1 mg/ml, s.c.) 15 minutes prior to surgery to prevent airway secretions during surgery, anesthetized (4% isoflurane), laid supine and cervical portions of the left vagus nerve was isolated from surrounding tissues. A small piece of parafilm was placed under the left vagus nerve to minimize diffusion to surrounding tissues and the tract tracers IB4 (1 to 2 μl of a 4% solution in deionized water, Sigma-Aldrich) and CTb (1 to 2 μl of 1-2% solution in deionized water, List Biological Laboratories) were pressure injected slowly into the nerve with a single-barrel glass micropipette, 20 to 40 μm tip size, attached to a picospritzer (General Valve Inc.). Following injections, the parafilm was removed, surgical wounds were closed with 4-0 monocryl suture (Ethicon Inc.), and animals were kept warm on a heated pad to recover until they were returned to their home cage.
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3

Rabbit Calvarial Bone Defect Model

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The skin of the rabbit was incised from the nasal bone to the mid-sagittal crest, and the periosteum was elevated to expose the parietal bone. Two critical-size 10-mm diameter calvaria bone defects were prepared with a trephine under copious irrigation with sterile saline. Maximal care was taken to avoid injury to the dura mater. Allocation of the 5 applied treatment modalities, (1) NC, (2) DBBM, (3) α-TCP + DBBM, (4) BBCP_3 and (5) BBCP_6 (n = 6, each) was randomized according to the systematic random protocol (www.randomization.com (accessed on 17 January 2019)). The 600 μL of blood were collected from the auricular artery per animal. The 300 μL of blood were used to mix with each granule and implanted into a defect, and the 300 μL filled up into the NC. After implantation of the materials, the 12.5 mm × 13.0 mm-sized resorbable collagen barrier membrane (BioGide®, Geistlich Pharma AG, Wolhusen, Switzerland) was used to cover the defect sites. The wound was closed in two layers with interrupted sutures using 4–0 Vicryl® and 4–0 Monocryl® sutures (Ethicon, Somerville, NJ, USA), respectively. Wound surfaces were further sealed with a spray film dressing (OPSITE® SPRAY, Smith & Nephew, London, UK).
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4

Cranial Bone Defect Repair Protocol

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Skin was incised on a 3 cm straight line from the nasal bone to the midsagittal crest. Periosteum was elevated to allow exposure of the parietal bone. Two bone defects (10 mm) on each side of the midline were created with a trephine bur under irrigation with sterile saline. Care was taken to avoid injury of the dura. A drilling procedure with a trephine bur was done very carefully with low speed (maximum 800 rpm) by trained cranio-maxillofacial surgeons. Drilling was stopped before reaching the dura and the bone pieces were removed by blunt instruments such as mucosa elevators. Under the conditions of the experiment, fresh blood needed to be mixed with the biomaterial under investigation. Blood was therefore sampled from the catheterized auricular artery approximately half way through the procedure (arterial blood gases were analysed at the same time). Following mixture, the biomaterials were implanted directly into the bone defects. After implantation the periosteum and skin were closed with interrupted sutures in layers using 4–0 Vicryl and 4-0 Monocryl sutures (Ethicon, Somerville, NJ, USA). The wound surface was further protected with spray film dressing (Opsite® spray, Smith & Nephew, London, UK).
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5

Bone Regeneration with Chitosan Membranes

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The skin of the head was incised from the nasal bone to the mid-sagittal crest, and the parietal bone was exposed after the periosteum elevation. Two critical-sized (10 mm diameter) bone defects were prepared in the parietal bones with a trephine drill, with maximal care not to injure the dura mater. Both CM biomaterials were pre-shaped with a scalpel into 10 mm diameter and 3 mm thickness cylinder (Figure 2A), and implanted in the defects without excessive pressure (Figure 2B). The applied treatment modalities, (1) empty control (n = 6), (2) Ncl_CM (n = 8), and (3) Cl_CM (n = 8), were randomly allocated. The wound was sutured in two layers using 4–0 Vicryl® (Ethicon, Somerville, NJ, USA) and 4–0 Monocryl® sutures (Ethicon). Furthermore, the surfaces of the wound were covered with a spray film dressing (OPSITE® SPRAY, Smith & Nephew, London, UK).
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