Anti cd81 pe

For analysis, the cells were harvested and incubated with fluorescently labeled anti-human antibodies for 30 min at 4 °C in the dark. Each sample was stained by 1 ul of each antibody at a concentration of 0.2 mg/mL in 100 ul of PBS containing 2% FBS. In flow cytometry, live aAPCs or CD3+ Jurkat cells were gated and recorded in at least 1 × 10⁴ cells to determine the GFP expression rate. The following antibodies were used to detect targeting molecules: anti-CD80-PE (BioLegend, San Diego, CA, USA), anti-CD83-PE (BioLegend, San Diego, CA, USA), anti-CD137L-PE (BioLegend, San Diego, CA, USA), anti-CD-32-FITC (BioLegend, San Diego, CA, USA), anti-CD9-PE (BioLegend, San Diego, CA, USA), anti-CD63-PE (BioLegend, San Diego, CA, USA), anti-CD81-PE (BioLegend, San Diego, CA, USA), anti-CD8α-PerCP-cy5.5 (BioLegend, San Diego, CA, USA), and anti-CD3-BV421 (BioLegend, San Diego, CA, USA). Fluorescence was measured using a BD FACS Canto (BD Biosciences) and analyzed using the FlowJo v10 software (BD Biosciences).